菌株TLB15发酵培养基的优化及其发酵液的杀虫活性

基金项目:

山东省科技攻关项目 (2010GSF10206)

Optimum Fermentation Medium for Insecticidal Active Substances from Strain TLB15

Shandong Provincial Key Laboratory of Applied Microbiology,China-Japan Friendship Biotechnology Research Center

摘要: 采用正交试验对菌株TLB15的摇瓶发酵培养基进行了优化, 结果表明:优化后的培养基为玉米粉3%、豆粉2%、蔗糖1%、硫酸铵0.1%、磷酸氢二钾0.3%、磷酸二氢钾0.2%、七水硫酸镁0.04%。采用10L小型发酵罐发酵48h后活菌数达4.52×109 cfu·mL-1, 产芽孢率为90%。室内生物测定结果表明, 菌株TLB15发酵液在药后72h对小菜蛾2龄幼虫和甜菜夜蛾2龄幼虫的校正死亡率为66.93%和69.07%。
- 发酵 /
- 培养基优化 /
- 正交设计 /
- 菌株TLB15 /
- 杀虫活性
Abstract: The optimum fermentation culture medium of strain TLB15 was modified through an orthogonal experiment L18 (37) .The results showed that the optimum fermentation culture medium contained 3.0% corm meal, 2.0% soybean powder, 1.0% sugar, 0.1% ammonium sulfate, 0.3% potassium hydrogen phosphate trihydrate, 0.2% potassium dihydrogen phosphate, and 0.04% magnesium sulfate heptahydrate.The number of viable cells was 4.52×109 cfu·mL-1and the rate of spore formation was 90%after 48hs′fermentation.The 72-h adjusted mortality of TLB15 strain fermentation liquid on the larvae of Plutella xylostella and Spodoptera exigua were 66.93%and 69.07%, respectively.
- fermentation /
- medium optimization of /
- orthogonal experiment /
- strain TLB15 /

[1]	赵晓燕, 李纪顺, 周红资, 等.BtR05的鉴定及其对朱砂叶螨的毒力测定[J].福建农林大学学报:自然科学版, 2011, 40 (4) :341-345.
[2]	WHALON M E, WINGERD B A.Bt mode of action and use[J].Arch Insect Biochem Physiol, 2003, 54 (4) :200-211.
[3]	CHATTOPADHYAY A, BHATNAGAR N B, BHATNAGAR R.Bacterial insecticidal toxin[J].Crit Rev Microbiol, 2004, 30 (1) :33-54.
[4]	STURZ A V, CHRISTIE B R, NOWAK J.Bacterial endophytes:potential role in developing sustainable systems of crop production[J].Critical Reviews in Plant Sciences, 2000, 19 (1) :1-30.
[5]	汪澈, 何月秋, 张永庆.枯草芽孢杆菌B9601-Y2抑菌蛋白活性及产生条件的研究[J].植物病理学报, 2005, 35 (1) :30-36.
[6]	赵晓燕, 洪永聪, 郭凯, 等.TL2的鉴定及其对植物病原菌的抑制作用[J].福建农业学报, 2013, 28 (4) :353-356.
[7]	丰贵鹏, 杨丽云.地衣芽孢杆菌发酵培养基的优化[J].安徽农业科学, 2009, 37 (15) :6862-6864.
[8]	吴刚, 尤民生, 赵士熙, 等.苏云金杆菌预处理小菜蛾对有机磷和氨基甲酸酯杀虫剂的增效作用[J].昆虫学报, 2001, 44 (4) :454-460.
[9]	朱玉坤, 尹衍才.微生物农药研究进展[J].生物灾害科学, 2012, 35 (4) :431-434.

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