A New Zoophthora Occidentalis Pathogen of Myzus Persicae in China
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摘要: 从福建省邵武市的桃蚜上分离出1株病原真菌, 通过形态学鉴定为西虫瘟霉Zoophthora occidentalis。其初生分生孢子长椭圆形, 有明显的孢领, 大小为 (23.431.2) μm× (7.810.9) μm, L/D=2.43.8。次生分生孢子与初生分生孢子同形, 大小为 (15.625.4) μm× (7.810.4) μm, L/D=2.464.08。毛管孢子大小为 (20.829.9) μm× (6.010.4) μm, L/D=2.43.6, 柄长36.4104.0μm。菌丝直径6.58.8μm。休眠孢子表面光滑, 无色透明, 直径23.929.9μm。用“孢子浴”方法将该菌对桃蚜接种, 结果显示接种后第4d为死亡高峰期, 最终累计死亡率5.4%95.7%, 因剂量而异。经时间-剂量-死亡率模型分析, 测得接种4d后LD50低于10个孢子·mm-2, 表明该菌株对桃蚜具有较强的毒力。Abstract: A newly discovered fungal pathogen isolated from the green peach aphids, Myzus persicae, in Fujian was identified as Zoophthora occidentalis.Its elongate ovoid primary conidia sized (23.4-31.2) μm× (7.8-10.9) μm (L/D =2.4-3.8) and had a clear spore collar.Secondary conidia liked primary (15.6-25.4) μm× (7.8-10.4) μm (L/D =2.46-4.08) was also oblong in shape (15.6-25.4) μm× (7.8-10.4) μm with an L/D ratio of 2.46-4.08.The size of its capilliconidium was (20.8-29.9) μm× (6.0-10.4) μm (L/D =2.4-3.6) , and the stipe was 36.4-104.0μm long.The diameter of its mycelium was 6.5-8.8μm.Its resting spores were smooth, colorless and transparent with a diameter ranging from 23.9μm to 29.9μm.Most of the infected aphids were killed in the 4th day, and green peach aphids to induce a peaked death rate in 4days and cumulative mortality ranging from 5.4%to 95.7%, which increased with the spore concentration.The resulting data fitted well on a time-dose-mortality model.The LD50 after 4days estimated by the model was less than 10conidias·mm-2, indicating the entomophthoralean fungus to be a potent pathogen for M.persicae.
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Key words:
- Zoophthora occidentalis /
- Myzus persicae /
- bioassay /
- entomophthorales
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