寄主对斜纹夜蛾实验种群生殖力的影响

蒋立奔; 林硕; 陈勇; 余芸; 陈艺欣; 田厚军; 魏辉

doi:10.19303/j.issn.1008-0384.2019.02.015

寄主对斜纹夜蛾实验种群生殖力的影响

doi: 10.19303/j.issn.1008-0384.2019.02.015

蒋立奔^{1, 2,},
林硕^1,,
陈勇¹,
余芸¹,
陈艺欣¹,
田厚军¹,
魏辉^1, ,

1.
福建省农业科学院植物保护研究所/福建省作物有害生物监测与治理重点实验室/农业部福州作物有害生物科学观测试验站, 福建福州 350003
2.
江苏丘陵地区南京农业科学研究所, 江苏南京 210046

基金项目:

福建省自然科学基金 2015J05061

福建省科技计划项目——省属公益类科研院所基本科研专项 2016R1023-5

福建省科技重大专项 2017NZ0003-1-4

福建省财政专项——福建省农业科学院科技创新团队建设项目 STIT2017-1-8

福建省农业科学院科研引导性项目 AC2017-6

国家重点研发计划课题 2018YFD0201209

详细信息

作者简介:
蒋立奔(1985-), 男, 博士, 助理研究员, 研究方向:昆虫化学生态学(E-mail:jiangliben@163.com)

林硕(1982-), 男, 硕士, 研究实习员, 研究方向:昆虫化学生态学(E-mail:13923757@qq.com)

通讯作者:
魏辉(1972-), 男, 博士, 研究员, 研究方向:昆虫化学生态学(E-mail:weihui@faas.cn)

中图分类号: S435
计量
- 文章访问数: 1359
- HTML全文浏览量: 181
- PDF下载量: 32
- 被引次数: 0
出版历程
- 收稿日期: 2019-01-03
- 修回日期: 2019-02-01
- 刊出日期: 2019-02-28

Fecundity of Adult Spodoptera litura Affected by Diet

JIANG Li-ben^{1, 2
,},
LIN Shuo^1
,,
CHEN Yong¹,
YU Yun¹,
CHEN Yi-xin¹,
TIAN Hou-jun¹,
WEI Hui^{1
, ,}

1.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Fuzhou Scientific Observing and Experimental Station of Crop Pests of Ministry of Agriculture, Fuzhou, Fujian 350003, China
2.
Nanjing Agricultural Institute of Jiangsu Hilly Region, Nanjing, Jiangsu 210046, China

摘要

摘要: 目的斜纹夜蛾Spodoptera litura Fabricius是一种多食性害虫，繁殖能力强，全球范围内均有分布。但是寄主对斜纹夜蛾繁殖力影响机制还不清楚。开展寄主对斜纹夜蛾实验种群生殖力的影响研究，为斜纹夜蛾的预测预报以及防治提供理论依据。方法通过单头饲养的方法，研究斜纹夜蛾取食甘蓝、空心莲子草和地瓜后的产卵量、卵黄原蛋白（Vg）及卵黄原蛋白受体（VgR）表达的变化。结果幼虫取食不同寄主的成虫寿命和产卵前期没有显著性差异，但成虫的产卵量、Vg及VgR表达量有显著差异。取食人工饲料的斜纹夜蛾产卵量最多，为（889±43.0）粒，其次为取食地瓜（496±53.1）粒、空心莲子草（362±37.3）粒，和甘蓝（193±9.7）粒。取食甘蓝、空心莲子草和地瓜的斜纹夜蛾雌虫卵巢Vg和VgR的相对表达量仅为取食人工饲料斜纹夜蛾的40%~80%，且取食不同寄主的斜纹夜蛾Vg和VgR的相对表达量与产卵量呈正相关关系。结论斜纹夜蛾取食不同寄主后，其卵巢内的Vg和VgR表达量存在差异，从而影响到斜纹夜蛾卵子发育，最终造成其生殖力存在差异。
- 斜纹夜蛾 /
- 产卵量 /
- 卵黄原蛋白 /
- 卵黄原蛋白受体
Abstract: Objective Effect of forage on fecundity of the highly reproductive and widely spread polyphagous pest, Spodoptera litura Fabricius, was studied. Method Under the laboratory conditions, insects were raised individually on different diet or host plant to monitor the effects on the fecundity, vitellogenin (Vg) and vitellogenin receptor (VgR) of adult S. litura. Result No significant differences were observed on the lifespan and response of the pre-oviposition insects as they were fed on a formulated diet, Brassica oleracea, Alternanthera philoxeroides or Ipomoea batatas. On the other hand, the fecundity, Vg and VgR of the adult females differed significantly whether they were on the formulated diet (control) or the host materials. An individual adult female on the formulated diet would produce 889±43.0 eggs; whereas, one on I. batatas laid 496±53.1 eggs; one on A. philoxeroides, 362±37.3 eggs; and, one on B. oleracea, 193±9.7 eggs. The relative Vg and VgR transcript levels of the female moth population fed on the host materials were merely 40% to 80% of those of control. And, those measurements correlated well with the fecundity of the insect. Conclusion After feeding on various hosts, the titers of Vg and VgR of S. litura differed in comparison to control diet which directly affected the egg development and fertility of the insect.
- Spodopteralitura /
- fecundity /
- vitellogenin /
- vitellogenin receptor

