摘要:
为了解鸭疫里默氏菌SSPA基因的生物学信息,参照GenBank登录的鸭疫里默氏菌SSPA基因序列设计了1对特异性引物,成功克隆6种血清型鸭疫里默氏菌SSPA基因,并利用生物信息学软件DNAstar及在线分析工具SignalP V4.0、TM-HMM Server V.2.0、NetNGly c1.0、YinOYang 1.0和NetPhos 2.0对推导的氨基酸序列进行分析。结果显示:该基因的ORF全长1 692bp,可编码563个氨基酸;氨基酸序列含有1个潜在的N-糖基化位点,1个潜在的O-糖基化位点,以及38个潜在的磷酸化位点(包括12个丝氨酸、9个苏氨酸和17个酪氨酸位点),无跨膜区;该蛋白信号肽切割部位位于第19位的A(丙氨酸)和第20位的Q(谷氨酰胺)之间;6种血清型鸭疫里默氏菌SSPA基因的核苷酸同源性为95.7%~100.0%,氨基酸同源性为98.2%~100.0%。
Abstract:
SSPA genes of six serotypes of Riemerella anatipestifer were amplified with specific primers by PCR.DNA sequencing confirmed that SSPA gene has a ORF(1692bp),encoding 563 amino acid.Molecular analysis of SSPA genes was performed by DNAstar,SignalP V4.0,TM-HMM Server V.2.0,NetNGly c1.0,YinOYang 1.0and NetPhos 2.0.The results showed that the amino acid encoded by the SSPA gene contains one potential N-glycosylation site,one potential O-glycosylation site and 38 potential phosphorylation sites(including 9serine,12 threonine and 17 tyrosine sites),there is no transmembrane area,the signal peptide protein cutting part is between A(ala)in the 19 th and Q(glutamine)in the 20 th.The nucleotide homology of SSPA among different serotypes of Riemerella anatipestifer was 95.7%-100.0%,and amino acid homology was 98.2%-100.0%.
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