Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang
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摘要:
目的 检测浙江省40份水稻栽培品种和6份稻瘟病菌生理小种鉴别品种中6个抗性基因分布情况，同时鉴定品种苗瘟抗性水平，探讨携带抗性基因和抗性水平相关性。 方法 利用功能性分子标记检测品种中Pi1、Pi9/Piz、Pi2/Pizt、Pikh、Pikm、Pita，6个稻瘟病抗性基因的分布情况。用2015-2017年田间采集到的141个稻瘟菌菌株鉴定46个水稻品种苗瘟抗性水平。 结果 6个抗性基因在浙江省栽培品种中分布频率不同，其中Pita基因分布最广，占47.83%；其次是Pikh，占41.30%；抗性基因Pi2/Pizt和Pikm在栽培品种中分布频率相等，均为34.78%；抗性基因Pi9/Piz在栽培品种中分布较少，仅有21.74%；抗性基因Pi1仅在稻瘟病菌生理小种鉴别品种TTP中检测到。抗性水平鉴定结果显示22个品种的抗性频率在60%以上，两个品种的抗性频率达到80%以上。 结论 浙江40份水稻栽培品种和6份稻瘟病菌生理小种鉴别品种携带抗性基因和其抗性水平部分相关。 Abstract:Objective The distribution of 6 resistance genes in 40 cultivated rice varieties in Zhejiang (CRZ) and 6 physiological race identification (PRI) cultivars of Magnaporthe grisea as well as the blast resistance of the rice seedlings were studied. Method The distribution of 6 resistance genes, i.e., Pi1, Pi9/Piz, Pi2/Pizt, Pikh, Pikm and Pita, in the rice varieties was obtained using functional markers. The resistance to M. grisea of all 46 rice varieties was determined based on the 141 strains collected in the field from 2015 to 2017. Result Among the resistance genes, Pita had the highest distribution frequency at 47.83% followed by Pikh at 41.30%; Pi2/Pizt and Pikm, same at 34.78%; Pi9/Piz, low at 21.74%; and Pi1 found only in TTP, one of PRI cultivars. In all, there were 22 rice varieties that showed a resistance rate beyond 60%, and 2 varieties more than 80%. Conclusion The resistance genes carried by the 40 CRZ and the 6 PRI cultivars partially correlated with the resistance level exhibited by the cultivars. -
Key words:
- rice /
- rice leaf blast /
- molecular marker /
- resistance gene
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图 1 6个稻瘟病基因抗感性分子标记检测
注：M为DL2000，A为Pi1基因抗性分子标记检测，CK为IRBL1-CL; B为Pi9/Piz基因抗感分子标记检测，CK为IRBL9-W; C为Pi2/Pizt基因抗性分子标记检测，CK为IRBLz5-CA; D为Pikh基因抗感分子标记检测，CK为IRBLkh-K3; E为Pikm基因抗感分子标记检测，CK为IRBLkm-Ts; F1为 Pita基因抗性分子标记检测，F2为 Pita基因感性分子标记检测，CK为IRBLta2-Pi.第二泳道为阴性对照丽江新团黑谷.泳道1~12为部分浙江省主栽品种材料，依次是Ⅱ优650、Y两优2号、宁81、Y两优1号、Y两优689、春优84、丰两优香1号、嘉58、EX119、浙粳86、内五优8015、钱优0508。
Figure 1. Detection of 6 rice blast resistance genes by specific molecular markers
Note:M: DL2000;A: Identification of Pi1 with resistance molecular markers, CK: IRBL1-CL; B: Identification of Pi9/Piz with resistance and susceptible molecular markers, CK: IRBL9-W; C: Identification of Pi2/Pizt with resistance and susceptible molecular markers, CK: IRBLz5-CA; D: Identification of Pikh with resistance and susceptible molecular markers, CK: IRBLkh-K3; E: Identification of Pikm with resistance molecular markers, CK: IRBLkm-Ts; F1: Identification of Pita with resistance molecular markers; F2: Identification of Pita with susceptibility molecular markers, CK: IRBLta2-Pi. Second line is negative control, LTH. Line 1-12: some main varieties in Zhejiang, i.e., Ⅱ you 650, Y Liangyou 2, Ning 81, Y Liangyou 1, Y Liangyou 689, Chunyou 84, Feng Liangyou 1, Jia 58, EX119, Zhegeng 86, Neiwuyou 8015, Qianyou 0508.
