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基因芯片技术在水稻抗逆基因表达研究中的应用

陈玉 刘鹏 陈由强 张建福 谢华安

陈玉, 刘鹏, 陈由强, 张建福, 谢华安. 基因芯片技术在水稻抗逆基因表达研究中的应用[J]. 福建农业学报, 2012, 27(12): 1395-1400. doi: 10.19303/j.issn.1008-0384.2012.12.023
引用本文: 陈玉, 刘鹏, 陈由强, 张建福, 谢华安. 基因芯片技术在水稻抗逆基因表达研究中的应用[J]. 福建农业学报, 2012, 27(12): 1395-1400. doi: 10.19303/j.issn.1008-0384.2012.12.023
CHEN Yu, LIU Peng, CHEN You-qiang, ZHANG Jian-fu, XIE Hua-an. Gene Chips for Expression of Stress-tolerant Genes in Rice[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1395-1400. doi: 10.19303/j.issn.1008-0384.2012.12.023
Citation: CHEN Yu, LIU Peng, CHEN You-qiang, ZHANG Jian-fu, XIE Hua-an. Gene Chips for Expression of Stress-tolerant Genes in Rice[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1395-1400. doi: 10.19303/j.issn.1008-0384.2012.12.023

基因芯片技术在水稻抗逆基因表达研究中的应用

doi: 10.19303/j.issn.1008-0384.2012.12.023
基金项目: 

福建省财政专项-福建省农业科学院科技创新团队项目(STIF-Y04)

国家“973”计划项目(2012CB723003)

福建省杰出青年基金项目(2009J06011)

国家自然科学基金项目(30871509)

国家“863”计划项目(2011AA10A101)

详细信息
    作者简介:

    陈玉(1986-),女,硕士研究生,主要从事水稻分子生物学研究;张建福(1971-),男,博士,副研究员,主要从事水稻分子生物学与分子育种研究(E-mail:jianfzhang@163.com);谢华安(1941-),男,研究员,主要从事水稻遗传育种研究(E-mail:huaanxie@yahoo.com.cn)

