农杆菌介导转mgfp4基因水稻的获得

周淑芬

doi:10.19303/j.issn.1008-0384.2012.12.004

农杆菌介导转mgfp4基因水稻的获得

doi: 10.19303/j.issn.1008-0384.2012.12.004

周淑芬

1. 福建省农业科学院生物技术研究所,福建福州,350003;
2. 福建省农业遗传工程重点实验室,福建福州,350003

基金项目:

福建省财政专项——福建省农业科学院科技创新团队建设项目(CXTD2011-09)

福建省自然科学基金项目(2009J01091)

详细信息

作者简介:
周淑芬(1978-),女,硕士,助理研究员,主要从事水稻分子生物学研究

中图分类号: S511
计量
- 文章访问数: 148
- HTML全文浏览量: 37
- PDF下载量: 0
- 被引次数: 0
出版历程
- 收稿日期: 2012-10-05
- 刊出日期: 2012-12-18

Transgenic Rice with mgfp4 Gene Mediated by Agrobacterium tumefaciens

ZHOU Shu-fen

1. Key Laboratory of Agricultural Genetic Engineering of Fujian Province,Institute of Biotechnology,Fuzhou, Fujian 350003,China;
2. Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

摘要

摘要: 通过农杆菌介导法将携带mgfp4基因的植物表达载体pCAGFPHyg导入水稻明恢86中,获得101个独立克隆的转基因植株。PCR检测和Southern blot分析证明外源基因已整合进水稻基因组中,T0代种子的分离分析结果表明大部分转基因植株为单T-DNA插入。荧光检测及RT-PCR分析结果显示mgfp4基因能在水稻中表达,但其发出的绿色荧光检测效果不甚理想。
- 水稻 /
- 绿色荧光蛋白基因 /
- 农杆菌介导 /
- 遗传转化
Abstract: Plant expression vector,pCAGFPHyg,carrying mgfp4 gene was introduced into rice Minghui 86(Indica Sativa L.) mediated by Agrobacterium tumefaciens.The transgenic rice containing 101 independent clones was obtained.PCR and the southern blot analysis ensured that the exogenous genes were indeed integrated into the rice genome.The result of T1 segregation analysis from T0 rice seeds indicated that most of the transgenic plants were inserted by a single T-DNA.It was confirmed by the fluorescence microscopic and RT-PCR detections that the mgfp4 gene was expressed in the transgenic rice,although no acceptable result of green fluorescence was secured.
- Rice /
- green fluorescent protein gene /
- Agrobacterium-mediated /
- genetic transformation

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参考文献(20)

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