图 2 6个抗稻瘟病基因在浙江主栽水稻品种和稻瘟菌生理小种鉴别品种中的分布
Figure 2. Distribution of 6 rice resistant genes in CRZ and PRI cultivars
表 1 用于PCR扩增的分子标记
Table 1. Functional markers used for resistant gene amplification
基因
Gene引物名称
Primer name引物序列(5′-3′)
Primer sequence(5′-3′)片段大小
Frangment size/bp抗感性
Resistance and susceptibilityPil M-Pi1 F:GAACAATGCCCAAACTTGAGA
R:GGGTCCACATGTCAGTGAGC460 抗病 Pi9/Piz M-Pi9/Piz F:GCTGTGCTCCAAATGAGGAT
R:GCGATCTCACATCCTTTGCT291/397 抗病/感病 Pi2/Pizt M-Pi2/Pizt F:CAGCGATGGTATGAGCACAA
R:CGTTCCTATACTGCCACATCG450/282 抗病/感病 Pikh FM143 F:CCCAACATTGGTAGTAGTGC
R:TCCTTCATACGCAACAATCT258/401 抗病/感病 Pikm Pik-1 F:TCGAGTTGCTGGAACAAGGG
R:ACTGCGTTCCCAATGCATGA575 抗病 Pita Pita-1 F:AGCAGGTTATAAGCTAGGCC
R:CTACCAACAAGTTCATCAAA1042 抗病 Pita-2 F:AGCAGGTTATAAGCTAGGCTAT
R:CTACCAACAAGTTCATCAAA1042 感病 
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表 2 浙江省水稻栽培品种、稻瘟病菌生理小种鉴别品种和丽江新团黑谷单基因系的抗性频率
Table 2. Resistance frequencies of CRZ, PRI and LTH monogenic lines
编号
No.品种名称
Variety
name抗性频率
Resistance
frequency/%1 Ⅱ优650 48.94 2 Y两优2号 59.57 3 宁81 43.26 4 Y两优1号 75.89 5 Y两优689 65.25 6 春优84 76.60 7 丰两优香1号1 30.50 8 嘉58 67.38 9 EX119 21.28 10 浙粳86 79.43 11 内五优8015 65.96 12 钱优0508 67.38 13 钱优1号 48.23 14 钱优930 53.19 15 绍糯9714 58.87 16 深两优5814 71.63 17 秀水12 58.16 18 秀水321 81.56 19 秀优378 63.83 20 浙粳99 79.43 21 甬优12 74.47 22 甬优15 54.61 23 甬优17 59.57 24 甬优538 73.05 25 甬优8 44.68 26 粤优938 38.30 27 浙优12 81.56 28 浙糯优1号 53.90 29 中浙优1号 52.48 30 中浙优8 73.0 31 浙粳70 74.47 32 甲农糯 7.80 33 秀水519 70.21 34 宁84 64.54 35 秀水134 74.47 36 嘉禾218 27.66 37 宁88 79.43 38 甬优1540 78.01 39 甬优9号 56.03 40 春优927 65.96 41 合江18 26.95 42 四丰43 27.66 43 东农363 50.35 44 关东51 42.55 45 TTP 54.61 46 珍龙13 46.10 47 IRBL1-CL(Pi1) 34.75 48 IRBL9-W(Pi9) 60.99 49 IRBLZ5-CA(Pi2) 58.87 50 IRBLkh-k3(Pikh) 41.13 51 IRBLkm-Ts(Pikm) 43.97 52 IRBLta-K1(Pita) 17.73 53 IRBLz-Fu(Piz) 31.91 54 IRBLzt-T(Pizt) 43.26 注:编号1~40为浙江省栽培品种，41~46为稻瘟病菌生理小种鉴别品种，47~54为丽江新团黑谷单基因系。
Note:No. 1-40 are CRZ cultivars; No. 41-46, PRI cultivars; No. 47-54, LTH monogenic lines.
