湿度对田间自然热罩防治柑橘黄龙病的影响

林雄杰; 范国成; 胡菡青; 王贤达; Yulu Xia; 蔡子坚; 刘波

doi:10.19303/j.issn.1008-0384.2014.05.014

湿度对田间自然热罩防治柑橘黄龙病的影响

doi: 10.19303/j.issn.1008-0384.2014.05.014

1. 福建省农业科学院果树研究所,福建福州,350013;
2. The United States Department of Agriculture，Animal and Plant Health Inspection Service，Plant Protection and Quarantine，Center for Plant Health Science and Technology，Raleigh，NC27606，USA;
3. 福建省农业科学院,福建福州,350003

基金项目:

福建省农业科学院博士科研启动基金项目 (2011BS-1)

中美合作项目 (2012-2934-01)

公益性行业 (农业) 科研专项 (201003067-05)

详细信息

作者简介:
林雄杰 (1979-), 男, 博士, 主要从事分子植物病理学研究 (E-mail:linxj_019@163.com);刘波 (1957-), 男, 博士, 研究员, 博导, 主要从事农业微生物技术及农业生物药物研究 (E-mail:fzliubo@163.com)

中图分类号: S436.66
计量
- 文章访问数: 107
- HTML全文浏览量: 23
- PDF下载量: 0
- 被引次数: 0
出版历程
- 收稿日期: 2013-09-11
- 刊出日期: 2014-05-18

The Effect of Humidity on Citrus Huanglongbing Thermotherapy in the Field

1. Fruit Research Institute,Fujian Academy of Agricultural Sciences;
2. The United States Department of Agriculture,Animal and Plant Health Inspection Service,Plant Protection and Quarantine,Center for Plant Health Science and Technology;
3. Fujian Academy of Agricultural Sciences

摘要

摘要: 比较加湿处理后热罩和自然条件下热罩对黄龙病的防治效果, 结果表明自然条件处理后30d叶片平均病菌浓度下降了85%以上, 而加湿条件下叶片平均病菌浓度仅下降3.7%, 与自然条件处理间差异达到显著水平;自然条件热罩处理后90d叶片平均病菌浓度下降了97%以上, 而加湿处理病树叶片平均病菌浓度下降了38%, 与自然条件处理间的差异达到极显著水平。对照组叶片平均病菌浓度处理后30d和90d均有略微增加;加湿未热罩处理叶片平均病菌浓度在处理后30d和90d分别增加了4.5倍和8.7倍, 但与对照组无显著差异。本研究结果表明加湿热罩处理病树显著减弱了其杀灭黄龙病菌效果, 同时表明增加土壤湿度对黄龙病菌繁殖可能具有一定的促进作用。
- 柑橘黄龙病 /
- 湿度 /
- 自然热罩 /
- 实时荧光定量PCR /
- 影响
Abstract: Citrus huanglongbing (HLB) is the most destructive disease of citrus, and there is no effective control agent at the present, but control citrus psyllid, excavation of diseased trees.In this paper, we analyzed the effection of humidity on Citrus Huanglongbing thermotherapy in the field.Our results showed that 30 dnature and moist thermotherapy decrease the average concentration of Las in the leaves by 85%and 3.7%.Furthermore, 90 dnature and moist thermotherapy decrease the average concentration of Las in the leaves by 97%and 38%.In the contrast, the average concentrations of Las in leaves under control were slightly increased after 30 dand 90dtreatments;30d and 90 dhumidification without sheeting increased the average concentration of Las in the leaves by 4.5-fold and8.7-fold, but there was no significant difference with the control.Our results suggest that humidification with sheeting decrease the effect on killing Las significantly and increasing the soil moisture will benefit for the breeding of Las.
- citrus huanglongbing /
- humidity /
- thermotherapy /
- real-time PCR /
- effect

HTML全文

参考文献(30)

