Sequence Analysis on gB Gene from Pseudorabies Virus
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摘要: 2011年以来我国多省免疫猪伪狂犬病毒gE基因缺失苗猪场出现变异型猪伪狂犬病毒（PRV）感染，经典猪伪狂犬病毒疫苗（Bartha-k61）对该病无法提供100%有效保护，已有研究发现变异型PRV和经典PRV gE基因存在特征性变异。为明确变异型PRV gB基因特征，本研究对GenBank中登录的部分PRV代表株gB基因进行分析比较。结果表明，我国经典型和变异型PRV在gB蛋白氨基酸编码区第395位、453位、562位和739位存在特征性差异，但不同来源PRV gB基因的核苷酸和氨基酸同源性分别在98.1%~100%和96.1%~100%。从相互之间的遗传进化关系可以看出，PRV遗传进化出现两个大的遗传分支，呈现明显的地域性。新发变异型和经典型PRV在中国PRV遗传进化分支上呈现各自独立进化小分支；我国近年分离的貉源和约克夏梗犬源与新发变异型PRV则处于同一进化小分支。Abstract: Since 2011, variant pseudorabies (PR) had occurred to the Bartha-k61 vaccinated pigs on farms in most provinces in China. The traditional Bartha-k61 vaccine could not prevent those pigs from contracting the viral disease. As a result, substantial financial losses had incurred to the porcine industry. A previous study indicated that there existed distinctive variations between the gE gene of the typical PR virus (PRV) and that of the variant PRV. For comparison, the sequence of the gB gene of the typical PRV was downloaded from GenBank. It was found that at Positions 395,453,562, and 739 they showed different characteristics, and their nucleotide homology was 98.1%-100% and amino acids 96.1%-100%. A phylogenetic analysis suggested that the viruses were from two different branches and differed significantly depending upon their geographic origins. In China, these viruses had different sub-branches. And, the variant PRV shared a same sub-branch in origin with the newly identified Nyctereutes procyonoides and Yorkshire Terrier PR. The information obtained would be valuable for studying biological functions of gB as well as origin-tracing on variant PRV.
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Key words:
- Pseudorabies virus /
- gB gene /
- sequence analysis
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图 1 gB基因核苷酸序列变异区分析
注：仅德国DUL34Pass的核苷酸存在部分核苷酸和其他代表株不同的情况，其核苷酸比Bartha株存在“GCCGTGCGCGCG”插入。Becker株部分序列和我国PRV类似，没有考虑其特性变异。
Figure 1. Regions with variations on nucleotides in gB gene of PRV
图 2 gB基因氨基酸变异区
注：仅德国DUL34Pass的氨基酸存在部分氨基酸和其他代表株不同的情况，其氨基酸比Bartha株存在“AAVR”插入。Becker株部分序列和我国PRV类似，没有考虑其特性变异。
Figure 2. Regions with variations on amino acids gB gene of PRV
表 1 本研究使用的毒株序列信息
Table 1. Information on viruses studied
基因登录号 毒株名称 宿主 年代 国家 文献 KJ717942 Kaplan 野猪 - Hungary [12] JQ809330 DUL34Pass 野猪 - Germany [13] KT983811 Kolchis 家猪 2010 Greece - KT983810 Hercules 家猪 - Greece - GQ325658 Namyangju 家猪 1987 South
Korea[14] JF797217 Bartha 疫苗株 - Hungary [3] JF797219 Becker 家猪 - - [3] AF257079 Ea 家猪 1998 China - KT948052 HN-HK 家猪 2008 China - KT948044 GX-NL 家猪 2007 China - KJ526432 HYYD-China-2008 家猪 2008 China - KP098534 HeN1 家猪 2012 China [10] KP257591 JS-2012 家猪 2012 China [10] KJ789182 TJ 家猪 2012 China [11] KP710980 LY 家猪 2014 China - KM189914 HNB 家猪 2012 China - KT948047 GD-HS2 家猪 2014 China - KT948049 HN-CZ 家猪 2013 China - KM061380 ZJ01 家猪 2012 China - KP895102 Rang 貉 2014 China - KC981239 BJ/YT 约克夏梗犬 2012 China - 注：“-”表示GenBank中没有相关信息。为便于文章基因位置的统一，本研究中的序列位置信息均以Bartha株位置信息为准。 
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表 2 我国经典型和变异型PRV株氨基酸变异区
Table 2. Regions with variations on amino acids in gB genes from typical and variant PRVs
类型 位置 395 453 552 562 570 673 739 842 849 851 经典型 G R S H G G T A E D 变异型 D K S Q G G A A E D 国外 D R G Q S S A T D G
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