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表 3 Pi1、Pi9/Piz、Pi2/Pizt、Pikh、Pikm和Pita 6个抗性基因在浙江省栽培品种和稻瘟病菌生理小种鉴别品种中的分布及基因型
Table 3. Distribution and genotypes of Pi1, Pi9/Piz, Pi2/Pizt, Pikh, Pikm and Pita in CRZ and PRI cultivars
品种
Variety基因Gene Pi1 Pi9/Piz Pi2/Pizt Pikh Pikm Pita Ⅱ优650 Ⅱ you 650 SS SS SS SS SS SS Y两优2号 Y liangyou 2 SS SS SS SS SS RS 宁81 Ning 81 SS RR SS RR R_ SS Y两优1号 Y liangyou 1 SS SS SS SS SS RS Y两优689 Y liangyou 689 SS SS SS RS SS RS 春优84 Chunyou 84 SS SS RS RS R_ RS 丰两优香1号 Fengyouxiang 1 SS SS SS RS SS SS 嘉58 Jia 58 SS SS RR SS R_ SS EX119 SS SS SS RR SS SS 浙粳86 Zhegeng 86 SS SS RR SS R_ RR 内五优8015 Neiwuyou 8015 SS SS SS RS SS SS 钱优0508 Qianyou 0508 SS SS SS RS R_ SS 钱优1号 Qianyou 1 SS SS SS RS SS SS 钱优930 Qianyou 930 SS SS SS RR R_ SS 绍糯9714 Shaonuo 9714 SS RR SS RR SS RR 深两优5814 Shenyou 5814 SS SS SS SS SS RS 秀水12 Xiushui 12 SS RR SS SS R_ RR 秀水321 Xiushui 321 SS SS RR SS R_ RR 秀优378 Xiuyou 378 SS SS RR SS R_ SS 浙粳99 Zhegeng 99 SS SS RR SS R_ RR 甬优12 Yongyou 12 SS SS RR SS SS RR 甬优15 Yongyou 15 SS SS RS SS R_ SS 甬优17 Yongyou 17 SS SS RR SS R_ SS 甬优538 Yongyou 538 SS SS RR RS SS RR 甬优8 Yongyou 8 SS SS SS SS SS RR 粤优938 Yueyou 938 SS SS SS SS SS RR 浙优12 Zheyou 12 SS SS RR SS SS RR 浙糯优1号 Zhenuoyou 1 SS RR SS RR SS RR 中浙优1号 Zhongzheyou 1 SS SS SS SS SS SS 中浙优8 Zhongzheyou 8 SS SS SS SS SS SS 浙粳70 Zhegeng 70 SS SS RR RR R_ RR 甲农糯 Jianongnuo SS SS SS SS SS SS 宁84 Ning 84 SS SS RR SS R_ RR 秀水519 Xiushui 519 SS RR SS SS R_ RR 秀水134 Xiushui 134 SS RR SS SS R_ SS 嘉禾218 Jiahe 218 SS RR SS SS SS SS 宁88 Ning 88 SS SS RR RS SS RR 甬优1540 Yongyou 1540 SS SS RR RR SS RS 甬优9号 Yongyou 9 SS RS SS RS SS RS 春优927 Chunyou 927 SS SS RS SS SS RS 合江18 Hejiang 18 SS RR SS SS SS SS 四丰43 Sifeng 43 SS SS SS SS SS SS 东农363 Dongnong 363 SS RR SS RR R_ SS 关东51 Guandong 51 SS SS SS SS R_ SS TTP R_ SS * RR SS RR 珍龙13 Zhenglong 13 SS SS SS SS SS SS 注: RR:纯合抗病基因型; SS:纯合感病基因型; RS:杂合基因型；*未得到扩增。
Note:RR: resistant homozygous genotype. SS: susceptible homozygous genotype. RS: heterozygous genotype. *: no amplification.
