金针菇菌株的SCAR标记分子鉴别

王翠; 谢宝贵; 陈梁军; 柯汀; 柯伙钊

doi:10.19303/j.issn.1008-0384.2013.10.007

金针菇菌株的SCAR标记分子鉴别

doi: 10.19303/j.issn.1008-0384.2013.10.007

1. 福建生物工程职业技术学院,福建福州,350002;
2. 福建农林大学菌物研究中心,福建福州,350002

基金项目:

福建省中青年教师教育科研项目A类 (JA13413)

详细信息

作者简介:
王翠 (1984-), 女, 硕士研究生, 研究方向:食用菌种质资源与遗传育种;柯伙钊 (1964-), 男, 副教授, 研究方向:化学检测

中图分类号: S646.15
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出版历程
- 收稿日期: 2013-08-03
- 刊出日期: 2013-10-18

SCAR Molecular Identification of 30 Flammulina velutipes

1. Fujian Vocational College of Bioengineering;
2. Mycological Research Center of Fujian Agriculture and Forestry University

摘要

摘要: 利用前期开发的16个SCAR分子标记对不同来源的30株金针菇Flammulina velutipes菌株进行扩增分析。基于SCAR标记在检测菌株中的存在与缺失差异, F0026、F0114菌株被首先鉴别出来, 其次F0030+1、F0102、F0118这3个菌株被鉴别出来, 其余25株菌株除F0050与F0148、F0014与F0149两对菌株外, 21株菌株被依次鉴别出来。结果表明, 利用SCAR分子标记可以高效、快速、准确地用于金针菇生产菌株的分类鉴别。
- 金针菇 /
- SCAR分子标记 /
- 分子鉴定
Abstract: Thirty Flammulina velutipes strains were collected and analyzed with 16previously developed sequence characterized amplified regions (SCAR) molecular markers.Based on differences detected in SCAR phenotypes, two F.velutipes strains F026and F0114were identified firstly, three strains F0030+1, F0102and F0118were identified secondly and the remaining 21strains were identified successively.The results showed that SCAR molecular markers conld guarantee efficient, rapid, accurate identification of Flammulina velutipes strains.
- Flammulina velutipes /
- SCAR molecular marker /
- molecular identification

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参考文献(13)

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