睾丸酮降解菌的分离鉴定及降解特性

白泽方; 潘大仁; 霍煜璟; 李清

doi:10.19303/j.issn.1008-0384.2014.07.019

睾丸酮降解菌的分离鉴定及降解特性

doi: 10.19303/j.issn.1008-0384.2014.07.019

1. 福建农林大学作物科学学院,福建福州,350108;
2. 福建农林大学生命科学学院,福建福州,350108

基金项目:

福建省自然科学基金项目 (2008J0041)

详细信息

作者简介:
白泽方 (1987-), 男, 在读硕士生, 研究方向:农业生物技术 (E-mail:xiaobai2006jay@126.com);潘大仁 (1949-), 男, 博士, 教授, 研究方向:农业生物技术 (E-mail:pdr8598@163.com)

中图分类号: X172
计量
- 文章访问数: 103
- HTML全文浏览量: 30
- PDF下载量: 0
- 被引次数: 0
出版历程
- 收稿日期: 2014-05-03
- 刊出日期: 2014-07-18

Isolation and Characterisation of a Testosterone-Degrading Bacterium

1. College of Crop Science,Fujian Agriculture and Forestry University;
2. College of Life Sciences,Fujian Agriculture and Forestry University

摘要

摘要: 为筛选获得具有降解环境雄激素的菌株, 从福建莆田的土壤中分离得到1株能够高效降解睾丸酮的菌株, 命名为C53-20。该菌经过常规的形态和16SrDNA序列同源性分析, 鉴定为克雷伯氏肺炎菌 (Klebsiella pneumonia) 。该菌在35⁴0℃、pH6.0⁸.0具有较高的降解活性, 其最适值分别为温度37℃和pH6.5。该菌能够以睾丸酮为唯一碳源生长, 12h内对1mmol·L-1睾丸酮的降解率为45%。该菌的筛选获得为进一步研究与睾丸酮降解相关的微生物基因提供资源。
- 降解菌 /
- 睾丸酮 /
- 16SrDNA
Abstract: One bacterium, which has testosterone degradation efficiently, was isolated from the sludge, Fujian and named C53-20.The results of morphological characteristics and the similarity analysis of the 16 SrDNA sequence suggest that it is a Klebsiella pneumonia.In addition, testosterone degradation activity in 35-40℃and pH6.0-8.0range was assayed, and our results suggest that the optimum activity is at 37℃and pH6.5, respectively.Testosterone can be utilized as sole carbon and energy sources and the degradation rate to cholesterol was 45%, with1mmol·L-1 concentrations within 12 hours.Our results suggest that the strain has potential application prospects in environmental remediation of androgen contaminated areas.
- degrading bacteria /
- testosterone /
- 16S rDNA

HTML全文

参考文献(17)

[1]	许澜.雄激素与运动的关系[J].湖北体育科技, 2006, 25 (1) :29-30.
[2]	GALVEZ J I, MORRISON J R.Efficacy of trenbolone acetate in sex inversion of the Blue Tilapia Oreochromis aureus[J].Journal of the World Aquaculture Society, 1996, 27 (4) :483-486.
[3]	游牧, 李朝品.环境污染与生物消长的研究进展[J].环境与健康杂志, 2012, (6) :36.
[4]	吴二社, 张松林, 刘焕萍, 等.农村环境中内分泌干扰物的现状, 危害及处理[J].中国农学通报, 2011, 27 (14) :282-285.
[5]	李艳霞, 韩伟, 林春野, 等.畜禽养殖过程中雌激素的排放及其环境行为[J].生态学报, 2010, 30 (4) :1058-1065.
[6]	韩伟, 李艳霞, 杨明, 等.环境雄激素的危害, 来源与环境行为[J].生态学报, 2010, 30 (6) :1594-1603.
[7]	RAKL V, PRITSCH K, MUNCH J C, et al.Structural and functional diversity of microbial communities from a lake sediment contaminated with trenbolone, an endocrine-disrupting chemical[J].Environmental Pollution, 2005, 137 (2) :345-353.
[8]	KANG I J, YILOTA H, OSHIMA Y, et al.The effects of methyltestosterone on the sexual development and reproduction of adult medaka (Oryzias latipes) [J].Aquatic toxicology, 2008, 87 (1) :37-46.
[9]	鲍丽萍, 易凤仙.现代口服避孕药不良反应研究[J].中国误诊学杂志, 2012, 11 (34) :8350-8351.
[10]	SCHIFFER B, DAXENBERGER A, MEYER K, et al.The fate of trenbolone acetate and melengestrol acetate after application as growth promoters in cattle:environmental studies[J].Environmental health perspectives, 2001, 109 (11) :1145.
[11]	喻子牛, 何绍江, 李阜棣.农业微生物学实验技术[M].北京:中国农业出版社, 1996:140-142.
[12]	纪树兰, 韩磊, 任海燕, 等.一株降解甾体雌激素的克雷伯氏菌ZY3[J].北京工业大学学报, 2007, 32 (11) :1017-1021.
[13]	崔建国, 刘亮, 甘春芳, 等.芳 (杂) 环甾体化合物的合成及生理活性研究[J].化学进展, 2014, 26 (2/3) :320 -333.
[14]	戴艺民, 周以飞, 陈建秋, 等.睾丸酮丛毛单胞菌工程菌构建及稳定性分析[J].农业生物技术学报, 2010, 18 (4) :807-814.
[15]	周玉龙, 李国军, 李阳, 等.奶牛肺炎克雷伯氏菌的分离与鉴定[J].中国奶牛, 2007, (6) :36-38.
[16]	李永峰, 任南琪, 杨传平, 等.16SrDNA测序快速鉴定废水生物处理系统目标细菌[J].哈尔滨工程大学学报, 2006, 26 (6) :806-810.
[17]	艾育芳, 潘大仁, 周以飞, 等.环境中胆固醇降解菌的分离与鉴定[J].中国农学通报, 2011, 27 (18) :139-142.