Effects of Biogas Slurry Application on Seed Germination and Physiological Effects of Sand Culture Ryegrass
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摘要: 为研究沼液灌溉对砂培黑麦草种子发芽及生理效应的影响, 设置4个梯度沼液灌溉量分别灌溉砂培黑麦草, 分析种子发芽情况和幼苗生理学指标的变化。结果表明:各处理均能正常发芽, 黑麦草种子的发芽率和发芽势随着灌溉沼液量的增加呈现先上升后下降的趋势, 其中以T1试验组的发芽率和发芽势最高, 显著高于T3、T4试验组 (P<0.05) ;沼液灌溉能促进黑麦草幼苗苗高和根长, 试验组T1的促进效果最好;各试验组叶绿素含量和根系活力均随着沼液灌溉量的增加先上升后下降, 且二者的峰值均为T1试验组;不同稀释比沼液试验组间黑麦草幼苗POD酶活性、MDA含量差异不显著 (P>0.05) , SOD、CAT酶活性随沼液灌溉量的增加而增加, 但增加的幅度和趋势存在差异。综合分析认为砂培模式下黑麦草种子能很好地发芽并快速生长成幼苗, 且最适灌溉的沼液与蒸馏水的稀释比为1∶3。Abstract: To study the effect of Biogas slurry application on seed germination and physiological indices of sand culture ryegrass, ryegrass seeds were treated by four different slurry application rates, and then the seed morphological and seedling physiology indices were analyzed.Resultsshowed that treatments have no effect on the germination of sand culture ryegrass.Ryegrass seed germination rate and power were increased first and then decreased with the increase of irrigation biogas slurry application rates.The treatment of T1 biogas slurry application rate with the highest germination rate and germination power which significantly higher than the treatments of T3 and T4biogas slurry application rates (P<0.05.Biogas slurry application could increase the shoot height and the root length of Ryegrass seedling, and the treatment of T1 biogas slurry application rate with the best effect.The chlorophyll content and root activity were increased first and then decreased, and both the peak value of them were the treatments of T1 biogas slurry application rate.The POD and MDA had no significant differences among treatments (P>0.05) .SOD and CAT increased with the increasing of irrigation biogas slurry, but with differences on the rate and trend of increase.Our results suggest that ryegrass seeds could germinate in the sand and the treatment of T1 biogas slurry application rate has the optimum benefit for it.
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Key words:
- biogas slurry /
- ryegrass /
- sand culture /
- germination
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