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[1] 郑永利, 施德, 姚晓明, 等.晚稻稻瘟病局部突发流行成因与治理对策探讨[J].浙江农业科学, 2015, 56(1):92-93. http://d.old.wanfangdata.com.cn/Periodical/zjnykx201501029ZHENG Y L, SHI D, YAO X M, et al. Discussion on the causes of local outbreak of rice blast in late rice and its control countermeasures[J]. Journal of Zhejiang Agricultural Sciences, 2015, 56(1):92-93.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zjnykx201501029 [2] 谢子正, 许渭根, 李仁忠, 等. 2014年浙江省水稻稻瘟病流行特点及原因分析[J].中国植保导刊, 2015(3):58-60. doi: 10.3969/j.issn.1672-6820.2015.03.014XIE Z Z, XU W G, LI R Z, et al. Epidemiological characteristics and causes of rice blast in Zhejiang Province in 2014[J]. China Plant Protection, 2015(3):58-60.(in Chinese) doi: 10.3969/j.issn.1672-6820.2015.03.014 [3] 向聪, 雷东阳, 任西明, 等.水稻抗稻瘟病遗传育种研究进展[J].作物研究, 2017(5):547-552. http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201705022XIANG C, LEI D Y, REN X M, et al. Research progress in genetic and breeding of rice blast resistance[J].Crop Research, 2017(5):547-552.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201705022 [4] ANDERSEN J R, LUBBERSTED T.Functional markers in plants[J].Trends in Plant Science, 2003, 8(11):554-560. doi: 10.1016/j.tplants.2003.09.010 [5] 谭令辞, 刘雄伦, 杨婷婷, 等.利用Pi9基因分子标记辅助选择培育抗稻瘟病水稻新品系[J].作物研究, 2015, 29(4):348-351. doi: 10.3969/j.issn.1001-5280.2015.04.04TAN L C, LIU X L, YANG T T, et al. Breeding new rice line with Blast Resistant by molecular marker-assisted selection of the Pi9 Gene[J].Crop Research, 2015, 29(4):348-351.(in Chinese) doi: 10.3969/j.issn.1001-5280.2015.04.04 [6] 行璇, 刘雄伦, 陈海龙, 等.分子标记辅助选择Pi9基因改良R288的稻瘟病抗性[J].作物研究, 2016, 30(5):487-491. http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201605002XING X, LIU X L, CHEN H L, et al. Improving blast resistance of rice restorer R288 by molecular marker-assisted selection of Pi9 Gene[J].Crop Research, 2016, 30(5):487-491.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201605002 [7] 梁毅, 杨婷婷, 谭令辞, 等.水稻广谱抗瘟基因Pigm紧密连锁分子标记开发及其育种应用[J].杂交水稻, 2013, 28(4):63-74. http://d.old.wanfangdata.com.cn/Periodical/zjsd201304022LIANG Y, YANG T T, TAN L C, et al. Development of the molecular marker tightly-linked with the broad-spectrum blast resistance gene Pigm and its breeding practice in Rice[J]. Hybrid Rice, 2013, 28(4): 63-74.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zjsd201304022 [8] 杨丰宇, 李永聪, 刘雄伦, 等.分子标记辅助选择改良早籼稻1701的稻瘟病抗性[J].分子植物育种, 2017(6):2212-2217. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201706026YANG F Y, LI YONGC, LIU X L, et al. Improving blast resistance of early indica rice 1701 by molecular marker-assisted selection for[J].Molecular Plant Breeding, 2017(6):2212-2217.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201706026 [9] 范方军, 王芳权, 刘永峰, 等. Pi-b、Pi-ta、Pikm和Pi54对水稻穗颈瘟的抗性评价[J].华北农学报, 2014, 29(3):221-226. http://d.old.wanfangdata.com.cn/Periodical/hbnxb201403040FAN F J, WANG F Q, LIU Y F, et al. Evaluation of resistance to rice panicle blast with resistant genes Pi-b, Pi-ta, Pikm and Pi54[J].Acta Agriculturae Boreali-Sinica, 2014, 29(3):221-226.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hbnxb201403040 [10] 宋兆强, 刘艳, 王宝祥, 等.