基于LC/Q-TOF MS的芽胞杆菌代谢组学分析方法

史怀; 刘波; 陈峥; 刘国红; 潘志针; 陈梅春; 朱育菁

doi:10.19303/j.issn.1008-0384.2012.10.017

基于LC/Q-TOF MS的芽胞杆菌代谢组学分析方法

doi: 10.19303/j.issn.1008-0384.2012.10.017

福建省农业科学院农业生物资源研究所,福建福州,350003

基金项目:

国家“973”计划前期项目(2011CB111607)

国家“948”计划项目(2011-G25)

福建省青年科技创新基金项目(2011J05053)

详细信息

作者简介:
史怀(1978-),硕士,副研究员,主要从事农业微生物研究(E-mail:darkone@126.com);刘波(1957-),博士,研究员,主要从事微生物生物技术与农业生物药物研究(E-mail:fzliubo@163.com)

中图分类号: S182
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出版历程
- 收稿日期: 2012-08-27
- 刊出日期: 2012-10-18

Metabonomics Analysis of Bacillus based on LC/Q-TOF MS

Agricultural Bioresources Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou, Fujian 350003,China

摘要

摘要: 建立了基于LC/Q-TOF MS的代谢组学方法,对不同种的芽胞杆菌进行分析,获得具有显著差异性的潜在代谢标记物,并采用分层聚类和PCA数据处理方法对各芽胞杆菌进行分类,建立PLS-DA预测模型。结果表明,不同的芽胞杆菌种间具有显著性差异,该方法可从代谢水平上对芽胞杆菌种属进行分类,为芽胞杆菌的分类提供了一种新的技术手段。
- 芽胞杆菌 /
- 代谢组学 /
- 分析方法
Abstract: LC/Q-TOF MS has been applied to the metabonomics analysis of Bacillus.Significant potential metabolic markers were developed by analyzing different species of Bacillus,hierarchical clustering and PCA analysis were used for classification of various Bacillus and PLS-DA prediction model was established.The results showed that there were statistical difference between Bacillus mycoides and Bacillus subtilis.Resultssuggested that metabonomics was a potential classable method to the identification of bacillus.
- Bacillus /
- Metabonomics /
- analysis

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