芽胞杆菌来源尼日利亚菌素的检测与分析

朱育菁; 刘波; 郑梅霞; 陈峥; 史怀

doi:10.19303/j.issn.1008-0384.2015.10.006

芽胞杆菌来源尼日利亚菌素的检测与分析

doi: 10.19303/j.issn.1008-0384.2015.10.006

福建省农业科学院农业生物资源研究所,福建福州,350003

基金项目:

福建省种业创新项目(2015年)

福建省科技计划项目——省属公益科研院所基本科研专项(2015R1018-1,2015R1018-11)

国家自然科学基金(31370059)

详细信息

作者简介:
朱育菁(1972-),女,博士,研究员,主要从事农业生物药物与生物防治研究(E-mail:zyjingfz@163.com);刘波(1957-),男,博士,研究员,主要从事微生物生物技术及农业生物药物研究(E-mail:fzliubo@163.com)

中图分类号: Q93
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出版历程
- 收稿日期: 2015-08-06
- 刊出日期: 2015-10-18

Analysis on Nigericin from Brevibacillus choshinensis FJAT-10008

Agricultural Bioresources Research Institute，Fujian Academy of Agricultural Sciences，Fuzhou，Fujian 350003，China

摘要

摘要: 对千叶短芽胞杆菌Brevibacillus choshinensis FJAT-10008中尼日利亚菌素成分进行分析与检测。采用高效液相四级杆飞行时间质谱分析千叶短芽胞杆菌B.choshinensis FJAT-10008发酵液中胞外代谢物的成分。利用MassHunter软件,对原始数据进行分子特征提取,通过Metlin代谢物质谱数据库检索比对获得代谢物的信息。在千叶短芽胞杆菌B.choshinensis FJAT-10008发酵液中检测到1 246种代谢物,通过Metlin谱库搜索获得初步鉴定的有206种。其中,发现了具有生物活性的物质尼日利亚菌素Nigericin,其匹配得分达到92.12,占发酵液总代谢物相对含量的1.45%,保留时间为28.701 7min,精确质量数为724.467 9。尼日利亚菌素的发现为千叶短芽胞杆菌来源的多醚类抗生素的开发与利用提供理论依据。
- 千叶短芽胞杆菌 /
- 胞外代谢物 /
- 尼日利亚菌素 /
- 液相四级杆飞行时间质谱
Abstract: Extracellular metabolites of Brevibacillus choshinensis FJAT-10008 were analyzed by liquid chromatographyhybrid quadrupole time-of-flight mass spectrometry and Nigericin from FJAT-10008 was tested. Molecular Feature Extraction(MFE)and database retrieval were applied to access the information of overall metabolites of B.choshinensis.As a result,1246 compounds were detected and 206 of them were identified.The match score of Nigericin was 92.12 and its relative content was 1.45%.The retention time of Nigericin was about 28.7017 min,and its accurate mass was724.4679.The results provided a theory basis for development and utilization of polyether antibiotics fromB.choshinensis.
- liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry /
- Brevibacillus choshinensis /
- extracellular metabolites /
- Nigericin

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