Study on Antigen Correlation of Different PRV Strains
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摘要: 为了解PRV不同毒株之间的抗原性差异，评价弱毒FB株预防新型PRV的潜力，以4株PRV（FA、FB、Bartha-K61和FJ2012）毒株及其高免血清为材料，应用交叉中和试验测定PRV不同毒株的抗原性差异，通过FB免疫猪攻毒试验评价弱毒FB株对新型PRV的保护潜力。结果表明：中和抗体测定表明兔抗FB株、FA株、Bartha-K61株和FJ2012株高免血清的中和效价分别为1:54、1:91、1:91和1:215。交叉中和试验显示FB株与FA株、FB株与FJ2012株、FA株与FJ2012株的抗原相关性（R值）均大于0.8，Bartha-K61株与FA株、FB株、FJ2012株的R值均小于0.4，表明FB株与FJ2012株的抗原相关性高于Bartha-K61株与FJ2012株。仔猪免疫攻毒试验显示FB免疫猪能抵抗新型PRV（FJ2012株）的攻毒，保护率100%。提示PRV弱毒FB株有望成为预防新型猪伪狂犬病毒（FJ2012株）的疫苗候选株。
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关键词:
- 伪狂犬病病毒(PRV) /
- FA株 /
- FB株 /
- Bartha-K61株 /
- FJ2012株 /
- 抗原相关性 /
- 免疫保护
Abstract: Antigenicity difference among strains of pseudorabies virus (PRV) and potential of developing a vaccine to prevent new PRV from occurring were studied. Four PRV strains (i.e., PRV FB, FA, Bartha-K61 and prevalent PRV-FJ2012) and hyperimmune serums were used for the analysis by a cross neutralization test. The possibility of using PRV-FB to develop a vaccine was evaluated by a challenge test. The neutralization titers of hyperimmune serum for rabbit anti-FB, anti-FA, anti-Bartha-K61 and anti-FJ2012 were 1:54, 1:91, 1:91, and 1:215, respectively. The correlation coefficients between FB and FA, FB and FJ2012, and FA and FJ2012 were all greater than 0.8. However, those between Bartha-K61 and FA, Bartha-K61 and FB, and Bartha-K61 and FJ2012 were lower than 0.4. The results indicated that the relationship between the antigens of FB and FJ2012 was closer than that of Bartha-K61 and FJ2012. The viral challenge test showed that FB could prevent PRV-FJ2012 at a rate of 100%.These conclusion indicated FB strain was a vaccine candidate strain to prevent the new PRV strain(FJ2012 strain).-
Key words:
- pseudorabies virus (PRV) /
- FA strain /
- FB strain /
- Bartha-K61 strain /
- FJ2012 strain /
- antigen correlation /
- immune protection
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表 1 4株PRV交叉中和抗体效价及其抗原相关性R值
Table 1. VN antibodies and correlation coefficients on antigens of 4 strains of PRV
病毒株 高免血清* R值 FAab FBab Bartha-K61ab FJ2012ab FA FB Bartha-K61 FJ2012 FA 6.5 5.5 5.25 6.5 1 FB 6.25 5.75 5.0 6.5 0.84 1 Bartha-K61 6.25 5.25 7.75 5.5 0.39 0.32 1 FJ2012 6.25 5.5 5.5 6.5 0.92 0.92 0.32 1 *注：表中中和抗体数据为-lg2值。 
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表 2 仔猪FB株免疫后对新型PRV FJ 2012株的攻毒保护结果
Table 2. Viral challenge test of FB on preventing PRV FJ2012 infection
组别 数量/头 免疫剂量 攻毒日龄/d 攻毒剂量 发病率/% 死亡率/% 免疫攻毒组 5 105.5TCID50 40 106.5TCID50 0 0 对照攻毒组 5 0 40 106.5TCID50 100 40 健康对照组 3 0 40 0 0 0
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