太子参多糖对福瑞鲤部分非特异性免疫功能和抗病力的影响

吴斌

doi:10.19303/j.issn.1008-0384.2017.05.003

太子参多糖对福瑞鲤部分非特异性免疫功能和抗病力的影响

doi: 10.19303/j.issn.1008-0384.2017.05.003

吴斌^{1, 2,}

1.
福建省水产技术推广总站, 福建福州 350002
2.
福建省淡水水产研究所, 福建福州 350002

基金项目:

现代农业产业技术体系建设专项 CARS-46-35

详细信息

作者简介:
吴斌(1978-), 男, 硕士, 高级工程师, 主要从事水产动物病害分子免疫学研究(E-mail:wubinfire@126.com)

中图分类号: S963
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出版历程
- 收稿日期: 2016-12-10
- 修回日期: 2017-03-06
- 刊出日期: 2017-05-01

Effect of Polysaccharides from Radix pseudostellariae on Partial Non-specific Immune Function and Disease Resistance of FFRC Strain Common Carps

WU Bin^{1, 2
,}

1.
Fujian Provincial Fishery Technical Extention Center, Fuzhou, Fujian 350002, China
2.
Freshwater Fisheries Research Institute of Fujian, Fuzhou, Fujian 350002, China

摘要

摘要: 应用分离纯化的太子参多糖伴料投喂福瑞鲤（FFRC Strain Common Carp），分析其对福瑞鲤非特异性免疫功能的调节作用和抗病力的影响。试验共分5个组，第1组投喂基础饲料作为空白对照组，第2~5组分别投喂在基础饲料中按质量比添加0.1%、0.2%、0.3%、0.5%太子参多糖的太子参多糖饲料。连续投喂35 d后，测定各组福瑞鲤血液中血清溶菌酶（LZM）、超氧化物歧化酶（SOD）、酸性磷酸酶（ACP）、碱性磷酸酶（AKP）、补体C₃，以及对嗜水气单胞菌（Js70322NA）的抗病力。结果表明：与空白对照组比较，0.1%太子参多糖试验组的血清LZM含量显著增加（P < 0.05），其他3个太子参多糖试验组血清LZM含量极显著增加（P < 0.01）；各剂量组血清SOD、AKP活性均高于对照组，且剂量等于或高于0.2%太子参多糖试验组的血清SOD差异显著（P < 0.05）；剂量等于或高于0.2%太子参多糖试验组的AKP活性差异极显著（P < 0.01）；各太子参多糖试验组ACP活性、补体C₃含量与对照组相比，均极显著增加（P < 0.01）；福瑞鲤攻毒结果显示，剂量为0.3%和0.5%的太子参多糖试验组免疫保护力最高，数值为54.55%。说明太子参多糖对福瑞鲤的非特异性免疫功能有增强作用，能提高其抗病力，建议在基础饲料在添加0.3%的太子参多糖为最佳。
- 福瑞鲤 /
- 太子参多糖 /
- 非特异性免疫 /
- 抗病力
Abstract: Dietary supplement of polysaccharides from Radix pseudostellariae was studied to determine its effect on the partial non-specific immune function and disease resistance in FFRC strain common carps. The fish were randomly divided into 5 groups, including Group 1 fed with basic diet as control, and Groups 2 to 5 with formulated diet containing 0.1%, 0.2%, 0.3%, and 0.5% of the polysaccharides, respectively. After feeding for 35 d, the content of lysozyme (LZM) and the activities of superoxide dismutase (SOD), acid phosphatase (ACP), and alkaline phosphatase (AKP), as well as the content of serum complement C₃ in the blood of the carps were analyzed. A challenge test was conducted by infecting the carps with Aeromonas hydrophila. The results showed that LZM in Group 2 increased significantly (P < 0.05), while the other treatment groups increased extremely significantly (P < 0.01), as compared to control. The SOD and AKP activities in the treated fish were higher than those of control. SOD in the carps of Group 3, 4 and 5 were significantly higher (P < 0.05), and AKP extremely significantly greater (P < 0.01), ACP and serum complement C₃ extremely significantly higher (P < 0.01) than those of control. Fish in Group 4 and 5 showed significantly lower mortality against the A. hydrophila infection than control, with an averaged relative survival rate of 54.55%. It appeared that the polysaccharides could enhance the non-specific immunity and disease resistance of the carps, and that the best result achieved with a supplementation of 3 000 mg·kg^-1 in the diet.
- FFRC strain common carp /
- Radix pseudostellariae polysaccharides /
- non-specific immune /
- disease resistance