HTML全文

图 1 不同寄主对斜纹夜蛾成虫寿命及产卵的影响

Figure 1. Effects of various hosts on lifespan and fecundity of adult S. litura

下载: 全尺寸图片幻灯片

图 2 不同食物对雌蛾日产卵量的影响

Figure 2. Effects of various hosts on daily oviposition of female moths

下载: 全尺寸图片幻灯片

图 3 寄主对斜纹夜蛾雌虫卵黄原蛋白(Vg)和卵黄原蛋白受体(VgR)表达量动态变化

Figure 3. RT-qPCR analysis of Vg and VgR expressed in adult female moths

下载: 全尺寸图片幻灯片

表 1 qPCR中所用引物序列

Table 1. Sequences of primers used for qPCR

基因名称 Primer name	基因序列号 GenBank accession no.	引物序列 Sequence
SlVg	EU095334	5′-CACTCCGCTGTATCTCGCCTTCT-3′
		5′-CGGGGGCATTGTCTTGTTCCAC-3′
SlVgR	LOC111350990	5′-TGGCACGGATGAGCACGGTT-3′
		5′-GGCGTCTCATCGGATCCGTCAT-3′
β-Actin	DQ494753	5′-GGCCAACAGGGAGAAGATGAC-3′
		5′-CCGGAGTCCAGGACGATAC-3′

下载: 导出CSV

参考文献(24)

