Pathogens of Root Rot Disease on Chili Peppers in Fuzhou
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摘要: 为明确福州地区设施辣椒根腐病病原菌种类，于2015-2016年从长乐、福清、闽侯等3地主要设施辣椒栽培区采集辣椒根腐病样本90份，分离到327株病原真菌。根据来源、培养性状以及形态学特征可将这些病原菌分为7种（来源于长乐2种、福清2种、闽侯3种）。通过菌株的致病性测定以及rDNA-ITS序列分析，结果表明来源于长乐的B1、福清的B2和闽侯的B3均具有致病性，并且在系统发育上与茄腐镰孢菌（KT271766.1）同源的置信度达99%。Abstract: Ninety chili pepper samplesinfectedbyroot rot disease were collected from Changle, Fuqing and Minhou counties in Fuzhou during 2015 to 2016 to identify the causitive fungal pathogens.Three hundred and twenty-seven strains were isolated. Based upon their origins, cultural characteristics and morphologies, these isolates were classified into 7 groups including 2 from Changle, 2 from Fuqing, and 3 from Minhou. Through pathogenic and rDNA-ITS sequence analyses, B1, B2 and B3 were determined to be pathogenic and highly homologous with Fusarium solani (KT271766.1) in phylogeny at 99% degree of confidence.
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Key words:
- chili pepper /
- root rot /
- Fusarium solani /
- pathogens /
- identification
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图 4 病原菌的形态特征
注：a为大型分生孢子，b为小型分生孢子和产孢瓶梗。
Figure 4. Morphological characteristics of pathogenic fungus
表 1 辣椒根腐病病原菌分离结果
Table 1. Isolation of root rot pathogens in chili peppers
采集地 分离总块数 长菌块数 菌类编号 分离频率/% 长乐 150 10 A1 6.7 96 B1 64 福清 150 15 A2 10 89 B2 59.3 闽侯 150 9 A3 6 94 B3 62.7 14 C3 9.3 
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表 2 分离物接种辣椒结果
Table 2. Chili peppers infected by separated substance
菌种编号 接种方法 接种株数 发病株数 发病率/% A1 无创伤 75 0 0 创伤 75 0 0 B1 无创伤 75 2 2.7 创伤 75 70 93.3 A2 无创伤 75 0 0 创伤 75 0 0 B2 无创伤 75 1 1.3 创伤 75 63 84 A3 无创伤 75 0 0 创伤 75 0 0 B3 无创伤 75 4 5.3 创伤 75 68 90.7 C3 无创伤 75 0 0 创伤 75 0 0 CK 无创伤 75 0 0 创伤 75 0 0
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