HTML全文

图 1 太子参多糖对福瑞鲤免疫保护率

Figure 1. Relative protective rate of R. pseudostellariae polysaccharides for FFRC strain common carps

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图 2 嗜水气单胞菌攻毒后福瑞鲤累计死亡数

注：a、b、c、d、e分别代表第1、2、3、4、5组。

Figure 2. Cumulative mortality of immunized FFRC strain common carps against live A. hydrophila

下载: 全尺寸图片幻灯片

表 1 福瑞鲤血清溶菌酶含量

Table 1. LZM in serum of FFRC strain common carps

表 2 福瑞鲤血清SOD活性

Table 2. Serum SOD activity of FFRC strain common carps

表 3 福瑞鲤血清ACP和AKP活性

Table 3. Serum ACP and AKP activities of FFRC strain common carps

表 4 福瑞鲤血清补体C₃含量测定

Table 4. Serum complement C₃ content of FFRC strain common carps

参考文献(14)

[1]	DALMO R A, INGEBRIGTSEN K, BOGWALD J.Non-specific defence mechanisms in fish, with particular reference to the reticuloendothelial system(REB)[J]. Journal of fish diseases, 1997, 20:241-273. doi: 10.1046/j.1365-2761.1997.00302.x
[2]	ELLSAESSER C F, BLY J E, CLEM L W.Phylogeny of lymphocyte heterogeneity:The thymus of the channel catfish[J].Developmental and comparative immunology, 1998, 12:787-799. https://www.ncbi.nlm.nih.gov/pubmed/3264795
[3]	张永安, 孙宝剑, 聂品.鱼类免疫组织和细胞的研究概况[J].水生生物学报, 2000, 24(6):648-654. http://www.cnki.com.cn/Article/CJFDTOTAL-SSWX200006012.htm
[4]	白东清, 吴旋, 杨广, 等.枸杞多糖对黄颡鱼免疫细胞活性的影响[J].水产科技情报, 2011, 38(1):6-9. http://www.cnki.com.cn/Article/CJFDTOTAL-SCKJ201101003.htm
[5]	胡兵, 刘军, 侯永清, 等.黄芪多糖对异育银鲫非特异性免疫力的影响[J].水利渔业, 2008, 28(3):108-111. http://www.cnki.com.cn/Article/CJFDTOTAL-SCAN200803046.htm
[6]	王艳, 吴志新, 庞素风, 等.果寡糖对银鲫非特异性免疫功能的影响[J].水生生物学报, 2008, 32(4):488-492. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-OGSB200711001145.htm
[7]	覃川杰, 汪成竹, 陈晓辉, 等.茯苓多糖对中华鳖非特异性免疫功能的免疫调节作用[J].淡水渔业, 2006, 36(6):40-43. http://www.cnki.com.cn/Article/CJFDTOTAL-DSYY200606010.htm
[8]	向桢, 郑曙明, 杨进才, 等.复方乌梅对鲤鱼非特异性免疫的影响[J].饲料工业, 2012, 33(18):25-30. http://www.cnki.com.cn/Article/CJFDTOTAL-FEED201218009.htm
[9]	刘铁铮, 张小葵, 王桂春, 等.不同中药组方对鲤鱼生长、非特异性免疫及淋巴细胞转化的影响[J].水产科学, 2011, 30(8):445-450. http://www.cnki.com.cn/Article/CJFDTOTAL-CHAN201108004.htm
[10]	苏岭. 三种中药复方对鲤鱼生长及免疫性能影响的研究[D]. 上海: 上海海洋大学, 2011: 15-26. http://www.shangxueba.com/lunwen/v1096561.html
[11]	聂国兴, 明红, 王俊丽, 等.木聚糖酶对尼罗罗非鱼部分非特异性免疫指标及生长的影响[J].中国水产科学, 2007, 14(7):66-71. http://www.cnki.com.cn/Article/CJFDTOTAL-HTYY200504004.htm
[12]	谢忠毅. 太子参多糖对鲤鱼肠道黏液细胞分布及数量的影响[D]. 福州: 福建农林大学, 2013: 6-7.
[13]	刘云, 孔伟丽, 姜国良.2种免疫多糖对刺参组织主要免疫酶活性的影响[J].中国水产科学, 2008, 15(5):787-793. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSCK200805011.htm
[14]	殷海成.酵母免疫多糖对黄河鲤鱼生长和非特异性免疫力的影响[J].粮食与饲料工业, 2009, (6):38-45. http://www.cnki.com.cn/Article/CJFDTOTAL-LSYS200906017.htm