不同油菜品种对陕西省根肿菌的抗性鉴定与评价

张振兰; 李永红; 李建厂; 赵志国

doi:10.19303/j.issn.1008-0384.2019.05.011

不同油菜品种对陕西省根肿菌的抗性鉴定与评价

doi: 10.19303/j.issn.1008-0384.2019.05.011

1.
陕西省杂交油菜研究中心, 陕西杨凌 712100
2.
太白县农业技术推广服务中心, 陕西太白 721600

基金项目:

国家重点研发计划项目 2018YFD0200904-11

陕西省重点研发计划项目 2018ZDXM-NY-001

详细信息

作者简介:
张振兰(1972-), 女, 助理研究员, 主要从事植物保护工作(E-mail:zhenlancool@163.com)

通讯作者:
李永红(1966-), 男, 研究员, 主要从事植物保护工作(E-mail:yhlion@126.com)

中图分类号: S435
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- 被引次数: 0
出版历程
- 收稿日期: 2019-03-14
- 修回日期: 2019-04-25
- 刊出日期: 2019-05-28

Disease-Resistance of Shaanxi Brassica napus to Various Plasmodiophora brassicae

1.
Hybrid Rapeseed Research Center of Shaanxi Province, Yangling, Shaanxi 712100, China
2.
Taibai Agricultural Technology Promotion Service Center, Taibai, Shaanxi 712600, China

摘要

摘要: 目的为明确陕西省不同地区油菜根肿菌的致病性，筛选抗根肿病的油菜种质资源。方法采用根部注射的室内接种发病方法，对陕西省4个不同地区油菜根肿菌的致病性和15份甘蓝型油菜品种的根肿病抗性进行鉴定。结果来源于陕西省西乡县、南郑县、勉县和太白县的油菜根肿菌的致病性存在显著差异，其中勉县根肿菌的致病性最强，南郑县根肿菌的致病性最弱。参试的15个油菜品种的抗性存在显著差异，引进的5个抗性资源华杂62R、华双5R、1628A×根抗、171R和172R的根肿病抗性显著高于陕西省的10个栽培品种。聚类分析结果显示引进的5个抗性资源聚为抗病一类，陕西省的10个栽培品种聚在感病一类。结论陕西省4个地区根肿菌存在生理小种差异。引进的5个抗根肿病资源对这4个地区根肿菌均表现为较强的抗性，可以作为当地根肿病病区主栽品种及育种的抗性资源。
- 甘蓝型油菜 /
- 根肿病 /
- 芸薹属根肿菌
Abstract: Objective Pathogenicity of various strains of Plasmodiophora brassicae on Brassica napus cultivars was investigated. Method Four different P. brassicaes strains were collected from the counties of Xixiang, Nanzheng, Mian, and Taibai in Shaanxi, and the disease-resistance of 15 varieties of B. napus toward them by inoculating in the roots determined in an indoor test. Result The pathogenicity of the various strains were significantly different.The P. brassicaes collected from Mian county was the strongest among all, while from Nanzheng county the weakest. The disease-resistance of the 15 rapeseed varieties differed significantly as well. The 5 significantly more resistant varieties, 62R, Huashuang 5R, 1628A×genkang, 171R, and 172R, were not native Shaanxi cultivars. The cluster analysis classified them as disease-resistant toward the clubroot disease and the 10 local germplasms disease-sensitive. Conclusion Physiological differences existed among the strains of P. brassicaes from the 4 regions in Shaanxi. The 5 identified clubroot disease-resistant rapeseed cultivars would be promising candidates for breeding new varieties for cultivation in the province.
- Brassica napus /
- clubroot disease /
- Plasmodiophora brassicae

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图 1 15个油菜品种对4个地区根肿病菌的抗性系统聚类

注：A为发病率，B为病情指数。

Figure 1. Cluster dendrogram on disease-resistance of 15 rapeseed cultivars to P. brassicae collected from 4 counties in Shaanxi

Note:A.Incidence, B.Disease index.

