铁皮石斛叶片内生真菌多样性的研究

陈泽斌; 李冰; 王定康; 余磊; 刘佳妮; 徐胜光; 靳松; 任禛

doi:10.19303/j.issn.1008-0384.2015.10.011

铁皮石斛叶片内生真菌多样性的研究

doi: 10.19303/j.issn.1008-0384.2015.10.011

陈泽斌^1,2,
李冰³,
王定康⁴,
余磊¹,
刘佳妮¹,
徐胜光¹,
靳松¹,
任禛¹

1. 昆明学院农学院,云南昆明,650214;
2. 云南省都市特色农业工程技术研究中心,云南昆明,650214;
3. 中国科学院昆明动物研究所,云南昆明,650223;
4. 昆明学院生命科学与技术系,云南昆明,650214

基金项目:

昆明学院人才引进项目(YJL14005)

云南省教育厅科学研究项目(2014Y390)

云南省科技厅应用基础研究计划青年项目(2013FD040)

云南省高校优势特色重点学科(生态学)建设项目资助

国家自然科学基金项目(31460491)

详细信息

作者简介:
陈泽斌(1985-),男,博士,副教授,研究方向:环境微生物(E-mail:zbchenkmu@163.com);李冰(1973-),女,硕士,工程师,研究方向:非临床药物安全性评价(E-mail:libing@mail.kiz.ac.cn)

中图分类号: S567.239;Q93
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- 文章访问数: 137
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- PDF下载量: 0
- 被引次数: 0
出版历程
- 收稿日期: 2015-08-15
- 刊出日期: 2015-10-18

Diversity of Endophytic Fungi in Leaves of Dendrobium candidum

1. Agriculture College, Kunming University, Kunming, Yunnan 650214, China;
2. Engineering Research Center for Characteristics Agriculture of Yunnan Province, Kunming, Yunnan 650214, China;
3. Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunnan 650223, China;
4. Life Science and Technology Department, Kunming University, Kunming, Yunnan 650214, China

摘要

摘要: 为检测铁皮石斛内生真菌种类,采用高通量测序技术测定铁皮石斛内生真菌的ITS2区序列,用Qiime和Mothur等软件整理和统计样品序列数目和操作分类单元(OTUs)数量,分析物种的丰度和Alpha多样性。获得的有效序列和OTU数为41361/33;稀疏曲线表明测序深度充分,OTU的数量接近于饱和。铁皮石斛内生真菌主要分布于裸胞壳属Emericella,构巢裸壳孢菌Emericella nidulans是铁皮石斛内生真菌的优势种群。
- 铁皮石斛 /
- 内生真菌 /
- 高通量测序 /
- 构巢裸壳孢菌
Abstract: To determine the diversity of the endophytic fungi grown on Dendrobium candidum,the ITS2 region from the samples was sequenced using the Illumina MiSeq high-through put sequencing method.The number of sequences and operational taxonomic units(OTUs)of each sample were sorted and calculated using Qiime and Mothur software.The species abundance and distribution,alpha diversity index,and time of species abundance of the fungi were determined.The number of effective sequences was found to be 41 361;and the OTUs was 33.The rarefaction functions indicated an adequate sampling,while the OTU number was close to saturation.It was concluded that the endophytic fungi found in D.candidumbelonged to Emericella,and E.nidulans was the dominant species.
- Dendrobium candidum /
- endophytic fungi /
- high-throughput sequencing /
- Emericella nidulans

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