[1]	LI W B, HARTUNG J S, LEVY L.Evaluation of DNA amplification methods for improved detection of“Candidatus Liberibacter species”associated with citrus huanglongbing[J].Plant Disease, 2007, 91 (1) :51-58.
[2]	REINKING O A.Diseases of economic plants in southern China[J].Philippine Agricultural, 1919, 8:109-135.
[3]	范国成, 刘波, 吴如健等.中国柑橘黄龙病研究30年[J].福建农业学报, 2009, 24 (2) :183-190.
[4]	COLETTA-FILHO H, TARGON M, TAKITA M, et al.First report of the causal agent of Huanglongbing“Candidatus Liberibacter asiaticus”in Brazil[J].Plant Disease, 2004, 88:1382-1382.
[5]	HALBERT S.The discovery of huanglongbing in Florida.Proceedings of the 2nd International Citrus Canker and Huanglongbing Research Workshop[C].2005:7-11.
[6]	BOV J M.Invited review, huanglongbing:a destructive, newly-emerging, century-old disease of citrus[J].Journal of Plant Pathology, 2006, 88 (1) , 7-37.
[7]	华南农学院植保系植病教研组.柑橘木虱传递柑橘黄龙病试验初报[J].广东农业科学, 1977, (6) :50-53.
[8]	赵学源, 蒋元晖, 邱柱石, 等.柑橘黄龙病嫁接传播方法的初步研究[J].植物病理学报, 1982, 12 (2) :53-56.
[9]	MARTINEZ A L, NORA D M, ARMEDILLA A L.Suppression of symptoms of citrus greening disease in the Philippines by treatment with tetracycline antiobiotics[J].Plant Disease, 1970:1007-1009.
[10]	SCHWARZ R E, VUUREN SPV Decrease of fruit greening of sweet orange by trunk injection of tetracyclines[J].The Plant Disease Reporter, 1971:747-750.
[11]	NARIANI T K, RAYCHAUDHURI S P, VISWANATH S M.Response of greening pathogen of citrus to certain tetracycline antibiotics[J].Current Science, 1971:552.
[12]	陈乃荣, 戴月明, 吴泰旭, 等.盐酸四环素等药物对柑橘黄龙病治疗效应[J].植物保护学报, 1981, 8 (3) :163-169.
[13]	ZHANG M Q, DUAN Y P, ZHOU L J, et al.Screening molecules for control of citrus huanglongbing using an optimized regeneration system for‘Candidatus Liberibacter asiaticus’infected periwinkle (Catharanthus roseus) cuttings[J].Phytopathology, 2009:239-245.
[14]	ZHANG M Q, POWELL C A, ZHOU L J, et al.Chemical compounds effective against the citrus Huanglongbing bacterium Candidatus Liberibacter asiaticus'in planta[J].Phytopathology, 2011, 101:1097-1103.
[15]	ZAUDBERGEN M.Hot water treatment for bulbs[J].Roy Hort Soc Daffodil-Tulip Yearbook, 1965:30.
[16]	SANDS D C, FOUREST E, REHMS L D.Dry heat seed treatment for Xanthomonas campestris pv.translucens[C]//7th Int.Conf on Plant Pathogenic Bacteria, Budapest (Klement, Z., Ed.) .Akademiai Kiado, Budapest, 1989:51.
[17]	SHEKHAWAT P S, JAIN M L, CHKRAVARTI B P.Detection and seed transmission of Xanthomonas campestris pv.campestris causing black rot of cabbage and cauliflower and its control by seed treatment[J].Indian Phytopathol1982, 35 (3) , 442-447.
[18]	林孔湘, 郑儒钰.柑橘黄梢 (龙) 病毒和柑橘枝条组织耐热力的初步研究[J].植物病理学报, 1964, 7 (1) :61-65.
[19]	林孔湘, 骆学海.柑橘黄梢 (黄龙) 病热治疗的初步研究[J].植物保护学报, 1965, 4 (2) :169-174.
[20]	SU H J AND CHANG S C.The response of the likubin pathogfn to antibiotics and heat therapy[C]//Calavan, E.C. (ed.) Proc 7th Conf Intern Organ Citrus Virol.Univ Calif Riverside, 1976:27-34.
[21]	骆学海, 罗志达, 唐伟文.柑橘黄龙病热治疗的研究[J].植物保护学报, 1981, 8 (1) :48-51.
[22]	FWWCETT H S, COCHRAN L C.Resistance of citrus tissue and psorosis virus A to heat[J].Phaytopath, 1947, (31) :861.
[23]	PRICE W C, KNORR L C.Kinetics of thermal destruction of citrus tissues in relation to the virus disease problem.Phytopathology, 1956 (46) :657-661.
[24]	LI W B, HARTUNG J S, LEVY L.Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing[J].Journal of Microbiological Methods, 2006:104-115.
[25]	GRONDEAU C, SAMSON R.A Review of Thermotherapy to Free Plant Materials from Pathogens, Especially Seeds from Bacteria[J].Critical Reviews in Plant Sciences, 1994, 13 (1) :57-75.
[26]	BAKAI-GOLAN R.Postharvest heat treatment to control Alternaria tenuis Auct.rot in tomato[J].Phytopathol.Mediterr, 1973 (12) :108-111.
[27]	KAUL J L, MUNJAL R L.Effectiveness of hot water treatments in controlling fungal rots of apple[J].Indian Phytopathol, 1981, 33 (3) 484-485.
[28]	QUAK F.Meristeemcultuur gecombineerd met warmtebehandeting voor bet verkrijgen van virusvrije anjerplanten[J].T Pl-ziekten, 1957, 63:13-14.
[29]	HAKKAART F A, QUAK F.Effect of heat treatment of young plants in freeing chrysanthemums from virus B by means of meristem culture[J].Neth J Pl Path, 1964, 70:154-155.
[30]	QUAK F.Meristeemcultuur van aardbei[J].Ann Rep Inst Phytopath Res, 1964:84.