摘要:
探讨凝结芽孢杆菌对樱桃谷肉鸭生长性能、血清生化指标、十二指肠内容物消化酶活性和盲肠微生物菌群的影响。选取1日龄樱桃谷公鸭750羽,随机分为5组,每组5个重复,每重复30羽。Ⅰ组为空白对照组,饲喂基础饲粮;Ⅱ组为抗生素对照组,在基础饲粮中添加300 mg·kg-1 10%杆菌肽锌;Ⅲ、Ⅳ、Ⅴ组为试验组,在基础饲粮中分别添加100、200和300mg·kg-1活菌含量为1×109 cfu·g-1的凝结芽孢杆菌制剂,试验期42d。结果表明:1)1~21日龄,Ⅱ、Ⅲ、Ⅳ和Ⅴ组的料重比均显著低于Ⅰ组(P<0.05)。1~42日龄,Ⅳ组平均日增重极显著高于Ⅰ组(P<0.01)、料重比极显著低于Ⅰ组(P<0.01),Ⅴ组日增重显著高于Ⅰ组(P<0.05);2)血清碱性磷酸酶活性、白蛋白含量21日龄各组间均差异不显著(P>0.05),但42日龄Ⅳ组显著高于Ⅰ组(P<0.05);3)Ⅳ和Ⅴ组淀粉酶活性21日龄显著高于Ⅰ组(P<0.05),Ⅲ、Ⅳ和Ⅴ组胰蛋白酶活性42日龄显著高于Ⅰ组(P<0.05);4)盲肠中乳酸杆菌含量21日龄Ⅴ组显著高于Ⅰ和Ⅱ组(P<0.05)、42日龄Ⅳ组显著高于Ⅰ组(P<0.05),大肠杆菌数各阶段Ⅴ组均显著低于Ⅰ组(P<0.05)。可见,在樱桃谷肉鸭配合饲料中添加200~300mg·kg-1的凝结芽孢杆菌可提高其日增重,降低料重比,提高血清中碱性磷酸酶活性和总蛋白含量,同时提高十二指肠内容物消化酶活性,增加盲肠乳酸杆菌含量和降低大肠杆菌含量,改善肠道微生态环境。
Abstract:
In this study,the influences on cherry valley ducks by B.coagulans were investigated at the growth performances,serum biochemical indexes,activities of digestive enzymes in the duodenal contents,and the cecum microbiome.750one-day-old ducklings were allocated into 5groups.Each group had 5replicates,and there were 30 ducklings in each replicate.GroupⅠ was fed with based diet and acted as the negative control.GroupⅡ was supplemented with 300 mg 10%zinc bacitracin per kg of the feed.GroupⅢ,ⅣandⅤ were supplemented with 100,200 and 300mg B.coagulans with the microbial count was 1.0×109 CFU·g-1 per kg of the feed,respectively.The whole experiment lasted for 42 days.The results showed that the feed/weight gain ratio(F/G)in GroupⅡ-Ⅴ were significantly lower than that in GroupⅠ(P<0.05)from Day 1to Day 21.Compared to GroupⅠ,the average daily weight gain(ADG)in GroupⅣ from Day 1to Day 42 were significantly higher(P<0.01),but F/G were significantly lower(P<0.01).The ADG in GroupⅤ was significantly higher than that in GroupⅠ during the same period.The differences of alkaline phosphatase(ALP)activity and albumin(ALB)content among groups were not significantly(P>0.05)on Day 21.But on Day 42,both of them were significantly higher in GroupⅣ than those in GroupⅠ(P<0.05).The amylase activities in GroupⅣandⅤ were significantly higher than that in GroupⅠ(P<0.05)on Day 21.The trypsines in GroupⅢ-Ⅴ were higher than that in GroupⅠ(P<0.05)on Day 42.The amount of Lactobacillus spp.in cecum of ducklings in GroupⅤ was significantly higher than GroupⅠandⅡ(P<0.05)on Day 21.On Day 42,this amount in Group Ⅳ was significantly higher than that in GroupⅠ(P<0.05).On both of Day 21 and 42,the quantity of Escherichia coli in GroupⅤ was significantly higher than that in GroupⅠ(P<0.05).Therefore,we came to a conclusion that the feeding with 200 to 300mg per kg of the feed B.coagulans resulted in the decrease of F/G and the quantity of E.coli in cerum,the increase of ADG,ALP activity,ALB content,trypsin activity,and the amount of Lactobacillus spp.,which helped to improve the environment of microecology.
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