Identification and Characterization of Escherichia coli Isolates from Salpingitis Duck
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摘要: 为分析表现输卵管炎种鸭体内分离的大肠杆菌的致病性、药敏试验、血清型及毒力基因携带情况, 无菌采集表现输卵管炎的种鸭肝脏, 进行大肠杆菌的分离鉴定, 经易感鸭皮下注射, 测定其致病性;纸片法进行药敏试验;凝集试验确定血清型;PCR法检测毒力基因。结果共分离鉴定大肠杆菌16株, 其中强毒菌株11株。70%以上菌株表现敏感的药物有丁胺卡那霉素、氟苯尼考、头孢唑啉、左氧氟沙星和壮观霉素, 70%以上菌株表现耐受的药物有复方新诺明、卡那霉素、庆大霉素、恩诺沙星和氟哌酸。16株大肠杆菌分属于4个血清型, O78占56.3%, 为主要血清型。毒力基因irp2、fyuA、fimC和papC的检出率分别为68.8%、87.5%、75%和100%。总结说明, 从表现输卵管炎的种鸭体内共分离到16株大肠杆菌, O78是主要的血清型, 首选壮观霉素和氟苯尼考等敏感药物进行治疗。Abstract: To analyze the pathogenicity, medicine sensitive, serotype and virulence associated genes of Escherichia coli (E.coli) strains from salpingitis ducks, the livers of ducks typically showing salpingitis were collected, from which the E.coli was isolated and identified.Pathogenicity of E.coli isolates were determined by subcutaneous injection of susceptible ducks.Serotype of E.coli isolates were detected by agglutination test.Virulence associated genes were detected by PCR.16 strains of duck E.coli were identified, among which there were 11 virulent strains.More than 70%of the strains were sensitive to Amikacin, Florfenicol, Cefazolin, Levofloxacin and Spectinomycin.More than 70% of the strains were resistant to Trimethoprim sulfamethoxazole, Kanamycin, Gentamicin, Enrofloxacin and Ciprofloxacin.These strains were belonged to 4serotypes, among which O78 was the main serotype which accounted for 56.3%.The detection rate of irp2 gene, fyuAgene, fimCgene and papCgene were68.8%, 87.5%, 75%, 100%.16 strains of E.coli were isolated from salpingitis ducks and O78 was the main serotype.Spectinomycin and Florfenicol were the suitable drugs.
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Key words:
- duck /
- salpingitis /
- Escherichia coli /
- serotype /
- medicine sensitive test /
- virulence associated gene
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[1] 程龙飞, 陈红梅, 李宋钰, 等.鸭大肠杆菌强毒株的血清型及生物学特性分析[J].中国生物制品学杂志, 2011, 24 (12) :1437-1441.[2]金文杰, 郑志明, 王倩倩, 等.禽源性大肠杆菌HPI irp2基因序列的扩增及比较[J].扬州大学学报:农业与生命科学版, 2005, 26 (3) :5-7.[3]陈祥, 赵娟, 高崧, 等.我国部分地区猪源大肠杆菌LEE和HPI毒力岛相关基因的检测[J].中国人畜共患病学报, 2006, 22 (1) :33-36.[4]朱善元, 陆辉, 王健.禽源大肠杆菌的分离及其毒力因子的检测[J].微生物学报, 2007, 47 (5) :795-799.[5]于学辉, 程安春, 汪铭书, 等.鸭源致病性大肠杆菌的血清型鉴定及其相关毒力基因分析[J].畜牧兽医学报, 2008, 39 (1) :53-59.[6]王超锋.肉雏鸭传染性浆膜炎与大肠杆菌病混合感染的诊疗[J].河南畜牧兽医, 2013, 34 (4) :50.[7]孙红霞, 王伟涛.鸭传染性法氏囊病与大肠杆菌混合感染的诊治[J].中国动物检疫, 2012, 29 (8) :60-61.[8]周成伟, 胡友章.鸭卵黄性腹膜炎防控的措施[J].养殖技术顾问, 2012, (8) :83.[9]严杜建.鸭源大肠杆菌抗生素敏感性试验研究[J].甘肃畜牧兽医, 2012, 42 (2) :8-11.[10]郑晓何.一起肉雏鸭大肠杆菌病的诊治体会[J].福建畜牧兽医, 2013, 35 (5) :82.[11]CARNIEL E.The Yersinia high-pathogenicity island:an ironuptake island[J].Microbes and Infection, 2001, 3 (7) :561-569.[12]陈文静, 韩先干, 何亮, 等.鸭致病性大肠杆菌的分离鉴定及其生物学特性分析[J].中国动物传染病学报, 2010, 18 (2) :34-40. -
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