[1]	AQUEEL M A, LEATHER S R. Effect of nitrogen fertilizer on the growth and survival of Rhopalosiphum padi (L.) and Sitobion avenae (F.)(Homoptera:Aphididae) on different wheat cultivars[J]. Crop Protection, 2011, 30(2):216-221. doi: 10.1016/j.cropro.2010.09.013
[2]	钦俊德, 王琛柱.论昆虫与植物的相互作用和进化的关系[J].昆虫学报, 2001, 44(3):360-365. doi: 10.3321/j.issn:0454-6296.2001.03.019 QIN J D, WANG C Z. The relation of interaction between insects and plants to evolution[J]. Acta Entomologica Sinica, 2001, 44(3):360-365.(in Chinese) doi: 10.3321/j.issn:0454-6296.2001.03.019
[3]	姚晓明.不同寄主植物对斜纹夜蛾生殖力的影响及其机制研究[D].扬州: 扬州大学, 2009. http://d.wanfangdata.com.cn/Thesis/Y1501169 YAO X M. The mechanism of different host plants on the fecundity of Spodoptera litura (Lepidoptera: Noctuidae)[D]. Yangzhou: Yangzhou University, 2009.(in Chinese) http://d.wanfangdata.com.cn/Thesis/Y1501169
[4]	SNIGIREVSKAYA E S, RAIKHEL A S. Receptor-mediated endocytosis of yolk proteins in insect oocytes[C]//Raikhel A S. Progress in vitellogenesis: reproductive biology of invertebrates. CRC Press, 2005, 12(B): 215-244.
[5]	GIORGI F, SNIGIREVSKAYA E S, RAIKHEL A S. The cell biology of yolk protein precursor synthesis and secretion[C]//Raikhel A S. Progress in vitellogenesis: reproductive biology of invertebrates. CRC Press, 2005, 12(B): 33-68.
[6]	TUFAIL M, TAKEDA M. Molecular characteristics of insect vitellogenins[J]. Journal of Insect Physiology, 2008, 54(12):1447-1458. doi: 10.1016/j.jinsphys.2008.08.007
[7]	TUFAIL M, NAGABA Y, ELGENDY A M, et al. Regulation of vitellogenin genes in insects[J]. Entomological Science, 2014, 17(3):269-282. doi: 10.1111/ens.2014.17.issue-3
[8]	石学娟.斜纹夜蛾寄主适生性与性信息素诱测等监控技术的作用评估[D].扬州: 扬州大学, 2008. http://cdmd.cnki.com.cn/Article/CDMD-11117-2008087258.htm SHI X J. Evaluation of the monitoring techniques of host fitness and sex pheromone induction in Spodoptera litura (Lepidoptera: Noctuidae)[D]. Yangzhou: Yangzhou University, 2008.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-11117-2008087258.htm
[9]	RAO G R, WIGHTMAN J A, RAO D R. World review of the natural enemies and diseases of Spodoptera litura (F.)(Lepidoptera:Noctuidae)[J]. International Journal of Tropical Insect Science, 1993, 14(3):273-284. doi: 10.1017/S1742758400014764
[10]	秦厚国, 汪笃栋, 丁建, 等.斜纹夜蛾寄主植物名录[J].江西农业学报, 2006, 18(5):51-58. doi: 10.3969/j.issn.1001-8581.2006.05.017 QIN H G, WANG D D, DING J, et al. Host plant of Spodoptera litura[J]. Acta Agriculturae Jiangxi, 2006, 18(5):51-58.(in Chinese) doi: 10.3969/j.issn.1001-8581.2006.05.017
[11]	KUMAR K, CHAPMAN R B. Sublethal effects of insecticides on the diamondback moth Plutella xylostella (L.)[J]. Pesticide Science, 1984, 15(4):344-352. doi: 10.1002/(ISSN)1096-9063
[12]	AGRAWAL A A. Mechanisms, ecological consequences and agricultural implications of tri-trophic interactions[J]. Current Opinion in Plant Biology, 2000, 3(4):329-335. doi: 10.1016/S1369-5266(00)00089-3
[13]	刘佳, 周勇, 朱航, 等.斜纹夜蛾抗药性监测及茚虫威对其解毒代谢酶的影响[J].昆虫学报, 2016, 59(11):1254-1262. http://d.old.wanfangdata.com.cn/Periodical/kcxb201611014 LIU J, ZHOU Y, ZHU H, et al. Monitoring of insecticide resistance in Spodoptera litura (Lepidoptera:Noctuidae) and the effect of indoxacarb on its detoxification enzymes[J]. Acta Entomologica Sinica, 2016, 59(11):1254-1262.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/kcxb201611014
