Studies on Antioxidant Activity of Artificial Culture and Wild Chlorophyllum molybdites
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摘要: 利用秀丽线虫模型及DPPH·清除试验对铅绿褶菇进行体内和体外抗氧化活性研究。结果显示，铅绿褶菇发酵液可明显提升秀丽线虫的抗氧化能力，0.5 mg·mL-1时，可显著提高秀丽线虫的平均寿命，比空白对照组提高了70%，野生未开伞子实体的抗氧化能力较开伞子实体强；未开伞子实体水提液0.5～1.0 mg·mL-1处理的秀丽线虫平均寿命分别为4.85 d和5.06 d，显著高于空白对照组。发酵液对DPPH·的清除率最高，达98.14%，其次是开伞子实体水提液92.86%，未开伞子实体水提液为85.71%。表明铅绿褶菇可成为抗氧化物开发的潜在生物材料。Abstract: Lifespan of Caenorhabditis elegans in vivo, along with the DPPH radical scavenging test in vitro, was used to evaluate the anti-oxidative activity of Chlorophyllum molybdites. The results showed that the C. molybdites fermentation broth increased the resistance of the nematodes to oxidative stress. At 0.5 mg·mL-1, the broth extended the mean lifespan of the paraquat-treated C. elegans 70% longer as compared to that of CK. The anti-oxidative activity of the water extract of immature fruitbodies was greater than that of the mature fruitbodies of C. molybdites. When treated with the water extract of immature mushrooms at 0.5 and 1.0 mg·mL-1, the mean lifespans of the nematodes were 4.85 d and 5.06 d, respectively, which were significantly longer than that of CK. The DPPH scavenging rate of the fermentation broth was the highest, at 98.14%, followed by that of the water extract of mature fruitbodies, at 92.86%, and that of the water extract of immature fruitbodies, at 85.71%. The findings suggested that C. molybdites could potentially be a new source for producing antioxidant from a biomaterial.
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Key words:
- Chlorophyllum molybdites /
- antioxidant /
- DPPH /
- Caenorhabditis elegans
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图 2 铅绿褶菇野生未开伞子实体对秀丽线虫抗氧化能力的影响
Figure 2. Life and mean lifespan of paraquat-treated nematodes pretreated with or without of immature wild C. molybdites fruitbody extract
图 3 铅绿褶菇野生开伞子实体对秀丽线虫抗氧化能力的影响
Figure 3. Life and mean lifespan of paraquat-treated nematodes pretreated with or without of mature wild C. molybdites fruitbody extract
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