摘要:
选取均匀分布于水稻12条染色体上的134对SSR(Simple Sequence Repeats,SSR)标记,对明恢63、明恢86、航1号和航2号等4个具有相似遗传背景的水稻骨干恢复系进行遗传多态性分析。结果表明,经航天诱变选育的“航1号、航2号”与原种明恢86之间存在不同程度的遗传多样性。在筛选的134对SSR引物中,具有多态性标记为39对,多态性频率为29.10%。每个标记位点的平均等位基因数为2.10个,变幅为1~4个,平均多态性信息含量指数(PIC)为0.37。聚类分析结果表明,当以0.87为阀值时可将航天诱变选育的“航1号、航2号”与原种“明恢86”区分开。其中在标记RM234两侧邻近的7个含有重要基因区段的SSR标记中有5个标记存在多态性,表明明恢86在航天诱变过程中可能在具有重要生物学功能的基因座发生了突变,再经系统选育成航1号、航2号优良恢复系,为航天诱变中因发生有利变异而选育新品种提供分子证据。
Abstract:
The genetic evolution analysis of four mainly restore lines for hybrid rice including Minghui63,Minghui86,Hang1and Hang2with similarly genetic background were carried out in the paper.A total of 134pairs of simple sequence repeats(SSR)were selected distributed evenly from twelve chromosomes of rice(Oryzae sativa L.).The results indicated that there were a lot of polymorphic loci between breeding varieties under space flight mutation(Hang1,Hang2)and original variety(Minghui86),39polymorphic loci with a polymorphic ratio of29.10%.The number of alleles per loci ranged from 1to 4with an average of 2.1.The value of polymorphism information content(PIC)was 0.37.The four mainly restore lines were classified into 2groups by clustering.It is distinction between Hang 1,Hang2and Minghui 86when the threshold value is 0.87.A further analysis on SSR markers distributed in two sides of marker RM234 which have key gene regions,indicated that there were 5polymorphism sites from 7pairs of SSR markers.Bioinformatics analysis showed that those loci have important biological function in Minghui 86,was mutated under space flight mutation.Furthermore,breeding excellent restoring line Hang 1and Hang 2,provided a molecular evidence that advantageous variations are in space flight mutation for rice breeding.
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