Sequence Analysis on ORF3 Gene of Porcine Epidemic Diarrhea Virus in Fujian
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摘要: 为探讨福建省猪流行性腹泻病毒（PEDV）ORF3基因遗传变异情况，于2014-2015年从福建省不同地区猪场扩增8株PEDV的ORF3基因，并进行序列比对及遗传进化分析。结果表明：8株分离株均为野毒株；与近年来国内及其周边国家的分离株亲缘关系较近，其核苷酸同源性及推导的氨基酸同源性最高高达100%；与经典株CV777亲缘关系较远，其核苷酸及推导的氨基酸同源性达96.0%~96.9%与92.5%~94.7%；表明近年来福建省PEDV流行株较以往的经典株已经发生了改变。
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关键词:
- 猪流行性腹泻(PED) /
- ORF3基因 /
- 遗传变异
Abstract: To study the ORF3 gene of porcine epidemic diarrhea virus (PEDV) in Fujian, samples were collected from regions in the province during 2014-2015. Eight strains were amplified for sequence alignment and phylogenetic analysis. Genetic evolution analysis showed that the strains were of wild variety and close to those found domestically and in the neighboring countries in recent years with up to 100% homology on the nucleotide and deduced amino acids. But, they were distantly related to the classical strain of CV777, with a nucleotide homology of 96.0%-96.9% and amino acid homology of 92.5%-94.7%. It appeared that genetic alternations had occurred to the PEDV strain currently isolated from the samples collected in the province.-
Key words:
- porcine epidemic diarrhea /
- ORF3 gene /
- genetic variation
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图 1 8株PEDV分离株ORF3基因RT-PCR扩增
注：M为DL2000Marker；1~8分别为：FJ-FQ-01，FJ-FQ-02，FJ-FQ-03，FJ-FQ-04，FJ-FQ-05，FJ-PT-01, FJ-LY-01, FJ-YX-01。
Figure 1. RT-PCR amplification of ORF3 gene from 8 samples
图 2 8株PEDV分离株ORF3基因遗传进化分析
Figure 2. Phylogenetic analysis on 8 strains of PEDV based on ORF3 gene
图 3 8株PEDV分离株ORF3基因核苷酸同源性分析
Figure 3. Homology analysis on nucleotides of ORF3 gene of 8 strains
图 4 8株分离株ORF3基因氨基酸同源性分析
Figure 4. Amino acid homology analysis on nucleotides of ORF3 gene of 8 strains
表 1 参考株相关信息
Table 1. Relevant information on reference strains
GenBank
登录号毒株名称 毒株来源 分离年份 KR873435 KNU-141112-P10 South Korea 2014 KM975739 USA/IA/2013 USA: Iowa 2013 JX560761 SD-M China：Shandong 2012 KC210147 JS-HZ2012 China: Jiangsu 2012 JQ305134 SH1 China: Shanghai 2011 JX088695 GD-B China: Guangdong 2012 KC161186 AH2011 China 2011 KJ777678 LZW China 2014 EU054930 Attenuated DR13 South Korea 2008 AF353511 CV777 Switzerland 1997 Z24733 Br1/87 and CV777 British 1987 
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