Extraction and Deproteinization Technology for Crude Polysaccharides in Radix Pseudostellariae
-
摘要: 太子参多糖是太子参的有效成分之一。对太子参中粗多糖的热水浴加热提取工艺进行研究,对料液比、提取温度、提取时间、提取次数分别进行单因素试验和L9(34)正交试验,结果表明最佳工艺料液比1∶20,温度100℃,回流提取90min,提取3次。用Sevage法、三氯乙酸法、三氯乙酸-正丁醇法3种方法对太子参粗多糖去除蛋白处理效果进行比较,结果显示三氯乙酸-正丁醇法的蛋白质去除效果较好,其多糖纯度为81.2%,蛋白含量为9.93%,蛋白去除率为60.14%。Abstract: Polysaccharide is one of the active ingredients extracted fromradix pseudostellariae.In this study,the solid-liquid ratio,temperature,extracting duration and times in crude polysaccharide extraction of radix pseudostellariae were investigated by hot water bath,using both the single-factor test and L9(34)orthogonal test.The results showed that the optimum parameters for extraction were with the ratio of solid to liquid 1∶20,extracting temperature 100℃,reflux extraction time 90min and extraction for 3times.The deproteinization effects were compared among three methods,the Sevag reagent,trichloroacetic acid,and trichloroacetic acid-butyl alcohol,and the results presented that trichloroacetic acid-butyl alcohol was the best for deproteinization,with 81.2% crued polysacchsrides and 9.93% protein,indicating that the deproteinization rate was 60.14%.
-
Key words:
- radix pseudostellariae /
- crued polysacchsrides /
- deproteinization
-
[1] 晏春耕.药用植物太子参的研究及其应用[J].现代中药研究与实践,2008,22(2):61-65. [2] 钟方小,彭广芳,李贵海.山东太子参多糖含量对质量的影响[J].中草药,1997,28(7):428. [3] CHU M,QI X X,ZHU F,et al.Review of polysacchariges[J].Research and Information on Chinese TraditionalMedicinea,2003,5(4):18. [4] 程黎晖.太子参化学成分、药理作用及临床应用研究近况[J].浙江中医杂志,2008,43(5):307-309. [5] 白东青,吴旋.枸杞多糖/灵芝多糖对金丝鱼生化指标的影响[J].中国饲料,2011,(1):30-32. [6] 白东清,吴旋.枸杞多糖对黄颡鱼免疫细胞活性的影响[J].水产科技情报,2011,38(1):6-9. [7] 王远吉.枸杞多糖对鲫鱼血清溶菌酶活性的影响[J].中国饲料,2004,(24):28,31. [8] 庞素风,吴志新,王艳,等.云芝多糖对银鲫非特异性免疫功能的影响[J].淡水渔业,2008,38(2):17-22. [9] 康洁.马齿苋多糖对草鱼的免疫调节作用[J].贵州农业科学,2012,40(7):162-164. [10] 王庆奎,赵海运.口服当归多糖对点带石斑鱼非特异性免疫力的影响[J].安徽农业科学,2011,39(22):13857-13860. [11] 王庆奎,赵海运.茯苓多糖对点带石斑鱼非特异性免疫力的影响[J].饲料工业,2011,32(14):34-36. [12] 王文平,郭祀远,李琳,等.苯酚-硫酸法测定野木瓜中多糖含量的研究[J].食品科学,2007,28(4):276-279. [13] 罗芳.Folin-酚试剂法蛋白质定量测定[J].黔南民族师范学院学报,2005,(3):46-47. [14] 赵卫星.超声波提取太子参多糖工艺的优化[J].时珍国医国药,2012,23(8):1972-1973. [15] 潘兴桥,李建萍,荆旭慧,等.超高压技术提取太子参多糖的工艺研究[J].宁夏农林科技,2012,53(11):93-95. [16] 张陆军,丁怡,陈芸芸,等.太子参多糖制备工艺研究[J].中国中药杂志,2004,29(12):1201-1203. [17] 谢丽源,彭卫红,甘炳成.桑黄多糖脱蛋白方法与条件优化[J].西南农业学报,2011,24(1):363-365. [18] 方升平,王维香,雒小龙.川芎多糖除蛋白方法研究[J].时珍国医国药,2009,20(9):2176-2177. -
点击查看大图
计量
- 文章访问数: 140
- HTML全文浏览量: 35
- PDF下载量: 0
- 被引次数: 0
下载:
