基于主成分分析的刺葡萄实生单株苗期性状综合评价

许红叶; 李婷; 臧运祥; 徐凯; 高永彬; 郑伟尉

doi:10.19303/j.issn.1008-0384.2019.07.003

基于主成分分析的刺葡萄实生单株苗期性状综合评价

doi: 10.19303/j.issn.1008-0384.2019.07.003

浙江农林大学农业与食品科学学院, 浙江杭州 311300

基金项目:

浙江省科技厅项目——十三五果品新品种选育重大专项 2016C02052-6

浙江省基础公益研究计划项目 LGN19C150008

详细信息

作者简介:
许红叶(1995-), 女, 硕士, 研究方向:园艺植物栽培生理(E-mail:1712832437@qq.com)

通讯作者:
郑伟尉(1980-), 女, 博士, 副教授, 研究方向:园艺植物资源利用与创新(E-mail:zhengww@zafu.edu.cn)

中图分类号: S663.1
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- 被引次数: 0
出版历程
- 收稿日期: 2019-02-12
- 修回日期: 2019-05-14
- 刊出日期: 2019-07-20

Characterization of Seed-derived Vitis davidii Seedings by Principal Component Analysis

College of Agriculture and Food Science, Zhejiang A & F University, Hangzhou, Zhejiang 311300, China

摘要

摘要: 目的建立一套适合刺葡萄实生单株苗期性状的评价方法。方法测定了刺葡萄实生单株干径、新梢长度、叶片叶绿素含量、叶面积、叶片干鲜重、叶片叶形结构参数等16个主要性状，并应用主成分分析法对其进行综合评价。结果刺葡萄实生单株主要生长性状均出现较大变异，尤其是新梢长度、叶片叶绿素含量、干径等变异较大。选取方差累积贡献率为86.254%的前6个主成分来评价刺葡萄实生单株，决定第1主成分的是干径、新梢长度、叶绿素含量、叶面积、叶片鲜重、叶片干重；决定第2主成分的是中脉长、上侧脉长、下侧脉长、叶柄脉长；决定第3主成分的是叶宽和上中侧脉夹角。结论以6个主成分及单个主成分所对应的特征值占所提取主成分总的特征值之和的比例作为权重，构建刺葡萄实生单株苗期性状的综合评价模型F_综=0.38F₁+0.24F₂+0.12F₃+0.10F₄+0.09F₅+0.06F₆，综合得分排名前两位的单株分别为S4和S35。
- 刺葡萄 /
- 主成分分析法 /
- 苗期 /
- 综合评价
Abstract: Objective To establish a method for evaluating the characteristics of seed-derived Vitis davidii seedlings. Method Sixteen characteristics including stem girth, shoot length, leaf chlorophyll content, leaf area, leaf dry and fresh weight, and blade structure of the seedlings were determined for a comprehensive evaluation by principal component analysis. Result Significant variations were found on the growth of the seedlings, especially on shoot length, leaf chlorophyll content, and stem girth. The first 6 principal components made up 86.254% of the total contribution. The first principal component was determined by the stem girth, shoot length, chlorophyll content, leaf area, and fresh and dry weights of the leaves; the second principal component by the lengths of midrib, upper lateral vein, lower lateral vein, and downmost vein; and, the third principal component by the leaf width and the angle between upper lateral vein and midrib. Conclusion Using the eigenvalues corresponding to the 6 principal components and the ratio of single to extracted principal component, a comprehensive evaluation model for the seed-derived V.davidii seedlings was established. Accordingly, S4 and S35 scored the highest among the specimens. A rapid method to screen V.davidii resources at seedling stage for cultivar selection and new variety breeding had thus become available.
- Vitis davidii /
- principal component analysis /
- seedling stage /
- comprehensive evaluation

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图 1 叶片叶形结构参数

Figure 1. Schematic components for describing blade structure of seedlings

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图 2 刺葡萄实生单株苗期干径比较

Figure 2. Stem girth of V. davidii seedlings

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图 3 刺葡萄实生单株苗期新梢长度比较

Figure 3. Shoot length of V. davidii seedlings

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图 4 刺葡萄实生单株苗期叶片叶绿素含量比较

Figure 4. Leaf chlorophyll content of V. davidii seedlings

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图 5 刺葡萄实生单株苗期叶片鲜重、干重比较

Figure 5. Dry and fresh weigh of V. davidii seedling leaves

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图 6 刺葡萄实生单株苗期叶面积比较

Figure 6. Leaf area of V. davidii seedlings

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图 7 刺葡萄实生单株苗期叶脉长比较

Figure 7. Vein length of V. davidii seedlings

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图 8 刺葡萄苗期叶宽比较

Figure 8. Leaf width of V. davidii seedlings

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图 9 刺葡萄实生单株苗期上裂刻基部距叶脉基点长、下裂刻基部距叶脉基点长比较

