福建15个茶树品种SSR遗传差异分析与指纹图谱建立

王让剑; 杨军; 孔祥瑞; 郭吉春

doi:10.19303/j.issn.1008-0384.2014.10.008

福建15个茶树品种SSR遗传差异分析与指纹图谱建立

doi: 10.19303/j.issn.1008-0384.2014.10.008

福建省农业科学院茶叶研究所,福建福安,355015

基金项目:

福建省农业科学院科技创新团队建设项目 (CXTD-1-1302)

安徽农业大学农业部茶树生物学与茶叶加工重点实验室开放基金项目 (LTBB20140102)

详细信息

作者简介:
王让剑 (1982-) , 男, 硕士, 助理研究员, 研究方向:茶树种质资源与遗传育种 (E-mail:rangjian.wang@163.com);郭吉春 (1945-) , 男, 研究员, 研究方向:茶树种质资源与遗传育种 (E-mail:faasjcguo@163.com)

中图分类号: S571.1
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出版历程
- 收稿日期: 2014-06-25
- 刊出日期: 2014-10-18

Genetic Variances and DNA Fingerprints of 15 Teas in Fujian

Tea Institute of Fujian Academy of Agricultural Sciences

摘要

摘要: 以本课题组选育的10个审 (鉴) 定品种为材料, 选用福建5个具有代表性品种为参照, 利用SSR分子标记分析其遗传差异并建立分子指纹图谱, 结果表明, 从105对SSR引物中筛选出的6对核心引物具有较高的多态性;15个参试品种遗传相似系数变幅较大 (0.35⁰.91) , 其中8个选育品种及闽北闽南的4个参照种, 相互间的遗传相似系数一般都达到均值以上 (≥0.65) , 高者达0.80⁰.91, 证明其遗传差异小或较小;进行聚类分析, 在相似系数为0.77处, 可将参试材料分为8组, 在相似系数为0.66处, 可将参试材料分为6组;选用3对核心引物组合W08、D07、L11建立15个参试品种的分子指纹图谱, 特征条带大小210⁶30bp, 可将每个品种逐一鉴别, 效果较好。
- 茶树 /
- 选育品种 /
- SSR /
- 遗传差异 /
- 指纹图谱
Abstract: Identification and preservation of tea cultivars plays a crucial role in the production and development of the tea industry in China.This paper presents the genetic variances and molecular fingerprints of 10 tea cultivars selected and cultivated by our research team and 5control cultivars in Fujian by using SSR molecular markers.It aimed to further clarify the genetic differences of these cultivars providing a basis for identification of newly improved tea cultivars and protection of intellectual property rights.In the course, 6core primer pairs with high polymorphism were selected from the 105 published pairs.The genetic similarity coefficient of the 15 tested cultivars varied greatly, ranging from 0.35 to 0.91.It generally reached above average (≥0.65) with high at 0.80-0.91 for the 8tea cultivars and 4control cultivars from South and North Fujian among the tested varieties.Consequently, there was little or no genetic differences among the cultivars.Applying the cluster analysis, all tested materials could be divided into 8groups at the similarity coefficient of 0.77, and 6groups in the similarity coefficient of 0.66.The molecular fingerprints of the tested cultivars were established using the primer pairs combination of W08, D07 and L11.The characteristic stripes obtained were between 210-630 bp, which could conceivably suffice successful identifications of individual cultivars.
- tea[Camellia sinensis (L.) O.Kuntze] /
- cultivars /
- SSR /
- genetic variance /
- fingerprint

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