鸽源鸭甲肝病毒1型的分离与鉴定

施少华; 陈红梅; 陈珍; 傅光华; 万春和; 程龙飞; 苏敬良; 黄瑜

doi:10.19303/j.issn.1008-0384.2012.11.002

鸽源鸭甲肝病毒1型的分离与鉴定

doi: 10.19303/j.issn.1008-0384.2012.11.002

施少华^1,2,
陈红梅^1,2,
陈珍¹,
傅光华^1,2,
万春和^1,2,
程龙飞^1,2,
苏敬良³,
黄瑜^1,2

1. 福建省农业科学院畜牧兽医研究所,福建福州,350013;
2. 福建省畜禽疫病防治工程技术研究中心,福建福州,350013;
3. 中国农业大学动物医学院,北京,100094

基金项目:

福建省自然科学基金项目(2011J01113)

国家自然科学基金项目(30471283)

福建省种业创新与产业化工程建设项目(2011FJZY-9-06)

详细信息

作者简介:
施少华(1977-),男,在读博士,副研究员,主要从事兽医微生物学研究;黄瑜(1965-),男,研究员,主要从事动物传染病研究(E-mail:huangyu_815@163.com)

中图分类号: S852.65
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出版历程
- 收稿日期: 2012-07-22
- 刊出日期: 2012-11-18

Isolation and Identification of Pigeion-originated Duck Hepatitis A Virus 1

1. Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;
2. Fujian Animal Diseases Control Techology Development Center,Fuzhou,Fujian 350013,China;
3. College of Veterinary Medicine, China Agricultural University,Beijing 100094,China

摘要

摘要: 为对1株鸽源鸭甲肝病毒1型(DHAV-1)进行分离与鉴定,通过细菌分离排除法和病毒分离方法获得致鸭胚死亡病毒(暂命名为FJ-12-20株)。该病毒无血凝活性,可被DHAV-1引物所扩增,但不能被鸭坦布苏病毒、番鸭呼肠孤病毒、番鸭细小病毒和鹅细小病毒特异引物所扩增,病毒液攻击樱桃谷鸭和番鸭可引起死亡,并出现了典型的鸭肝炎病变,重新分离获得病毒株。将DHAV-1特异性扩增产物回收后克隆,序列分析表明FJ-12-20株与参考株DHAV-1的同源率为93.5%~99%,与DHAV-2和DHAV-3参考株的同源率均为78.4%。遗传进化分析表明FJ-12-20株与DHAV-1关系密切,它们在进化树中共处一分支。
- 鸽源 /
- 鸭甲肝炎病毒1型 /
- 分离鉴定
Abstract: Duck hepatitis A virus 1(DHAV-1),FJ-12-20,was isolated from a dead pigeon in Fujian.The strain,which lacked the hemagglutination activity,was identified by using the specific primers of DHAV-1,against those of the duck Tembusu virus,duck reovirus,duck parvirus and goose parvirus.Some Cherry Valley ducks and Muscovy ducks died after being challenged by the allantoic fluid containing FJ-12-20.Those ducks characterized with overt lesions of duck hepatitis.The virus was also successfully recovered from the infected ducks,and confirmed by reverse transcription-PCR.Sequence analysis revealed a high homology(93.5%-99%) existed between FJ-12-20 and DHAV-1 from GenBank.A significantly lower homology(78.4%),on the other hand,was found between FJ-12-20 and the standard strain of DHAV-2 or DHAV-3.Phylogenetic analysis showed that FJ-12-20 was close to DHAV-1,and all of these viruses could be subdivided into an isolated cluster.
- pigeon-origin /
- duck hepatitis A virus 1 /
- isolation and identification

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