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表 1 15个油菜品种对4个地区根肿菌的抗性结果

Table 1. Disease-resistance of 15 rapeseed varieties to P. brassicae collected from 4 counties in Shaanxi

品种 Variety	太白县Taibai county			西乡县Xixiang county			南郑县Nanzheng county			勉县Mian county
品种 Variety	A/%	B	C	A/%	B	C	A/%	B	C	A/%	B	C
华杂62R Huaza62R	61.1	36.4	R	38.9	19.1	HR	36.1	13.3	HR	55.6	34.6	R
华双5R Huashuang5R	5.6	1.9	HR	2.8	2.2	HR	0.0	0.0	HR	38.9	14.8	HR
1628A×根抗1628A×genkang	8.3	2.8	HR	8.3	4.6	HR	0.0	0.0	HR	47.2	20.7	HR
171R	19.4	4.6	HR	0.0	0.0	HR	0.0	0.0	HR	33.3	16.0	HR
172R	5.6	1.2	HR	11.1	1.9	HR	0.0	0.0	HR	33.3	20.4	HR
渝油21 Yuyou21	86.1	57.7	S	91.7	52.8	S	63.9	31.8	R	86.1	60.8	S
赣油杂6号Ganyouza No.6	88.9	56.8	S	80.6	48.5	LR/LS	61.1	36.4	R	100.0	72.8	S
绵新油38 Mianxinyou38	86.1	58.3	S	91.7	57.7	S	69.4	37.3	R	100.0	74.1	S
蓉油18 Rongyou18	80.6	47.8	LR/LS	94.4	63.0	S	86.1	47.2	LR/LS	100.0	73.5	S
福油518 Fuyou518	66.7	41.4	LR/LS	83.3	54.3	S	72.2	30.9	R	91.7	67.0	S
沣油737 Fengyou737	100.0	74.7	S	97.2	74.4	S	88.3	48.8	LR/LS	100.0	76.5	S
秦优7号Qinyou No.7	100.0	82.1	HS	97.2	76.2	S	88.6	63.8	S	97.2	75.0	S
陕油28 Shanyou28	100.0	70.4	S	91.7	69.4	S	97.2	68.8	S	100.0	77.8	S
秦优10号Qinyou No.10	97.2	72.5	S	94.7	67.4	S	83.3	40.7	LR/LS	100.0	79.0	S
油研52 Youyan52	97.2	75.6	S	91.7	75.0	S	94.4	63.6	S	100.0	78.4	S
注：A为发病率，B为病情指数，C为抗性。 Note：A.Incidence，B.Disease index，C.Resistant.

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表 2 不同地区菌种间致病性差异显著性分析

Table 2. Pathogenicity of P. brassicae collected from 4 counties in Shaanxi

地区 Region	发病率Incidence			病情指数Disease index
地区 Region	均值 Mean	5%显著水平 Significant in 5%	1%极显著水平 Significant in 1%	均值 Mean	5%显著水平 Significant in 5%	1%极显著水平 Significant in 1%
勉县Mian county	78.9	a	A	56.1	a	A
太白县Taibai county	66.9	b	B	45.6	b	B
西乡县Xixiang county	65.0	b	B	44.4	b	B
南郑县Nanzheng county	56.1	c	C	32.2	c	C

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表 3 不同油菜品种根肿病抗性差异显著性分析

Table 3. Disease-resistance of 15 rapeseed cultivars

地区 Region	发病率Incidence			病情指数Disease index
地区 Region	均值 Mean	5%显著水平 Significant in 5%	1%极显著水平 Significant in 1%	均值 Mean	5%显著水平 Significant in 5%	1%极显著水平 Significant in 1%
陕油28 Shanyou28	97.2	a	A	71.6	ab	AB
沣油737 Fengyou737	96.4	ab	A	68.6	ab	AB
油研52 Youyan52	95.9	ab	A	73.2	a	AB
秦优7号Qinyou No.7	95.8	ab	A	74.3	a	A
秦优10号Qinyou No.10	93.8	ab	AB	64.9	b	BC
蓉油18 Rongyou18	90.3	abc	ABC	57.9	c	CD
绵新油38 Mianxinyou38	86.8	bcd	ABC	56.9	cd	CDE
赣油杂6号Ganyouza No.6	82.6	cd	BC	53.6	cde	DE
渝油21 Yuyou21	81.9	cd	BC	50.8	de	DE
福油518 Fuyou518	78.5	d	C	48.4	e	E
华杂62R Huaza62R	47.9	e	D	25.9	f	F
1628Rx根抗1628A×genkang	16.0	f	E	7.0	g	G
171R	13.2	f	E	5.2	g	G
172R	12.5	f	E	5.9	g	G
华双5R Huashuang5R	11.8	f	E	4.7	g	G

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