A Preliminary Report on Aseptic Seeding and Rapid Propagation of Pholidota cantonensis Rolfe
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摘要: 为解决细叶石仙桃人工栽培种苗短缺问题, 以细叶石仙桃蒴果为材料, 应用植物组织培养技术, 筛选不同阶段所需的最适培养基。结果表明其种子可在MS+20g·L-1蔗糖+8g·L-1琼脂培养基上萌发, 发芽率可达70%以上;MS+50g·L-1土豆+20g·L-1蔗糖+8g·L-1琼脂培养基有利于原球茎分化小苗, 低浓度6-BA和NAA组合适合原球茎增殖与芽的分化;在1/2MS+0.5mg·L-1 NAA+20g·L-1蔗糖+8g·L-1琼脂培养基上生根率达100%, 通过以上系列培养基的培养可获得完整植株。Abstract: To alleviate a seed shortage on Pholidota cantonensis Rolfe, artificial culturing with medium optimization was investigated by using the plant tissue culture technology.The results showed that MS+20g·L-1 sucrose+8g·L-1 agar was an appropriate medium that allowed a germination rate above 70%.MS+50g·L-1 potato+20g·L-1 sucrose+8g·L-1 agar was desirable for the plantlet′s protocorm differentiation.Low concentrations of 6-BA and NAA in medium was better for bud protocorm proliferation and differentiation.The medium formulated with 1/2MS+0.5mg·L-1 NAA+20g·L-1 sucrose+8g·L-1 agar was optimal for rooting, as a rate of 100%was achieved.By using the combination of the above-mentioned media at respective stages, the plantlets of P.cantonensis Rolfe could be rapidly obtained from seeds.
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Key words:
- Pholidota cantonensis Rolfe /
- seed /
- aseptic seeding /
- rapid propagation
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[1] 陈心启, 吉占和, 郎楷永, 等.中国植物志[M].18卷.北京:科学出版社, 1999:392-393. [2] 林来官, 张永田.福建植物志[M].第六卷.福州:福建科学技术出版社, 1994:636-638. [3] 王莲辉, 姜运力.尖叶石仙桃的胚培养与快速繁殖[J].植物生理学通讯, 2006, 42 (5) :136-137. [4] 曾碧玉, 朱根发, 刘海涛, 等.4种野生兰花种子特征及离体培养初报[J].亚热带植物科学, 2008, 37 (3) :31-34. [5] 廖彭莹, 李兵, 苗伟生, 等.细叶石仙桃挥发油成分的GC-MS分析[J].安徽农业科学, 2011, 39 (22) :13394-13395. [6] 沈宗根, 吕洪飞, 程存归.3种石斛属植物和细叶石仙桃的红外光谱分析和鉴定[J].西北植物学报, 2008, 28 (1) :97-102. [7] 祁献芳, 李东, 康文艺.细叶石仙桃地上部分脂溶性成分GCMC分析[J].河南大学学报:医学版, 2011, 30 (2) :90-92. [8] 建晨, 冯丽, 野原稔弘.细叶石仙桃的化学成分[J].中国中药杂志, 2008, 33 (14) :1691-1693. [9] 丽聪, 吴春敏, 陈海滨, 等.RP-HPLC法测定石仙桃中天麻素和天麻苷元[J].中草药, 2008, 39 (2) :283-285. [10] 林丽聪, 张怡平, 吴春敏, 等.石仙桃叶化学成分研究[J].时珍国医国药, 2009, 20 (4) :922-923. [11] LIU J X, ZHOU L, ZHOU Q, et al.Animal Experiment of the Fatigue-resisting and Anoxia-resisting Actions of Pholidota Chinensis Lindl[J].Chinese Journal of Clinical Rehabilitation, 2006, 10 (7) :157-159. [12] 刘洪旭, 吴春敏, 林丽聪, 等.石仙桃镇痛有效提取部位研究[J].福建中医学院学报, 2004, 14 (4) :34-36. [13] 刘建新, 周青, 连其深.石仙桃的镇痛作用的研究[J].赣南医学院学报, 2002, 22 (2) :105-107. -
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