摘要:
为分析20株禽源多杀性巴氏杆菌的药物敏感性及质粒携带特征,采用纸片法测定细菌的药物敏感性,碱裂解法提取细菌的质粒。结果表明16株以上菌株敏感的药物有氧氟沙星、利福平、头孢唑啉、多粘菌素B、强力霉素、左氧氟沙星、氨苄青霉素、恩诺沙星和复方新诺明。13株携带有质粒,携带的质粒为1~7个。细菌耐受药物的种类与是否携带质粒无关。只有携带质粒的菌株对氟苯尼考、多粘菌素B和复方新诺明耐药。说明近期治疗禽霍乱应首选氧氟沙星、利福平、头孢唑啉、多粘菌素B、强力霉素、左氧氟沙星、氨苄青霉素、恩诺沙星和复方新诺明等药物。禽源多杀性巴氏杆菌的质粒可能与对氟苯尼考、多粘菌素B和复方新诺明的耐药有关。
Abstract:
This study aimed to determine the drug-resistance and plasmid-carrying properties of the 20 strains of Pasteurella multocida isolated from avian sources.Their sensitivity to antibiotics was determined by using the diffusion test on paper,and the plasmids extraction by means of the alkaline lysis method.More than 80% of the isolated strains were found sensitive to Ofloxacin,Rifampin,Cefazolin,Polymysin B,Doxycycline,Levofloxacin,Ampicillin,Enrofloxacin and/or compound sulfamethoxazole.The plasmid-carrying rate of the isolates was 65%with 1to 7plasmids found in them.There was no correlation between the drug resistance and plasmid-carrying of the bacteria,as the strains that carried plasmids were shown only resistant to Florfenicol,Polymysin B and compound sulfamethoxazole.It suggested, however,that Ofloxacin, Rifampin, Cefazolin, Polymysin B,Doxycycline,Levofloxacin,Ampicillin,Enrofloxacin and/or compound sulfamethoxazole should be preferential for treating the avian cholera.And,the plasmids found in the P.multocida isolates might be associated with the bacterial resistance to Florfenicol,Polymysin B and compound sulfamethoxazole.
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