Membrane Separation and Concentration of Pigeon Pea Leaf Extract
-
摘要: 在室温下采用微滤-超滤-纳滤多级膜分离纯化木豆叶提取液，分析木豆叶提取液主要有效成分在膜处理前后的保留情况。结果表明，微滤能截留提取液中15.78%的黄酮类物质，超滤能截留提取液中62.52%的黄酮类物质，而纳滤能截留提取液中97.92%的黄酮类物质，溶剂除去率达到了50%，木豆叶提取物中黄酮纯度可达50.13%。超滤浓缩液中牡荆苷含量可达0.069 mg·g-1（HPLC），异牡荆苷含量为4.31 mg·g-1（HPLC）。经过膜处理，显著提高了木豆叶提取液的分离效率，达到了较好的高效浓缩和节能效果。Abstract: Purification of the extract from pigeonpea leaves was studied. Multi-stage membrane separation and concentration using microfiltration, ultrafiltration, and nanofiltration membranes were conducted on the extract at room temperature. Concentrations of the major active ingredients in the filtrates were determined. The results indicated that 15.78% of the total flavonoids in the extract could be retained by the microfiltration membrane, 62.52% by the ultrafiltration membrane, and 97.92% by the nanofiltration membrane. By combining the membranes, the multi-stage filtration could remove as much as 50% of the solvent yielding a concentrated extract containing 50.13% flavonoids. The ultrafitration alone retained a vitexin concentration of 0.069 mg·g-1 and isovitexin of 4.31 mg/g, as determined by HPLC. It appeared that the multi-stage membrane filtration could significantly improve the efficiency of pigeon pea extract separation resulting in a highly concentrated filtrate as well as desired energy conservation.
-
Key words:
- pigeonpea leaves /
- membrane separation /
- flavonoids
-
图 2 微滤、超滤、纳滤过程膜流量变化
Figure 2. Membrane fluxes in microfiltration, ultrafitration, and nanofiltration filtration process
表 1 膜分离过程的总黄酮含量
Table 1. Total flavonoids at various stages of membrane filtration process
测试溶液 浓度/
(μg·mL-1)体积
/mL总黄酮质量
/mg总提取物质量
/g总黄酮纯度
/%总黄酮分布
/%提取原液 1193.96 10200 12178.43 127.00 9.59 100.00 微滤浓缩液(A) 1466.61 1310 1921.26 86.00 2.23 15.78 超滤浓缩液(B) 4225.96 1347 5692.37 26.76 21.27 46.74 纳滤浓缩液(C) 4025.00 1071 4310.78 8.60 50.13 35.40 纳滤透过液(D) - 5100 - - - - 
下载: 导出CSV
-
[1] 唐军, 王文强, 黄春琼.木豆育种及分子生物学研究进展[J].热带农业科学, 2013, 33(8):36-41. http://www.cnki.com.cn/Article/CJFDTOTAL-RDNK201308010.htm [2] 景怡, 景荣琴, 胡天惠.玉米须总黄酮对糖尿病高血脂症大鼠血脂, 血糖水平的影响及抗氧化作用[J].中药药理与临床, 2011, (2):85-86. http://www.cnki.com.cn/Article/CJFDTOTAL-ZYYL201102032.htm [3] 陈华庭, 王虎, 郑菁.黄芪总黄酮提取物对维甲酸所致大鼠骨质疏松的影响[J].中国药师, 2005, 8(11):895-897. http://www.cnki.com.cn/Article/CJFDTOTAL-ZYSG200511001.htm [4] 赵艳敏, 郭燕, 尚冀宁.芪类化合物的分布和生理活性[J].化工科技, 2015, 23(1):77-80. http://www.cnki.com.cn/Article/CJFDTOTAL-JKGH201501021.htm [5] 林励, 谢宁.木豆中牡荆甙与异牡荆甙的分离与鉴定[J].广州中医药大学学报, 1999, 16(1):49-51. http://www.cnki.com.cn/Article/CJFDTOTAL-REST901.013.htm [6] 佟美鸿, 刘威, 付玉杰.等. Hplc法测定木豆中牡荆苷和异牡荆苷含量[J].林产化学与工业, 2008, 28(6):34-38. http://www.cnki.com.cn/Article/CJFDTOTAL-LCHX200806009.htm [7] PICERNO P, MENCHERINI T, LAURO M R, et al.Phenolic Constituents and Antioxidant Properties of Xanthosoma Violaceum Leaves[J]. Journal of agricultural and food chemistry, 2003, 51(22):6423-6428. doi: 10.1021/jf030284h [8] AGNESE A, PEREZ C, CABRERA J. Adesmia Aegiceras:Antimicrobial Activity and Chemical Study[J]. Phytomedicine, 2001, 8(5):389-394. doi: 10.1078/0944-7113-00059 [9] BRAMATI L, AQUILANO F, PIETTA P. Unfermented Rooibos Tea:Quantitative Characterization of Flavonoids by Hplc-Uv and Determination of the Total Antioxidant Activity[J]. Journal of agricultural and food chemistry, 2003, 51(25):7472-7474. doi: 10.1021/jf0347721 [10] VAN HIEN T, HUONG N B, HUNG P M, et al. Radioprotective Effects of Vitexina for Breast Cancer Patients Undergoing Radiotherapy with Cobalt-60[J]. Integrative cancer therapies, 2002, 1(1):38-43. doi: 10.1177/1534735402001001003 [11] 刘明言, 元英进, 朱世斌.中药现代化进展[J].中草药, 2002, 33(3):193-196. http://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO200203000.htm [12] 徐益清, 杨辉, 罗友华.等.中药复方有效成分群在陶瓷膜分离过程中的迁移研究[J].中草药, 2016, (9):17. http://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO201609017.htm [13] 让一峰, 赵伟, 杨瑞金.低胰蛋白酶抑制剂活力的白芸豆Α-淀粉酶抑制剂的工业化制备方法探究[J].食品与发酵工业, 2016, 42(4):1-5. http://www.cnki.com.cn/Article/CJFDTOTAL-SPFX201604001.htm [14] 郑菲艳, 李爱萍, 徐晓俞.木豆叶总黄酮超声提取工艺优化研究[J].福建农业学报, 2013, 28(11):1149-1153. http://www.cnki.com.cn/Article/CJFDTOTAL-FJNX201311015.htm -
点击查看大图
图(3) / 表(1)
计量
- 文章访问数: 1470
- HTML全文浏览量: 183
- PDF下载量: 237
- 被引次数: 0
