Molecular Identification for Southern Blight Pathogen of Anoectoch ilusroxburghii(Wall.)Lindl.
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摘要: 采用组织块法对金线莲疑似茎腐病病原菌进行分离, 用致病性试验、病原菌形态学观察、分子生物学方法对该病原菌进行鉴定。结果表明:从收集的金线莲疑似茎腐病病原菌感染植株中分离得到的菌株, 在培养基中产生大量气生菌丝呈棉絮状, 菌丝在培养基中产生紫色色素, 有隔及分枝, 大型分生孢子纺锤形, 小型分生孢子卵形或长圆形。分离的菌株回接后可使金线莲植株产生病害, 对扩增得到的rDNA-ITS序列进行Blast对比分析, 确定该病害为尖孢镰刀菌Fusarium oxysporum Schl.引起的茎腐病。Abstract: A suspected pathogen that caused the stalk rot on Anoectochilus roxburghii was isolated by means of tissue separation.It was subsequently subjected to the pathogenicity tests, morphological observation and molecular biology analysis for positive identification.The massive amount of aerial mycelia of the pathogen were gossypine, separate, branched, and capable of generating apurple pigment.Its macroconidium appeared in the shape of a spindle, and the microconidium in the shape of an egg.By inoculating the isolated strain to A.roxburghii, a pathogenicity to the plant was observed.Based on a blast test of rDNA-ITS sequence, the blight pathogen was identified to be Fusarium oxysporum Schl.
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Key words:
- Anoectochilus roxburghii (Wall.) Lindl. /
- stalk rot /
- pathogens /
- identification
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[1] 福建省中医药研究院.福建药物志:第2卷[M].第1版.福建:福建省科学技术出版社, 1994:492-493. [2] 叶庆荣.珍稀中草药金线莲病虫害综合防治技术[J].中国农村小康科技, 2005, (12) :43-47. [3] 潘国祥.金线莲茎腐病的生态及其防治[D].台北:中兴大学, 1992. [4] 张淑芬.金线莲基腐病与茎腐病之综合防治[D].台北:中兴大学, 1999. [5] 蔡金池, 曾德赐, 谢式钰.金线莲根圈分离木腐菌菌株之生物特性[J].植物病理学公告, 2008, (17) :127. [6] 邵清松, 刘洪波, 赵晓芳, 等.金线莲茎腐病菌的生物学特性及5种杀菌剂对其抑制作用[J].中国中药杂志, 2014.39 (8) :1386-1340. [7] 张兴平, 王鸣.我国西瓜枯萎病菌生理小种分化研究初报[J].中国西瓜甜瓜, 1990, (1) :39-43. [8] 方中达.植病研究方法[M].第3版.北京:中国农业出版社, 1998:122-145. [9] 许志刚.普通植物病理学[M].第4版.北京:高等教育出版社, 2009. [10] NICHOLSON P, PARRY D W.Development and use of a PCR assay to detect Rhizoctonia cerealis, the cause of sharp eyespot of wheat[J].Plant Pathology, 1996, 45:872-873. [11] 肖仲久, 李小霞, 段帅, 等.麻疯树叶枯病菌的鉴定[J].菌物学报, 2010, 29 (5) :874-878. [12] 肖仲久, 李红玫, 蒋选利.草石蚕块茎腐烂病病原菌鉴定[J].植物病理学报, 2011, 41 (2) :210-214. [13] 胡红杏, 吴金平, 郭兰, 等.魔芋白绢病病原菌的分子鉴定及其生物学特性研究[J].湖北农业科学, 2010, 49 (6) :1370-1372. [14] 郭立佳, 彭军, 杨腊英等.大蕉枯萎病病原菌的分离、鉴定和致病性测定[J].热带作物学报, 2013, 34 (1) :105-110. -
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