  • 中图分类号: S511

Gene Chips for Expression of Stress-tolerant Genes in Rice

  • 摘要: 水稻是世界上最重要的粮食作物之一,全世界50%以上的人口以水稻为主食,导致水稻减产的主要原因之一是受逆境胁迫的影响。为了提高水稻的抗逆性,目前国内外许多实验室正致力于水稻抗逆机制的研究。与抗逆相关的单一基因较难以揭示植物复杂的抗逆机制,而基因芯片技术却可以揭示大量基因表达和调控的情况,使得多基因的调控研究成为可能。本文主要综述了近年来基因芯片技术在水稻受逆境胁迫条件下基因的表达情况。
  • [1] GERSHON D.Microarray technology:an array of oppor-tunities[J].Nature,2002,416:885-891.
    [2] FORDOR S P,READ J L,PIRRUNG M C,et al.LighT-directed,spatially addressable paralled chemical synthesis[J].Science,1991,251:767-773.
    [3] SCHENA M,SHALON D,DAVIS R W,et al.Quantitativemonitoring of gene expression patterns with a complementaryDNA microarray[J].Science,1995,270(5235):368-371.
    [4] 林海建,张志明,沈亚欧,等.基因芯片研究植物逆境基因表达新进展[J].遗传,2009,31(12):1192-1204.
    [5] 张津津,严成其,金志华,等.基因芯片技术在水稻研究中应用进展[J].浙江农业学报,2008,20(4):308-311.
    [6] MENGEL K,KIRKBY E A.Principles of Plant Nutrition[J].Bern,Switzerland:International Potash Institute,1979.
    [7] FERNANDES M S,PEREYRA ROSSIELLO R O.Mineralnitrogen in plant physiology and plant nutrition[J].CriticalRev Plant Sci,1995,14(2):111-148.
    [8] MORI S.Iron acquisition by plants[J].Curr Opin Plant Biol,1999,2(3):250-253.
    [9] WASAKI J,YONETANI R,SHINANO T,et al.Expressionof the OsPI1 gene,cloned from rice roots using cDNAmicroarray,rapidly responds to phosphorus status[J].NewPhytol,2003,158:239-248.
    [10] WASAKI J,YONETANI R,KURODA S,et al.Transcriptomic analysis of metabolic changes by phosphorusstress in rice plant roots[J].Plant Cell Environ,2003,26(9):1515-1523.
    [11] 李利华,邱旭华,李香花,等.水稻低磷胁迫基因表达谱分析[J].科学通报,2010,55(3):212-219.
    [12] CAI H M,XIE W B,ZHU T,et al.Transcriptome responseto phosphorus starvation in rice[J].Acta Physiol Plant,2012,34(1):327-341.
    [13] DAI X Y,WANG Y Y,YANG A,et al.OsMYB2P-1,an R2R3MYB Transcription Factor,Is Involved in the Regulation ofPhosphate-Starvation Responses and Root Architecture in Rice[J].Plant Physiology,2012,159:169-183.
    [14] LIAN X M,WANG S P,ZHANG J W,et al.Expressionprofiles of 10,422genes at early stage of low nitrogen stressin rice assayed using a cDNA microarray[J].Plant Mol Biol,2006,60(5):617-631.
    [15] 赵明辉,孙建,王嘉宇,等.全基因组分析低氮胁迫下水稻剑叶光合相关基因表达变化[J].中国农业科学,2011,44(1):1-8.
    [16] 裴雁羲,董海涛,李德葆.白叶枯病菌诱导水稻特异基因表达的微阵列分析[J].农业生物技术学报,2002,10(4):321-326.
    [17] 刘峙,刘莉,付月灵,等.Pant MetGenMAP数据库分析水稻表达谱芯片数据的方法初探[J].植物保护,2011,37(3):104-108.
    [18] 饶志明,董海涛,庄杰云,等.水稻抗稻瘟病近等基因系的cDNA微阵列分析[J].遗传学报,2002,29(10):887-893.
    [19] 庄晓峰,董海涛,李德葆.水稻抗病性反应的cDNA微阵列分析及一个新基因OsBTB的发现[J].植物病理学报,2005,35(3):221-228.
    [20] SONIA G M,CHANG H S,ZHU T,et al.ComparativeTranscriptomics of rice reveals an ancient pattern of responseto microbial colonization[J].PNAS,2005,102:8066-8070.
    [21] TANAKA N,CHE F S,WATANABE N,et al.Flagellinfrom an incompatible strain of Acidovorax avenae mediates H2O2generation accompanying hypersensitive cell death andexpression of PAI,ChtIand PBZIbut not of Loxin rice[J].Molecular PlanT-Microbe Interact,2003,16:422-428.
    [22] FUJIWARA S,TANAKA N,KANEDA T,et al.RicecDNA microarry-BAsed gene expression profiling of theresponse to flagellin perception in cultured rice cells[J].Molecular PlanT-Microbe Interaction,2004,17:986-98.
    [23] LI Q,CHEN F,SUN L,et al.Expression profiling of ricegenes in early defense responses to blast and bacterial blightpathogens using cDNA microarray[J].Physiological andMolecular Plant Pathology,2006,68:51-60.
    [24] SANTOSH K G,AMIT K R,SHAMSHER S K,et al.Thesingle functional blast resistance gene Pi54activates a complexdefence mechanism in rice[J].Journal of ExperimentalBotany,2012:63(2)757-772.
    [25] SHINJI K,CHRIS B,MICHAEL D,et al.GeneExpression Profiles during the Initial Phase of Salt Stress inRice[J].The Plant Cell,2001,4(13):889-905.
    [26] WALIA H,WILSON C,CONDAMINE P,et al.Comparative transcriptional profiling of two contrasting ricegenotypes under salinity stress during the vegetative growthstage[J].Plant Physiol,2005,139(2):822-835.
    [27] UEDA A,KATHIRESAN A,BENNETT J,et al.Comparative transcriptome analyses of barley and rice undersalt stress[J].Theor Appl Genet,2006,112(7):1286-1294.
    [28] ASHIQ R,KYONOSHIN M,HIROSHI A,et al.Monitoring expression profiles of rice genes under cold,drought,and high-salinity stresses and abscisic acid applicationusing cDNA microarry and RNA gel-blot analyses[J].PlantPhysiology,2003,133:1755-1767.
    [29] 马廷臣,陈荣军,余蓉蓉,等.渗透胁迫下水稻根系核仁小分子RNA转录本变化的全基因组表达分析[J].中国水稻科学,2012,26(1):l6-20.
    [30] ZHAO B,LIANG R,Ge L,et al.Identification of droughtinduced microRNAs in rice[J].Biochemical and BiophysicalResearch Communications,2007,354:585-590.
    [31] YAMAGUCHI T,NAKAYAMA K,HAYASHI T,et al.cDNA microarray analysis of rice anther genes under chillingstress at the microsporogenesis stage revealed two genes withDNA transponson castaway in 5′-flanking region[J].BiosciBiotechnol Biochem,2004,68(6):1315-1323.
    [32] 徐孟亮,李落叶,陈荣军,等.一个新的水稻低温应答类糖基转移酶基因(OsCrGtl)的表达分析与克隆[J].农业生物技术学报,2010,18(4):663-669.
    [33] YU J,HU S,WANG J,et al.A draft sequence of the ricegenome(Oryza sativa L.ssp Indica)[J].Science,2002,296:79-92.
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出版历程
  • 收稿日期:  2012-10-18
  • 刊出日期:  2012-12-18

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