毛竹内生细菌种群多样性分析

刘芳; 袁宗胜; 张国防; 陈威

doi:10.19303/j.issn.1008-0384.2014.12.015

毛竹内生细菌种群多样性分析

doi: 10.19303/j.issn.1008-0384.2014.12.015

1. 福建农林大学生命科学学院,福建福州,350002;
2. 福建农林大学林学院,福建福州,350002

详细信息

作者简介:
刘芳 (1980-) , 女, 讲师, 研究方向:食用菌和农业微生物 (E-mail:fjliufang@163.com);张国防 (1966-) , 男, 教授, 研究方向:森林培育和经济林栽培 (E-mail:fjzgfzgf@126.com)

中图分类号: S795.7
计量
- 文章访问数: 105
- HTML全文浏览量: 26
- PDF下载量: 0
- 被引次数: 0
出版历程
- 收稿日期: 2014-09-09
- 刊出日期: 2014-12-18

Diversity of Endophytic Bacteria Isolated from Moso Bamboo

1. College of Life science,Fujian Agriculture and Forestry University;
2. College of Forestry,Fujian Agriculture and Forestry University

摘要

摘要: 采用稀释平板法从福建武夷山毛竹根、鞭、杆、叶部组织中分离到27株内生细菌, 利用细菌菌落表征性状和16SrDNA序列对这些菌株进行了多样性分析。结果表明, 分离出27株内生细菌分属于14属18种, 其中以节细菌属Arthrobacter、芽孢杆菌属Bacillus、苍白杆菌属Ochrobactrum和葡萄球菌属Staphylococcus为主要菌群。
- 毛竹 /
- 内生细菌 /
- 多样性
Abstract: Twenty-seven endophytic bacteria were isolated from the roots, rhizome, stems and leaves of moso bamboo by means of grinding separation.A preliminary determination on the diversity of the strains was made based on their phenotypic characteristics as well as the molecular classification using partial 16 SrDNA sequences.The results showed that the isolated bacteria could be classified into 14 genera and 18 species, with Arthrobacter, Bacillus, Ochrobactrumand Staphylococcus being the most predominant.
- moso bamboo /
- endophytic bacteria /
- microbial diversity

HTML全文

参考文献(20)

[1]	胡桂萍, 郑雪芳, 尤民生, 等.植物内生菌的研究进展[J].福建农业学报, 2010, 25 (2) :226-234.
[2]	徐亚军.植物内生菌资源多样性研究进展[J].广东农业科学, 2011, (4) :149-152.
[3]	ROBERT P R, KIERAN G, ASHLEY F, et al.Baeterial endoPhytes:recent developments and application[J].Federation of EuroPean Microbiological Soeieties, 2008, 278:l-9.
[4]	黄瑞虎, 刘会强, 迪丽拜尔·托乎提, 等.植物内生菌及其宿主植物研究概况[J].新疆师范大学学报:自然科学版, 2008, 27 (1) :76-79.
[5]	国辉, 毛志泉, 刘训理.植物与微生物互作的研究进展[J].中国农学通报, 2011, 27 (9) :28-33.
[6]	卢镇岳, 杨新芳, 冯永君.植物内生细菌的分离、分类、定殖与应用[J].生命科学, 2006, 18 (1) :90-94.
[7]	何红, 邱思鑫, 胡方平, 等.植物内生细菌生物学作用研究进展[J].微生物学杂志, 2004, 24 (3) :40-45.
[8]	国家林业局.2005国家森林资源报告[R].北京:中国林业出版社, 2005:12.
[9]	李潞滨, 刘敏, 杨淑贞, 等.毛竹根际可培养微生物种群多样性分析[J].微生物学报, 2008, 48 (6) :772-779.
[10]	齐泽民, 杨万勤.苞箭竹根际土壤微生物数量与酶活性[J].生态学杂, 2006, 25 (11) :1370-1374.
[11]	夏冬亮, 任玉, 李潞滨, 等.毛竹内生细菌分离培养基的选择[J].北京农学院学报, 2009, 24 (1) :15-19.
[12]	DB35/1194-2011.毛竹林丰产培育技术规程[S].
[13]	STURZ A V, CHRISTIE B R, NORWAK J.et al.Potential role in developing sustainable Systems of crop production[J].Critical Reviews in Plant Sciences, 2000, 19 (1) :1-30.
[14]	STURZ, CHRISTIE.EndoPhytic baeteria of red clover as agents of alleloPathic clover-maize syndromes[J].Soil Biology and Biochemistry, 1996, 28, (4/5) :583-588.
[15]	兰海燕, 王长海, 宋荣.棉花内生细菌及其研究进展[J].棉花学报, 2000, 12 (2) :105-108.
[16]	GAGNE S, RIEHARD C, ROUSSEAU H, et al.Xylemresiding baeteria in alfalfa roots[J].CanadianJournal Of Microbiology, 1987, 33:996-1000.
[17]	TRIPATHI, VERMA, CHOWDHURY, et al.Ochrobactrum oryzae sp.nov., an endophytic bacterial species isolated from deep-water rice in India[J].International Journal of Systematic and Evolutionary Microbiology, 2006, 56:1677-1680.
[18]	刘杰, 汪恩涛, 陈文新.豆科植物根瘤内生细菌的发现及其研究进展[J].微生物学报, 2011, 51 (8) :1001-1006.
[19]	PHAM QUANG HUNG, ANNAPURNA K.Isolation and characterization of endophytic bacteria in soybean (Glycnesp.) [J].Omonrice, 2004, 12:92-101.
[20]	芦云, 罗明.哈密瓜内生细菌的分离及拮抗菌的筛选[J].石河子大学学报, 2004, 22:104-109.