[14]	SANDHYARANI K, RANI P U. Morphometric and developmental consequences in Spodoptera litura due to feeding on three varieties of sweet potato plants (Ipomoea batata)[J]. Phytoparasitica, 2013, 41(3):317-325. doi: 10.1007/s12600-013-0292-2
[15]	祝树德, 陆自强, 陈丽芳, 等.温度和食料对斜纹夜蛾种群的影响[J].应用生态学报, 2000, 11(1):111-114. doi: 10.3321/j.issn:1001-9332.2000.01.028 ZHU S D, LU Z Q, CHEN L F, et al. Effect of temperature and food on Spodoptera litura population[J]. Chinese Journal of Applied Ecology, 2000, 11(1):111-114.(in Chinese) doi: 10.3321/j.issn:1001-9332.2000.01.028
[16]	涂业苟, 吴孔明, 薛芳森, 等.不同寄主植物对斜纹夜蛾生长发育、繁殖及飞行的影响[J].棉花学报, 2008, 20(2):105-109. doi: 10.3969/j.issn.1002-7807.2008.02.005 TU Y G, WU K M, XUE F S, et al. 1nfluence of host plants on larval development, adult fecundity and flight ability of the common cutworm, Spodoptera litura[J]. Cotton Science, 2008, 20(2):105-109.(in Chinese) doi: 10.3969/j.issn.1002-7807.2008.02.005
[17]	CHEN Y, LU C, LI M, et al. Adverse effects of rice gall dwarf virus upon its insect vector Recilia dorsalis (Hemiptera:Cicadellidae)[J]. Plant Disease, 2016, 100(4):784-790. doi: 10.1094/PDIS-06-15-0713-RE
[18]	姚苗苗, 杨茂发, 武承旭, 等.不同寄主植物对斜纹夜蛾生长发育的影响[J].广东农业科学, 2016, 43(1):93-97. doi: 10.3969/j.issn.1004-874X.2016.01.018 YAO M M, YANG M F, WU C X, et al. Effects of different host plants on growth and development of Spodoptera litura[J]. Guangdong Agricultural Sciences, 2016, 43(1):93-97.(in Chinese) doi: 10.3969/j.issn.1004-874X.2016.01.018
[19]	辛未一.两种寄主植物对斜纹夜蛾繁殖及卵黄原蛋白基因(Vg)表达的影响[D].扬州: 扬州大学, 2016. http://cdmd.cnki.com.cn/Article/CDMD-11117-1016284225.htm XIN W Y. Effects of two host plants on fecundity and vitellogenin gene expression of Spodoptera litura Fabricius[D]. Yangzhou: Yangzhou University, 2016.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-11117-1016284225.htm
[20]	栾玉柱, 顾继伟, 李美玲.不同寄主植物对斜纹夜蛾的影响及机制探讨[J].江苏农业科学, 2013, 41(11):142-144. doi: 10.3969/j.issn.1002-1302.2013.11.053 LUAN Y Z, GU J W, LI M L. Effects of different host plants on Spodoptera litura[J]. Jiangsu Agricultural Sciences, 2013, 41(11):142-144.(in Chinese) doi: 10.3969/j.issn.1002-1302.2013.11.053
[21]	邓海滨, 王晓容, 陈永明.不同寄主作物对斜纹夜蛾分布和生长发育的影响研究[J].广东农业科学, 2006(4):53-55. doi: 10.3969/j.issn.1004-874X.2006.04.018 DENG H B, WANG X R, CHEN Y M.Effects of different host plants on distribution and development of Spodoptera litura[J]. Guangdong Agricultural Sciences, 2006(4):53-55.(in Chinese) doi: 10.3969/j.issn.1004-874X.2006.04.018
[22]	GHUMARE S S, MUKHERJEE S N. Performance of spodoptera litura fabricius on different host plants:influence of nitrogen and total phenolics of plants and mid-gut esterase activity of the insect[J]. Indian Journal of Experimental Biology, 2003, 41(8):895-899. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_JJ029768313
[23]	WHEELER D E, BUCK N A. A role for storage proteins in autogenous reproduction in aedes atropalpus[J]. Journal of Insect Physiology, 1996, 42(10):961-966. doi: 10.1016/0022-1910(96)00049-2
[24]	孙建新, 谭璟宪.医学昆虫卵黄原蛋白生物合成的激素调节[J].国外医学(寄生虫病分册), 1990(2):63-66. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK000000733946 SUN J X, TAN J X. Hormonal regulation of vitellogenin biosynthesis in insects[J]. Foreign Medicine (Parasite Section), 1990(2):63-66.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK000000733946