稻瘟病抗性基因Pi-ta、Pi-b、Pi54和Pi-km的育种利用价值评价[J].江苏农业学报, 2017, 33(5):968-974. doi: 10.3969/j.issn.1000-4440.2017.05.002SONG Z Q, LIU Y, WANG B X, et al.[J]Application value of blast resistance genes Pi-ta, Pi-b, Pi54 and Pi-km in rice breeding[J]. Jiangsu Journal of Agricultural sciences, 2017, 33 (5): 968-974.(in Chinese) doi: 10.3969/j.issn.1000-4440.2017.05.002 [11] International Rice Research Institute. Standard Evalution System for Rice[M]. 4th ed. Manila: IRRI, 1996:17-18. [12] HUA L X, WU J Z, CHEN C X, et al. The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast[J]. Theoretical & Applied Genetics, 2012, 125(5):1047-1055. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=5fab0b7df029d28060cc59d64d6ece46 [13] 刘开强, 伍豪, 颜群, 等.水稻抗稻瘟病基因Pi1的特异性分子标记开发及利用[J].西南农业学报, 2016, 29(6):1241-1244. http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201606001LIU K Q, WU H, YAN Q, et al. Development and application of specific molecular markers of blast resistance gene Pi1 in rice[J]. Southwest China Journal of Agricultural Sciences, 2016, 29 (6): 1241-1244.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201606001 [14] QU S H, LIU G F, ZHOU B, et al. The broad-spectrum blast resistance gene Pi9/Piz encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice[J].Genetics, 2006, 172(3): 1901-1914. doi: 10.1534/genetics.105.044891 [15] 高利军, 高汉亮, 颜群, 等.4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布[J].杂交水稻, 2010, 25(S1):294-298. http://d.old.wanfangdata.com.cn/Conference/7338573GAO L J, GAO H L, YAN Q, et al. Establishment of markers for four blast genes and marker distribution in rice parents[J].Hybrid Rice, 2010, 25 (S1): 294-298.(in Chinese) http://d.old.wanfangdata.com.cn/Conference/7338573 [16] ZHOU B, Qu S H, LIU G F, et al.The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea[J]. Molecular Plant-Microbe Interactions, 2006, 19(11): 1216-1228. doi: 10.1094/MPMI-19-1216 [17] SHARMA T R, MADHAY M S, SINGH B K, et al. High-resolution mapping, cloning and molecular characterization of the Pi-k (h) gene of rice, which confers resistance to Magnaporthe grisea[J]. Molecular genetics and genomics: MGG, 2005, 274(6): 569-578. doi: 10.1007/s00438-005-0035-2 [18] 王军, 杨杰, 朱金燕, 等.稻瘟病抗病基因Pi-kh功能标记的开发及江苏粳稻品种中Pi-kh的变异[J].中国水稻科学, 2014, 28(2):141-14. doi: 10.3969/j.issn.1001-7216.2014.02.005WANG J, YANG J, ZHU J Y, et al. Development of a functional marker for rice blast resistance gene Pi-kh and natural variation atPi-kh in Japonica rice in Jiangsu province[J].Chinese Journal of Rice Science, 2014, 28 (2): 141-147.(in Chinese) doi: 10.3969/j.issn.1001-7216.2014.02.005 [19] ASHIKAWA I, HAYASHI N, YAMANE H, et al. Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance[J]. Genetics, 2008, 180(4): 2267-2276. doi: 10.1534/genetics.108.095034 [20] 徐小金.稻瘟病抗性基因资源的挖掘及其育种利用[D].金华: 浙江师范大学, 2016.XU X J. Exploitation and breeding application of rice blast resistance gene resources[D]. Jinhua: Zhejiang Normal University, 2016.(in Chinese) [21] BRYAN G T, WU K S, FARRALL L, et al. