响应面法优化短短芽胞杆菌FJAT-0809-GLX胞内代谢物质超声波提取工艺

车建美; 刘波; 马桂美; 刘国红; 唐建阳

doi:10.19303/j.issn.1008-0384.2015.11.012

响应面法优化短短芽胞杆菌FJAT-0809-GLX胞内代谢物质超声波提取工艺

doi: 10.19303/j.issn.1008-0384.2015.11.012

1. 福建省农业科学院农业生物资源研究所,福建福州,350003;
2. 福建农林大学生物农药与化学生物学教育部重点实验室,福建福州,350002

基金项目:

福建省农业科学院青年英才计划项目(YC2015-7)

国家自然科学基金项目(31370059)

福建省科技计划项目——省属公益类科研院所基本科研专项(2014R1018-13)

福建省自然科学基金项目(2014J06012)

福建省种业创新与产业化工程项目(2014S1477-1-3)

详细信息

作者简介:
车建美(1977-),女,副研究员,博士,研究方向:微生物生物技术、微生物保鲜(E-mail:chejm2002@163.com);刘波(1957-),男,研究员,博士,研究方向:微生物生物技术与农业生物药物(E-mail:fzliubo@163.com)

中图分类号: Q936
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出版历程
- 收稿日期: 2015-10-08
- 刊出日期: 2015-11-18

Optimization on Ultrasonic Disruption for Extracting Intracellular Metabolites of Brevibacillus brevis FJAT-0809-GLX Using Response Surface Methodology

1. Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2. Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要

摘要: 为提取短短芽胞杆菌FJAT-0809-GLX的胞内代谢物质,利用超声波破碎其细胞,对胞内代谢物质进行优化提取。在超声波输出功率、超声时间以及料液比进行单因素试验的基础上,利用Design-expert进行试验设计,响应面优化得到胞内代谢物质的最佳提取工艺。单因素试验结果表明,当超声时间为30min时,获得的胞内代谢物质较多,胞内代谢物质得率为11mg·g^-1;超声波功率为100 W时,所得胞内代谢物质得率最高,为9.33mg·g^-1;料液比1∶25时所得胞内代谢物质得率最高,为12.22mg·g^-1。进一步通过响应面优化发现,超声时间、超声功率、料液比3个因素对胞内代谢物质得率的影响程度依次为:料液比>超声破碎时间>超声功率。最佳超声波提取方法为:超声功率为318.68 W,超声时间为10min,料液比为1∶25,在此条件下提取的胞内代谢物质得率为12.987 8mg·g^-1。
- 超声波破碎 /
- 短短芽胞杆菌 /
- 响应面法 /
- 提取
Abstract: Ultrasound was applied to rupture the cells of Brevibacilllus brevis FJAT-0809-GLX to facilitate extraction of the intracellular metabolites produced by the bacteria.Effect of ultrasonic power,treatment time,and cell/liquid ratio were studied in a single-factor experiment.Optimum conditions of the extraction were determined using a design-expert program and the response surface method.The single-factor experiment showed that,when the ultrasonic treatment time was 30 min,the greatest recovery rate on the intracellular metabolites,i.e.,11mg·g^-1,could be obtained;when 100 W of ultrasound power was applied,the highest extraction rate of9.33mg·g^-1 was achieved;and,when a cell/liquid ratio was 1∶25implemented,the highest yield of 12.22mg·g^-1 could be reached.Then,the process was optimization by using the response surface method to show that the effects were cell/liquid ratio>ultrasonic application time>ultrasonic power,and the optimal conditions for the ultrasonic disruption were:an application of ultrasonic power at 318.68 W,a total treatment time of 10 min,and a cell/liquid ratio of 1∶25.As a result,ayield of the intracellular metabolites from the extraction at 12.987 8mg·g^-1 was achieved.
- ultrasonic disruption /
- Brevibacillus brevis /
- response surface method /
- metabolite extraction

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参考文献(37)

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响应面法优化短短芽胞杆菌FJAT-0809-GLX胞内代谢物质超声波提取工艺

doi: 10.19303/j.issn.1008-0384.2015.11.012

作者简介:
车建美(1977-),女,副研究员,博士,研究方向:微生物生物技术、微生物保鲜(E-mail:chejm2002@163.com);刘波(1957-),男,研究员,博士,研究方向:微生物生物技术与农业生物药物(E-mail:fzliubo@163.com)

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Optimization on Ultrasonic Disruption for Extracting Intracellular Metabolites of Brevibacillus brevis FJAT-0809-GLX Using Response Surface Methodology

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响应面法优化短短芽胞杆菌FJAT-0809-GLX胞内代谢物质超声波提取工艺

doi: 10.19303/j.issn.1008-0384.2015.11.012

作者简介: 车建美(1977-),女,副研究员,博士,研究方向:微生物生物技术、微生物保鲜(E-mail:chejm2002@163.com);刘波(1957-),男,研究员,博士,研究方向:微生物生物技术与农业生物药物(E-mail:fzliubo@163.com)

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Optimization on Ultrasonic Disruption for Extracting Intracellular Metabolites of Brevibacillus brevis FJAT-0809-GLX Using Response Surface Methodology

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出版历程

目录

作者简介:
车建美(1977-),女,副研究员,博士,研究方向:微生物生物技术、微生物保鲜(E-mail:chejm2002@163.com);刘波(1957-),男,研究员,博士,研究方向:微生物生物技术与农业生物药物(E-mail:fzliubo@163.com)