湿地松体胚发育成熟的影响因子研究

吴丽君; 翁秋媛; 陈达

doi:10.19303/j.issn.1008-0384.2013.04.014

湿地松体胚发育成熟的影响因子研究

doi: 10.19303/j.issn.1008-0384.2013.04.014

福建省林业科学研究院,福建福州,350012

基金项目:

国家林业局948项目(2009-4)

详细信息

作者简介:
吴丽君(1968-),女,教授级高级工程师,博士,主要从事林木、花卉生物技术研究

中图分类号: S791.246
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- 被引次数: 0
出版历程
- 收稿日期: 2013-03-14
- 刊出日期: 2013-04-18

Factors Affecting Maturation of Somatic Embryos of Slash Pine

Fujian Academy of Forestry,Fuzhou,Fujian 350012,China

摘要

摘要: 以湿地松4个胚性细胞系(即稳定增殖的胚性胚柄细胞团)为材料,开展体胚发育成熟的关键影响因子研究。结果表明:不同的胚性细胞系和成熟培养基中碳源种类是影响湿地松体胚成熟的关键因素;成熟培养基中PEG分子量大小及PEG、ABA、AC不同浓度组合影响成熟体胚的数量和质量;体胚成熟较佳培养基为LP+ABA70mg.L-1+PEG 8000150g.L-1+麦芽糖60g.L-1+AC 0~2.0g.L-1+琼脂粉3.0g.L-1,直径1cm大小胚性胚柄细胞团可获得成熟子叶胚19.8个。
- 湿地松 /
- 体胚成熟 /
- 影响因子
Abstract: Using 4embryogenesis cell lines(ECLs),which were the embryogenic suspensor mass(ESM)of the slash pine,critical factors affecting the maturation of somatic embryos(SEs)were identified and studied.The results showed that different ECLs and carbon sources in the medium were the key factors affecting the maturation of the SEs.The presence of PEG of different molecular weights in different concentrations with ABA and AC in the medium affected SEs both qualitatively and quantitatively.The most desirable medium for the embryo maturation was found to consist of LP+ABA70mg.L-1+PEG 8000 150g.L-1+maltose 60g.L-1+AC 0~2.0g.L-1+ agar 3.0g.L-1.On which,approximately 19.8mature cotyledonary embryos were harvested from 1cm diameter of ESM.
- Pinus elliottii /
- maturation of somatic embryos /
- factors

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参考文献(17)

[1]	LIAO Y K,AMERSON H V.Slash pine somatic embryogenesis:initiation of embryogenesis from immature zygotic embryos[J].New Forests,1995,(10):145-163.
[2]	LIAO Y K,AMERSON H V.Slash pine somaticembryogenesis:maturation of somatic embryos and plantregeneration[J].New Forests,1995,(10):165-182.
[3]	GUPTA P K,NEWTON R J.Somatic embryoryogenesis inwoody plants[M].London:Kluwer Academic Publishers,1995,90-108.
[4]	唐巍,欧阳藩.湿地松体细胞胚胎发生和植株再生[J].植物资源与环境,1997,6(2):8-11.
[5]	PULLMAN G S,NAMJOSHI K,ZHANG Y.Somaticembryogenesis in loblolly pine:improving culture initiationwith abscisic acid and silver nitrate[J].Plant Cell Reports,2003,(22):85-95.
[6]	PULLMAN G S,JOHNSON S,VAN TASSEL S,et al.Somatic embryogenesis in loblolly pine and Douglas fir:improving culture initiation and growth with MES pH buffer,biotin and folic acid[J].Plant Cell,2005,(80):91-103.
[7]	廖宇赓.台湾云杉(Picea morrisonicola Hay.)体胚发生与植株再生[J].中华林学,1999,32(2):161-170.
[8]	ATTREE S M,MOORE D,SAWHNEY V K.Enhancematuration and desiccation tolerance of white spruce somaticembryos:Effect of a non-plasmolyzing water stress and abscisicacid[J].Ann Bot,1991,(68):519-525.
[9]	吴丽君.湿地松合子胚发育与体胚诱导的相关性研究[J].福建林学院学报,2009,29(3):243-246.
[10]	吴丽君.湿地松体胚增殖与成熟培养研究[J].福建农业学报,2010,25(2):217-221.
[11]	IRAQI D,TREMBLAY T M.The role of sucrose duringmaturation of black spruce and white spruce somatic embryos[J].Physiol Plant,2001,111:381-388.
[12]	KONG L S,ATTREE S M,FOWKEL C.Changes ofendogenous hormone levels in developing seeds,zygoticembryos and megagametophytes in Picea glauca[J].Physiologia Plantarum,1997,101(1):23-30.
[13]	黄学林,李筱菊.高等植物组织离体培养的形态建成及其调控[M].北京:科学出版社,1995.
[14]	PULLMAN G S,JOHNSON S,PETER G.Improvingloblolly pine somatic maturation:comparison fo somatic andzygotic morphology,germination,and gene expression[J].Plant Cell Reports,2003,(21):747-758.
[15]	BECWAR M R,NAGMANI R,WANN S R.Initiation ofembryogenic cultures and somatic embryo development inloblolly pine[J].Can J For Res,1990,(20):810-817.
[16]	GUPTA P K,DURZAN D J.Biotechnology of somaticpolyembryogenesis and plantlet regeneration in loblolly pine[J].Bio Technology,1987,(5):147-151.
[17]	BECWAR M R,PULLMAN G S.Somatic embryogenesis inLoblolly pine[M].London:Kluwer Academic Publishers,1995.