Correlation Between Prolactin SNPs and Milk Producing of Chinese Hu Sheep——A Preliminary Report
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摘要: 从分子遗传基础入手,研究湖羊催乳素基因在群体中的遗传多态性,试图发现控制绵羊泌乳性状的标记基因,为现代育种提供技术支撑。以41只分娩母羊为研究对象,生产后每周1次,连续8周采用人工挤奶的方法收集泌乳量。用全基因组测序的方法,分析和整理数据找出SNP位点,进行连锁性和单体型分析。最后结合泌乳性状进行相关性分析。结果显示:湖羊56d泌乳期内平均泌乳量为:(0.52±0.07)kg·d-1,乳中各成分含量分别为:乳脂率(3.83±0.90)%、乳蛋白率(5.52±0.63)%、乳糖率(5.50±0.73)%、总固体(15.13±1.61)%。通过测序分析共找到23个SNP位点,结合泌乳性状,共有4项泌乳性状与其中5个SNP位点相关。其中PRL4244位点GG型在第8周泌乳量显著高于TT型(P<0.05);PR1039位点AC型中期乳脂率极显著高于AA型和CC型。PRL1228位点AC型前期乳脂率显著高于AA型和CC型(P<0.05),AC型中期乳脂率极显著高于AA型和CC型(P<0.01),中期总固体AC型显著高于CC型(P<0.05);PR2351位点AG型前期和中期乳脂率显著高于AA型和GG型(P<0.05),AA型中期蛋白率极显著高于AG型和GG型(P<0.01),AG型中期总固体极显著高于AA型和GG型(P<0.01)。PR4591位点CC型前期乳脂率极显著高于TT型(P<0.01),同时CC型中期蛋白率极显著高于TT型和TC型(P<0.01),前期固体率极显著高于TT型。对湖羊41个样品PRL基因测序结果进行分型,共分为25种单体型,有7种单体型频率大于0.02,其中单体型CCATT蛋白率显著高于AAATT型,其他各单体型的乳成分和泌乳量差异均不显著。Abstract: This study aimed to find the genetic markers that control the milk-producing traits of Hu sheep for breeding purpose.Molecular genetic polymorphism of the prolactin gene in the sheep populations was studied.More than 41 parturition ewes were used by separating the ewes from the lambs 20 hours on the 4th day after giving birth,and manually lactated to collect milk from them.On the following day,the milk collection took place at 7am,1∶30pm,and 7pm every day for 8weeks.Each milk sample was analyzed for its chemical composition.After the entire gene sequence was obtained for organizing and analyzing by SPSS 13.0to locate and identify the SNP loci by MEGA6,followed by the linkage and haplotype analysis.Finally,using the least squares method,the correlation between the polymorphisms and the milk-producing traits was established.The results showed that the average milk content of Hu sheep during 56 days was(0.52±0.07)kg·d-1,and the fat,protein,sugar,and total solids contents in the milk were(3.83±0.90)%,(5.52±0.63)%,(5.50±0.73)%,and(15.13±1.61)%,respectively.Based on the genetic and the linkage disequilibrium analyses,4 milk-producing traits were found to significantly correlate with the SNP sites in Hu sheep.At the PRL4244 site,the milk producing amount of the GG type in the 8th week of lactation was significantly higher than the TT genotype(P<0.05);at the PR1039 site,the fat content in the milk during mid-term of the AC-type was significantly higher than AA and CC types;at the PRL1228 site,the fat content of the AC-type was significantly higher than AA and CC types in the early stage(P<0.05),while that of the AC type during mid-term was significantly higher than AA and CC types(P<0.01);the total solids of AC in mid-term were significantly higher than those of the CC genotype(P<0.05),and the total solids of AC type in the mid-term was significantly higher than the CC type(P<0.05);at PR2351 sites,the fat contents of the AG type in the early stage and mid-term were significantly higher than AA and GG genotype(P<0.05),while the protein ratio of the AA type in mid-term was significantly higher than the AG and the GG genotype(P<0.01),and the total solids of the AG type in mid-term were significantly higher than those of the AA or the GG genotype(P <0.01);and,at PR4591 sites,the milk fat content of the CC type in the early stage was significantly higher than the TT genotype(P<0.01),while the protein ratio of the CC type in mid-term was significantly higher than those of the TT and TC types(P<0.01),and the total solids was significantly higher than the TT type in the early stage.There were a total of 25 haplotypes detected,the frequency of 7haplotypes were more than 0.02,and the protein content of CCATT haplotype was significantly higher than that of AAATT haloytpe.No significant diffience were existed in the milk ingredient and milk content among other haplotypes.
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Key words:
- Hu sheep /
- PRL polymorphism /
- milk-producing
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