Genetic Diversity of Sugarcane Cultivars Bred at Fujian Academy of Agricultural Sciences
-
摘要: 对福建省农业科学院亚热带农业研究所自育品种（系）和常规对照种进行SCoT分析，探讨甘蔗品种间的亲缘关系。结果显示：15条SCoT引物对23份供试材料扩增总共得到122个可分辨的清晰条带，平均每条引物产生8.1个条带，其中多态性条带69条，多态性比率为56.56%；聚类分析后发现23份甘蔗品种存在较小的遗传差异，相似系数为0.77~0.98，说明育种亲本来源相近，重复利用多，遗传多样性不丰富。Abstract: Molecular marker technique of SCoT was applied to analyze the genetic diversity of the sugarcane varieties (or lines) bred at Fujian Academy of Agricultural Sciences, along with the conventional cultivars for comparison. Establishment of the genetic relationship among the germplasms provided the needed reference for breeding and identification of the existing resource. Fifteen SCoT primers were selected to amplify the genome DNAs of 23 test materials. A total of 122 loci, with 56.56% of them polymorphic, were obtained averaging 8.1 bands detected by each primer. A clustering analysis revealed a minute genetic difference existed among 23 sugarcane varieties with a similarity coefficient ranging from 0.77 to 0.98, indicating a close relationship in origins of the breeding parents, duplicated applications, and low genetic diversities among the cultivars.
-
Key words:
- sugar cane /
- bred varieties /
- SCoT /
- genetic diversity
-
图 1 23份甘蔗品种SCoT标记多样性聚类
Figure 1. Cluster analysis dendrogram of 23 sugarcane varieties based on SCoT analysis
表 1 供试材料及其亲本来源
Table 1. Sugarcane varieties tested
编号 品种(系) 亲本 1 闽糖86-2121 Q61×Cp49-50 2 闽糖86-05 Co1001×崖城73-152 3 闽糖92-649 ROC1×Co1001 4 闽糖92-142 桂糖57-624×湛蔗74-141 5 闽糖92-505 Co1001×Cp73-1547 6 闽糖93-246 崖城84-153×Cp49-50 7 闽糖95-261 ROC1×Cp72-1210 8 闽糖96-1409 Co1001×崖城73-226 9 闽糖96-6016 闽糖70-611×Co1001 10 闽糖01-77 ROC20×崖城84-153 11 闽糖02-205 崖城90-3×ROC10 12 闽糖04-250 ROC10×崖城84-153 13 闽糖06-1405 闽糖92-649×ROC10 14 闽糖07-804 ROC10×Co1001 15 闽糖07-648 闽糖92-649×ROC25 16 闽糖07-2005 崖城73-512×ROC22 17 闽糖08-410 ROC10×Cp67-412 18 闽糖09-104 ROC10×Cp67-412 19 闽糖11-610 粤农73-204×Cp72-1210 20 闽糖12-1903 粤糖93-159×Cp72-1210 21 闽糖12-1404 Cp92-1666×闽糖92-649 
下载: 导出CSV
表 2 试验中使用的SCoT分子标记引物相关信息
Table 2. Information on SCoT primers applied for experimentation
引物名称 引物序列(5′-3′) GC含量 扩增条带数 多态性条带数 SCoT1 CAACAATGGCTACCACCA 50 8 2 SCoT3 CAACAATGGCTACCACCG 56 7 5 SCoT4 CAACAATGGCTACCACCT 50 8 6 SCoT5 CAACAATGGCTACCACGA 50 8 6 SCoT11 AAGCAATGGCTACCACCA 50 7 3 SCoT15 ACGACATGGCGACCGCGA 67 10 4 SCoT19 ACCATGGCTACCACCGGC 67 11 4 SCoT31 CCATGGCTACCACCGCCT 67 9 7 SCoT33 CCATGGCTACCACCGCAG 67 7 2 SCoT36 GCAACAATGGCTACCACC 56 8 5 SCoT38 AAGCAATGGCTACCACCG 56 8 5 SCoT39 ACGACATGGCGACCAGCG 67 9 6 SCoT40 ACGACATGGCGACCACGT 62 8 5 SCoT44 GCAACAATGGCTACCACG 56 8 6 SCoT46 CCATGGCTACCACCGGCA 67 6 3
