微生物发酵床养猪垫料中物质组分GC-MS分析

潘志针; 刘波; 朱慧君; 朱育菁; 张连宝; 陈峥; 郑梅霞; 史怀

doi:10.19303/j.issn.1008-0384.2015.08.017

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微生物发酵床养猪垫料中物质组分GC-MS分析

doi: 10.19303/j.issn.1008-0384.2015.08.017

1. 福建省农业科学院农业生物资源研究所,福建福州,350003;
2. 厦门大学生命科学院,福建厦门,361005

基金项目:

福建省青年科技创新基金项目(2011J05053)

国家“973”前期项目(2011CB111607)

福建省农业科学院青年基金项目(2011QB-20)

国家“948”项目(2011-G25)

详细信息

作者简介:
潘志针(1985-),男,硕士,主要从事生物化学和生物活性物质分析研究;刘波(1957-),男,博士、研究员,主要从事微生物生物技术及农业生物药物研究(E-mail:Fzliubo@163.com)

中图分类号: S828
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- 被引次数: 0
出版历程
- 收稿日期: 2015-05-22
- 刊出日期: 2015-08-18

GC-MS Analysis on Fermentation Pig-barn Bedding Material

1. Institute of Agricultural Biological Resources, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian 350003, China;
2. School of Life Science, Xiamen University, Xiamen, Fujian 361005, China

摘要

摘要: 采用极性从低到高的5种有机溶剂对微生物发酵床养猪中垫料的物质组分进行梯度萃取,物质提取率乙醇>丙酮>乙酸乙酯>氯仿>石油醚。采用气相色谱质谱联用(GC-MS)对不同溶剂层的物质组分进行分析鉴定。研究结果显示,垫料中的物质组分以酯类物质和烃类物质为主,其中石油醚和氯仿对低极性的酯类物质和长链烷烃的提取效果较好,但氯仿层提取的种类更多,对不饱和烃的提取效果要优于石油醚层;乙酸乙酯对烯烃类化合物(包括多环芳烃)有较好的提取效果;丙酮适合全物质提取,对垫料中低极性的烷烃组分到高极性酯组分的提取效果均较好;乙醇溶剂适合极性较高的酯类物质的提取。
- 发酵垫料 /
- 固液萃取 /
- 气相色谱质谱联用
Abstract: Samples from the fermentation beds in pig barns were extracted with petroleum ether,chloroform,ethyl acetate,acetone and ethanol.The quantities extracted by the various solvents were in the order of ethanol>acetone>ethyl acetate>chloroform>petroleum ether.Compositions of these extracts were determined using GC-MS.Esters and hydrocarbons were the main substances in the bedding materials.Petroleum ether and chloroform could more effectively extract the low polar esters and long-chain alkanes,and chloroform produced more compounds and unsaturated hydrocarbons than the others.The ethyl acetate extracts showed more olefin compounds(including polycyclic aromatic hydrocarbons)than those obtained by other 4solvents.Since acetone could effectively extract both alkanes and esters in a wide range of polarity,it was considered the selected solvent for the substance extraction.On the other hand,ethanol could be used to specifically extract high polarity esters.
- fermentation pig-barn bed /
- solid-liquid extraction /
- GC-MS