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta[J]. The Plant Cell Online, 2000, 12(11): 2033-2045. doi: 10.1105/tpc.12.11.2033 [22] JIA Y L, WANG Z H, SINGH P. Development of Dominant Rice Blast, Pi-ta, Resistance Gene Markers[J]. Crop Science, 2002, 42(6):2145-2149. doi: 10.2135/cropsci2002.2145 [23] JIANG H C, FENG Y T, BAO L, et al. Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding[J]. Molecular Breeding, 2012, 30(4):1679-1688. doi: 10.1007/s11032-012-9751-6 [24] 陈红旗, 陈宗祥, 倪深, 等.利用分子标记技术聚合3个稻瘟病基因改良金23B的稻瘟病抗性[J].中国水稻科学, 2008, 22(1):23-27. doi: 10.3321/j.issn:1001-7216.2008.01.004CHEN H Q, CHEN Z X, NI S, et al. Pyramiding three genes with resistance to blast by marker-assisted to improve rice blast resistance of Jin23B[J]. Chinese Journal of Rice Science, 2008, 22(1): 23-27.(in Chinese) doi: 10.3321/j.issn:1001-7216.2008.01.004 [25] 柳武革, 王丰, 金素娟, 等.利用分子标记辅助选择聚合Pi-1和Pi-2基因改良两系不育系稻瘟病抗性[J].作物学报, 2008, 34(7):1128-1136. http://d.old.wanfangdata.com.cn/Periodical/zuowxb200807004LIU W G, WANG F, JIN S J, et al. Improvement of rice blast resistance of TGMS Line by pyramising of Pi-1 and Pi-2 through molecular marker-assisted selection[J].Acta Agromomica Sinica, 2008, 34(7): 1128-1136.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowxb200807004 [26] DIVYA B, ROBIN S, RABINDRAN R, et al. Marker assisted backcross breeding approach to improve blast resistance in Indian rice (Oryza sativa) variety ADT43[J]. Euphytica, 2014, 200(1):79. doi: 10.1007/s10681-014-1182-5 [27] 于苗苗, 戴正元, 潘存红, 等.广谱稻瘟病抗性基因Pigm和Pi2的抗谱差异及与Pi1的互作效应[J].作物学报, 2013, 39(11):1927-1934. http://d.old.wanfangdata.com.cn/Periodical/zuowxb201311002YU M M, DAI Z Y, PAN C H, et al. Resistance spectrum difference between two broad-spectrum blast resistance genes, Pigmand Pi2, and their interaction effect on Pi1[J].Acta Agromomica Sinica, 2013, 39 (11): 1927-1934.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowxb201311002 [28] 邢鹏, 张幸, 李冬梅, 等.稻瘟病抗性基因在主要育种亲本中的分布研究[J].分子植物育种, 2015, 13(3):505-512. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201503004XING P, ZHANG X, LI D M, et al. Research on the distribution of blast resistance genes in main rice breeding parents[J].Molecular Plant Breeding, 2015, 13(3): 505-512.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201503004 [29] 孙国昌, 杜新法, 陶荣祥, 等.水稻稻瘟病防治策略和21世纪研究展望[J].植物病理学报, 1998(4):289-292. doi: 10.3321/j.issn:0412-0914.1998.04.001SUN G C, DU X F, TAO R X, et al. Control tactics and prospect of rice blast research in 21th century[J]. Acta Phytopathologica Sinica, 1998 (4): 289-292.(in Chinese) doi: 10.3321/j.issn:0412-0914.1998.04.001 [30] 柴荣耀, 杜新法, 毛雪琴, 等.水稻不同类型品种上的稻瘟病菌群体小种的演变[J].浙江农业学报, 1999, 11(6):297-300. doi: 10.3969/j.issn.1004-1524.1999.06.005CHAI H, DU X F, MAO X Q, et al. Race evolution of Magnaporthe grisea on different rice varieties[J].Acta Agriculturae Zhejianggensis, 1999, 11(6): 297-300.(in Chinese) doi: 10.3969/j.issn.1004-1524.1999.06.005 [31] 任鄄胜, 肖陪村, 陈勇, 等.水稻稻瘟病病菌研究进展[J].现代农业科学, 2008(1):19-23. http://d.old.wanfangdata.com.cn/Periodical/ynnydxxb200101019REN J S, XIAO Y C, CHEN Y, et al. The research progress on rice blast fungus[J]. Modern Agricultural Science, 2008 (1): 19-23.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/ynnydxxb200101019 -
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