下载: 导出CSV
-
[1] 曾华宗, 郑成木, 朱稳, 等.甘蔗种质间亲缘关系及特异标记的RAPD分析[J].植物遗传资源学报, 2003, 4(2):99-103. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zwyczyxb200302003 [2] 肖关丽, 李富生, 杨清辉, 等. RAPD分子标记在甘蔗杂种鉴定中的应用研究[J].西南农业大学学报, 2003, 25(3):207-209. https://www.wenkuxiazai.com/doc/5d19b6be0066f5335b812157.html [3] 余爱丽, 张木清, 陈如凯, 等. ISSR分子标记在甘蔗及其近缘属分类上的应用[J].福建农林大学学报(自然科学版), 2002, 31(4):489-494. http://industry.wanfangdata.com.cn/dl/Detail/Periodical?id=Periodical_fjnydxxb200204019 [4] 王英, 陈守俊, 朱相成, 等. 80份甘蔗种质RAMP标记遗传多样性分析[J].植物遗传资源学报, 2011, 12(4):525-532. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWYC201104008.htm [5] Da SILVA J A D, SORRELLS M E, BURNQUIST W L, et al. RFLP linkage map and genome analysis of Saccharum spontaneum[J]. Genome, 1993, 36(4):782-791. doi: 10.1139/g93-103 [6] COLLARD B C Y, MACKILL D J. Start codon targeted(SCoT) polymorphism:A simple, novel DNA marker technique for generating gene-targeted markers in plants[J]. Plant Molecular Biology Reporter, 2009, 27:86-93. doi: 10.1007/s11105-008-0060-5 [7] 陈辉, 范源洪, 蔡青. RAPD分子标记技术及其在甘蔗育种上的应用[J].中国糖料, 2004, 26(1):46-48. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgtl200401015 [8] 朱岩芳, 祝水金, 李永平, 等. ISSR分子标记技术在植物种质资源研究中的应用[J].种子, 2010, 29(2):55-59. http://mall.cnki.net/magazine/Article/SJKX200605015.htm [9] 苏亚春, 凌辉, 王恒波, 等.甘蔗SCoT-PCR反应体系优化与多态性引物筛选及应用[J].应用与环境生物学报, 2012, 18(5):810-818. http://www.cibj.com/oa/pdfdow.aspx?Sid=201109005 [10] 蔡元保, 杨祥燕, 陈豪军, 等. SRAP结合SCoT标记分析番木瓜种质的遗传多样性[J].植物遗传资源学报, 2014, 15(2):68-74. doi: 10.13430/j.cnki.jpgr.2014.02.010.html [11] 陈虎, 何新华, 罗聪, 等.龙眼24个品种的SCoT遗传多样性分析[J].园艺学报, 2010, 37(10):1651-1654. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yyxb201010014 [12] 齐永芳, 劳芳业, 张垂明, 等.中美重要甘蔗种质SSR遗传多样性比较[J].热带作物学报, 2011, 32(1):99-104. http://www.cqvip.com/QK/95551X/201101/37233174.html [13] 劳芳业, 刘睿, 何惠怡, 等.广东甘蔗品种遗传多样性的AFLP分析[J].热带亚热带植物学报, 2008, 17(1):43-48. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=rdyrdzwxb200901008 [14] WACLAWOVSKY A J, SATO P M, LEMBKE C G, et al. Sugarcane for bioenergy production:an assessment of yield and regulation of sucrose content[J].Plant Biotechnol J, 2010(8):263-276. http://cn.bing.com/academic/profile?id=9611ff70487976a6430a9d5df716ffcf&encoded=0&v=paper_preview&mkt=zh-cn [15] JACKSON P A.Breeding for improved sugar content in sugarcane[J]. Field Crops Research, 2005, 92(2):277-290. http://cn.bing.com/academic/profile?id=2eaad26bd010e4278f6f5e6630b53230&encoded=0&v=paper_preview&mkt=zh-cn -
点击查看大图
图(1) / 表(2)
计量
- 文章访问数: 1195
- HTML全文浏览量: 239
- PDF下载量: 10
- 被引次数: 0
