二轴生物反应器模拟微重力效应研究以及流体中微载体运动分析

郭林伟; 陈敏; 张翔

doi:10.19303/j.issn.1008-0384.2014.06.016

二轴生物反应器模拟微重力效应研究以及流体中微载体运动分析

doi: 10.19303/j.issn.1008-0384.2014.06.016

1. 福建农林大学,福建福州,350002;
2. 福建省农业科学院,福建福州,350003

基金项目:

福建省自然科学基金项目 (2010J01093)

国家高技术研究发展计划项目 (863计划) (2010AA7035020G)

详细信息

作者简介:
郭林伟 (1989-), 男, 硕士研究生, 研究方向:机械工程陈敏 (1957-), 男, 研究员, 研究方向:受控生态和设施农业 (E-mail:chenmin863@163.com);张翔 (1957-), 男, 教授, 研究方向:现代设计方法及人工智能 (E-mail:zj990302@189.cn);郭林伟 (1989-), 男, 硕士研究生, 研究方向:机械工程陈敏 (1957-), 男, 研究员, 研究方向:受控生态和设施农业 (E-mail:chenmin863@163.com);张翔 (1957-), 男, 教授, 研究方向:现代设计方法及人工智能 (E-mail:zj990302@189.cn);郭林伟 (1989-), 男, 硕士研究生, 研究方向:机械工程陈敏 (1957-), 男, 研究员, 研究方向:受控生态和设施农业 (E-mail:chenmin863@163.com);张翔 (1957-), 男, 教授, 研究方向:现代设计方法及人工智能 (E-mail:zj990302@189.cn)

中图分类号: Q66
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出版历程
- 收稿日期: 2014-02-20
- 刊出日期: 2014-06-18

Research of the Simulated Microgravity Effects in Two-axis Rotating Bioreactor and Micro-carrier Movement in the Fluid

1. Fujian Agriculture and Forestry University;
2. Fujian Academy of Agricultural Sciences

摘要

摘要: 为了解释旋转生物反应器的微重力效应, 研究微重力流体中微载体的运动规律。以有效重力加速度远小于地表重力加速度, 微载体在水溶液中的“近似静止”这2种方法, 验证旋转生物反应器的模拟微重力效应。结合自行研制的旋转生物反应器灌流系统环境, 运用等效截面分析法对微载体运动位移和速度进行分析, 得出在流体环境下运动规律, 并通过计算离心力, 得到细胞瓶内氧合膜的理想实验半径。研究表明微载体由中心按一定规律向外径向运动, 直至触碰到壁面。为了取得较好试验结果, 氧合膜半径为小尺寸参数, 范围在0.373 3⁰.804 2cm。
- 模拟微重力 /
- 灌流系统 /
- 微载体运动
Abstract: For studying the simulated microgravity in rotating bioreactor and the movement of micro-carrier in the microgravity fluid, two methods were applied to verify the effect of simulated microgravity in rotating bioreactor:keeping the effective acceleration of gravity far less than the surface gravity acceleration, or the " approximate static" condition for micro-carrier in the aqueous solution.In the self-developed perfusion system in rotating bioreactor, the motion displacement and speed of micro-carrier were analyzed to find out the movement rules of micro-carrier in the aqueous solution, and the ideal radius of membrane oxygenator in cell bottle was decided by centrifugal force calculating.The results showed that micro-carrier moved from center to outside following a certain rule until touching the wall.To get better test results, the radius of membrane oxygenator was set to be a small parameter between 0.3 733-0.8 042 cm.
- simulated microgravity /
- perfusion system /
- micro-carrier movement

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