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青枯雷尔氏菌TN5转座子无致病力突变株插入位点的鉴定与分析

车建美 马桂美 刘波 郑雪芳 唐建阳

车建美, 马桂美, 刘波, 郑雪芳, 唐建阳. 青枯雷尔氏菌TN5转座子无致病力突变株插入位点的鉴定与分析[J]. 福建农业学报, 2012, 27(11): 1231-1236. doi: 10.19303/j.issn.1008-0384.2012.11.017
引用本文: 车建美, 马桂美, 刘波, 郑雪芳, 唐建阳. 青枯雷尔氏菌TN5转座子无致病力突变株插入位点的鉴定与分析[J]. 福建农业学报, 2012, 27(11): 1231-1236. doi: 10.19303/j.issn.1008-0384.2012.11.017
CHE Jian-mei, MA Gui-mei, LIU Bo, ZHENG Xue-fang, TANG Jian-yang. Identification and Analysis of Tn5 Transposed Insert Sites in Avirulent Mutants of Ralstonia solanacearum[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1231-1236. doi: 10.19303/j.issn.1008-0384.2012.11.017
Citation: CHE Jian-mei, MA Gui-mei, LIU Bo, ZHENG Xue-fang, TANG Jian-yang. Identification and Analysis of Tn5 Transposed Insert Sites in Avirulent Mutants of Ralstonia solanacearum[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1231-1236. doi: 10.19303/j.issn.1008-0384.2012.11.017

青枯雷尔氏菌TN5转座子无致病力突变株插入位点的鉴定与分析

doi: 10.19303/j.issn.1008-0384.2012.11.017
基金项目: 

国家自然科学基金项目(30871667)

“十二五”农村领域国家科技计划课题(2012AA101504)

国家“948”计划项目(2011-G25)

福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y03)

国家星火科技计划项目(S20110410006)

国家“973”计划前期项目(2011CB111607)

详细信息
    作者简介:

    车建美(1977-),女,副研究员,博士,研究方向:生物技术(E-mail:chum2002@163.com);刘波(1957-),男,博士,研究员,研究方向:微生物生物技术与农业生物药物(E-mail:liubofaas@163.com)

  • 中图分类号: S432.4

Identification and Analysis of Tn5 Transposed Insert Sites in Avirulent Mutants of Ralstonia solanacearum

  • 摘要: 利用TN5插入强致病力青枯雷尔氏菌FJAT-91,获得突变株FJAT-t582和FJAT-t583。菌落形态观察及弱化指数测定结果表明,突变株FJAT-t582和FJAT-t583表现为典型的无致病力菌落形态,且弱化指数均在0.8以上。利用卡那霉素抗性基因特异性引物从突变菌株FJAT-t582和FJAT-t583中均扩增出片段大小为681bp的条带,而野生型菌株FJAT-91未扩增出任何条带,说明TN5转座子已插入其基因组中。对这2株无致病力突变株进行反向PCR测序和序列比对,分析鉴定出插入位点在编码putative diguanylate phosphodiesterase基因和Ⅲ型效应蛋白基因中,推测这2个基因的突变致使强致病力菌株表现出无致病力特征。
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出版历程
  • 收稿日期:  2012-08-09
  • 刊出日期:  2012-11-18

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