BSA-ISCOMs对大黄鱼免疫机能及抗病力的影响

陈秀霞; 黄殿盛; 柯翎; 许斌福; 方冬兰; 龚晖

doi:10.19303/j.issn.1008-0384.2013.03.002

BSA-ISCOMs对大黄鱼免疫机能及抗病力的影响

doi: 10.19303/j.issn.1008-0384.2013.03.002

福建省农业科学院生物技术研究所,福建福州,350003

基金项目:

福建省海洋与渔业厅重点项目([2010]2-13)

福建省科技平台建设项目(2009N2003)

福建省科技计划项目——省属公益类科研院所基本科研专项(2011R1021-7、2009R10035-5)

详细信息

作者简介:
陈秀霞(1977-),女,副研究员,主要从事鱼类免疫制剂研究(E-mail:xiuxiachen@163.com);黄殿盛(1986-),男,硕士,主要从事鱼类免疫学研究。黄殿盛与陈秀霞对本文内有同等贡献;龚晖(1971-),男,研究员,主要从事鱼类病原学与免疫学研究(E-mail:ghxfjm@163.com)

中图分类号: S948
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出版历程
- 收稿日期: 2012-12-27
- 刊出日期: 2013-03-18

Effects of BSA-ISCOMs on Immunity Response and Disease Resistance of Pseudosciaena crocea

Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

摘要

摘要: 利用免疫刺激复合物(ISCOMs)非特异免疫增强作用和可经黏膜呈递的特点,将BSA制备成ISCOMs(BSA-ISCOMs),大黄鱼连续口服免疫10d。免疫后第15d和30d采血,测血清部分非特异性免疫指标。结果显示,免疫后第15d,试验组血清SOD、补体C3、总蛋白含量显著高于对照组(P<0.05),γ-干扰素高于对照组,但差异不显著(P>0.05),溶菌酶则显著低于对照组(P<0.05);免疫后第30d,除溶菌酶外,试验组各指标与对照组差异不显著(P>0.05)。免疫完成后第30d,创伤弧菌(Vibrio vulnificus)攻毒结果显示,对照组死亡率100%,试验组死亡率26.7%,相对免疫保护率为73.3%。因此,口服BSA-ISCOMs在一定药效期限内能有效提高大黄鱼的免疫机能,增强抗病能力。
- 牛血清白蛋白(BSA) /
- 免疫刺激复合物(ISCOMs) /
- 大黄鱼 /
- 非特异性免疫
Abstract: Immune stimulating complex(ISCOMs) is an efficient mucosal delivery system which can induce non-specific immune response.Bovine serum albumin(BSA) was incorporated into ISCOMs(BSA-ISCOMs).Pseudosciaena crocea took diets containing BSA-ISCOMs for 10 d,while the control group took diets containing PBS.At the 15th and 30th day after oral completion,serum was collected for non-specific immune parameters analysis.The results showed that at 15th day after oral completion,SOD,C3 and total protein of the fish feeding BSA-ISCOMs were significantly higher than those of the control fish(P<0.05),and interferon γ of the former was higher than that of the latter,but not significantly(P>0.05),while lysozyme of the former was significantly lower than that of the latter(P<0.05).At 30th day after oral completion,these parameters except lysozyme of the experimental group were not significantly different from the control group(P>0.05).The results of the challenge with Vibrio vulnificus at 30th day after oral completion showed that the cumulative mortality of the control and the experimental group was 100% and 26.7%,respectively,and the relative percent survival(RPS) was 73.3%.So this study indicated that BSA-ISCOMs can induce the immune response and improve the disease resistance of Pseudosciaena crocea.
- bovine serum albumin(BSA) /
- immune stimulating complex(ISCOMs) /
- Pseudosciaena crocea /
- non-specific immunity

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