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苋菜AmaDOPA5-GT基因的启动子克隆、亚细胞定位及表达分析

郑学立 刘生财 谢礼洋 程春振 赖钟雄

郑学立, 刘生财, 谢礼洋, 程春振, 赖钟雄. 苋菜AmaDOPA5-GT基因的启动子克隆、亚细胞定位及表达分析[J]. 福建农业学报, 2015, 30(11): 1064-1070. doi: 10.19303/j.issn.1008-0384.2015.11.008
引用本文: 郑学立, 刘生财, 谢礼洋, 程春振, 赖钟雄. 苋菜AmaDOPA5-GT基因的启动子克隆、亚细胞定位及表达分析[J]. 福建农业学报, 2015, 30(11): 1064-1070. doi: 10.19303/j.issn.1008-0384.2015.11.008
ZHENG Xue-li, LIU Sheng-cai, XIE Li-yang, CHENG Chun-zhen, LAI Zhong-xiong. Promoter-cloning,Protein Subcellular Localization,and Expression Analysis for AmaDOPA5-GT Gene of Amaranth tricolor[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1064-1070. doi: 10.19303/j.issn.1008-0384.2015.11.008
Citation: ZHENG Xue-li, LIU Sheng-cai, XIE Li-yang, CHENG Chun-zhen, LAI Zhong-xiong. Promoter-cloning,Protein Subcellular Localization,and Expression Analysis for AmaDOPA5-GT Gene of Amaranth tricolor[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1064-1070. doi: 10.19303/j.issn.1008-0384.2015.11.008

苋菜AmaDOPA5-GT基因的启动子克隆、亚细胞定位及表达分析

doi: 10.19303/j.issn.1008-0384.2015.11.008
基金项目: 

福建省教育厅a类项目(ja12098)

福建省自然科学基金项目(2013j05045)

高等学校博士学科点专项新教师类科研基金项目(20123515120009)

详细信息
    作者简介:

    郑学立(1975-),男,博士生,助理研究员,主要从事蔬菜育种及园艺植物生物技术研究(E-mail:99345054@qq.com);赖钟雄(1966-),男,研究员,主要从事园艺植物生物技术及分子生物学研究(E-mail:laizx01@163.com)

  • 中图分类号: S636.4

Promoter-cloning,Protein Subcellular Localization,and Expression Analysis for AmaDOPA5-GT Gene of Amaranth tricolor

  • 摘要: 对获得的苋菜环多巴5-糖基转移酶基因(AmaDOPA5-GT)进行生物信息学和蛋白亚细胞定位分析,并利用染色体步移技术克隆该基因的启动子。亚细胞定位结果表明,AmaDOPA5-GT定位于细胞膜。启动子分析结果表明AmaDOPA5-GT启动子中植物光响应元件多达8个,说明光对该基因的表达影响较大。色素含量测定及实时定量PCR结果显示:暗处理抑制苋菜苷合成和AmaDOPA5-GT表达;黄光和绿光抑制苋菜苷合成但对AmaDOPA5-GT表达影响不大;红光显著抑制苋菜苷合成但却显著诱导AmaDOPA5-GT表达;蓝光显著促进苋菜苷合成和AmaDOPA5-GT表达。本研究可为揭示AmaDOPA5-GT基因在苋菜苷的作用奠定基础并为富含甜菜红色素苋菜苷的苋菜育种提供线索。
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出版历程
  • 收稿日期:  2015-09-28
  • 刊出日期:  2015-11-18

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