双孢蘑菇As2796全长cDNA文库的构建及鉴定

陈美元

doi:10.19303/j.issn.1008-0384.2012.11.012

双孢蘑菇As2796全长cDNA文库的构建及鉴定

doi: 10.19303/j.issn.1008-0384.2012.11.012

陈美元

福建省农业科学院食用菌研究所,福建福州,350014

基金项目:

福建省财政专项——福建省农科院科技创新团队建设项目(2012)

详细信息

作者简介:
陈美元(1972-),男,博士,高级工程师,主要从事食用菌遗传育种研究(E-mail:401061326@qq.com)

中图分类号: S646
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- 文章访问数: 97
- HTML全文浏览量: 26
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- 被引次数: 0
出版历程
- 收稿日期: 2012-10-11
- 刊出日期: 2012-11-18

Construction and Identification of Full-length cDNA Library of Agaricus bisporus As2796

CHEN Mei-yuan

Institute of Edible Fungi,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350014,China

摘要

摘要: 双孢蘑菇As2796是我国的主栽品种。为方便获得相关的目的基因,构建了该菌株的全长cDNA文库,文库初始库容达3.3×106 cfu.mL-1,插入片段长度范围0.7~4.0kb,平均长度约1.5kb,重组率约82%,全长率约35%。文库扩增出了5个目的基因全长编码序列(CDS),表明该文库的有效性,今后可用于扩增所需的双孢蘑菇基因的全长cDNA或CDS。
- 食用菌 /
- 基因文库 /
- 编码序列
Abstract: A.bisporus As2796 is the main cultivar in China.For easily cloning the related target genes,the full-length cDNA library of As2796 was constructed.The library had a content of 3.3×106 cfu·mL-1,an insertion range of about 0.7-4.0 kb,an average cDNA length of 1.5 kb,a recombination efficience of about 82%,and full-length ratio of about 35%.The coding sequences(CDS) of 5 target genes were got from the library by PCR amplification,which proved the validity of the library for amplifying full-length cDNA or CDS of A.bisporus genes in need.
- edible fungus /
- gene library /
- coding sequence(CDS)

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参考文献(15)

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