青枯雷尔氏菌致病性的代谢组学异质性研究

史怀; 刘波; 陈峥; 郑雪芳; 潘志针; 陈梅春; 朱育菁

doi:10.19303/j.issn.1008-0384.2013.04.009

青枯雷尔氏菌致病性的代谢组学异质性研究

doi: 10.19303/j.issn.1008-0384.2013.04.009

福建省农业科学院农业生物资源研究所,福建福州,350003

基金项目:

国家自然科学基金项目(30871667)

福建省自然科学基金项目(2009J01087)

国家“863”计划项目(2012AA101504)

福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y03)

详细信息

作者简介:
史怀(1978-),硕士,副研究员,主要从事农业微生物研究(E-mail:darkone@126.com);刘波(1957-),博士,研究员,从事微生物生物技术与农业生物药物研究(E-mail:fzliubo@163.com)

中图分类号: S476.1
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出版历程
- 收稿日期: 2012-11-20
- 刊出日期: 2013-04-18

Metabonomic Heterogeneity Analysis for Pathogenicity of Ralstonia solanacearum

Agricultural Bioresources Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

摘要

摘要: 采用基于LC/Q-TOF MS的代谢组学方法,对不同致病性青枯雷尔氏菌的胞外代谢物进行了分析,获得了具有显著性差异的代谢标志物,并采用PCA数据处理方法对不同致病性菌株进行分类,建立致病性的PLS-DA预测模型。结果表明,该方法可以从代谢水平上区分野生型强致病力菌株、野生型无致病力菌株以及强致病力菌株经Tn-5转座子插入导致致病力丧失的人工致弱突变菌株。对代谢标志物的差异分析结果表明,在无致病力菌株和Tn-5致弱菌株中,有12种代谢标志物与强致病力菌株具有显著性差异(P<0.05,FC≥2),并表现出相同的丰度变化趋势,预示着这些代谢标志物可能与青枯雷尔氏菌的致病性有关。
- 代谢组学 /
- 青枯雷尔氏菌 /
- 致病性
Abstract: The metabolites of Ralstonia solanacearum with various pathogenic were analysed by metabonomic method based on LC/Q-TOF MS.Significant metabolic markers were used for classification pathogenic of Ralstonia solanacearum by PCA analysis,and PLS-DA prediction model were established.The result suggest that metabonomic analysis could be used for indentify virulent Ralstonia solanacearum strain and avirulent strain and Tn-5mutant strains.Furthermore,there were 12metabolites showed similar trend and had significant differences between virulent strain and avirulent strains(both wide-type and Tn-5mutant),suggesting that the 12metabolites are probably related to the pathogincity of Ralstonia solanacearum.
- metabonomic /
- ralstonia solanacearum /
- pathogenicity

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参考文献(26)

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