Figure 9. Distance between upper fissure or inferior fissure base and vein base on leaves of V. davidii seedlings

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图 10 刺葡萄苗期叶脉夹角比较

Figure 10. Angle between veins on leaves of V. davidii seedlings

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表 1 刺葡萄苗期性状的特征值和累计方差贡献率

Table 1. Eigenvalues and accumulative contribution of various characteristics of V. davidii seedlings

主成分 The principal components	特征值 The eigenvalue	方差贡献率 Variance contribution rate/%	累计方差贡献率 Cumulative variance contribution rate/%
1	5.177	32.359	32.359
2	3.265	20.409	52.768
3	1.614	10.085	62.853
4	1.430	8.937	71.790
5	1.308	8.176	79.966
6	1.006	6.288	86.254

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表 2 主成分在各苗期性状指标上的因子荷载矩阵

Table 2. Rotated component matrix of principal components

性状 Characters	主成分The principal components
性状 Characters	1	2	3	4	5	6
干径Stem diameter	0.930	0.057	0.184	0.110	-0.028	0.253
新梢长度New shoots length	0.890	-0.219	0.272	0.223	0.505	-0.114
叶绿素含量Chlorophyll content	0.888	0.189	0.409	-0.091	-0.624	0.191
叶片鲜重Leaf fresh weight	0.864	-0.096	-0.133	-0.008	-0.105	0.143
叶片干重Leaf dry weight	0.663	0.064	-0.233	-0.015	0.385	0.268
叶面积Leaf area	0.568	-0.074	0.275	-0.089	-0.283	-0.619
中脉长Midrib length	0.089	0.905	0.150	-0.089	-0.090	-0.080
上侧脉长Upper lateral vein length	0.077	0.860	0.060	-0.125	0.071	0.026
下侧脉长Lower lateral vein length	0.338	0.800	0.001	-0.145	0.146	0.087
叶柄脉长Downmost vein length	0.285	0.559	-0.100	-0.175	0.087	0.013
叶宽Leaf width	0.249	-0.185	0.661	0.132	-0.102	-0.147
上裂刻基部距叶脉基点长Distance from upper fissure base to point of leaf vein	0.265	-0.215	-0.299	0.758	-0.094	-0.060
下裂刻基部距叶脉基点长Distance from inferior fissure to base point of leaf vein	0.319	-0.161	-0.343	0.646	-0.100	-0.054
上中侧脉夹角Angle between upper lateral and midrib veins	0.092	-0.106	0.690	-0.099	0.100	-0.225
上下侧脉夹角Angle between upper and lower later	0.061	0.540	-0.163	-0.142	0.284	0.554
下侧脉与叶柄脉夹角Angle between lower lateral veins and downmost veins	-0.257	0.488	-0.080	-0.486	0.511	0.130

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表 3 刺葡萄苗期性状评价结果

Table 3. Evaluation on characteristics of V. davidiii seedings

编号 Serial number	F₁	F₂	F₃	F₄	F₅	F₆	F_综	排名 Ranking
S1	-0.17	2.69	2.69	-0.91	-1.39	0.75	0.74	13
S2	-6.93	0.26	0.53	-0.88	2.15	-0.37	-2.43	30
S3	-0.83	-1.76	2.15	0.91	1.05	1.73	-0.17	19
S4	14.42	4.13	-2.11	0.39	-0.76	1.44	6.29	1
S5	-5.06	8.44	1.35	0.26	0.54	-0.70	0.29	16
S6	-5.80	-0.96	-2.29	-0.62	-0.38	-1.05	-2.88	32
S7	5.20	-4.11	1.91	-0.80	-1.25	-1.20	0.94	10
S8	-2.75	2.01	-0.19	-0.05	0.63	0.44	-0.50	23
S9	-2.91	-1.91	-1.33	-0.07	-1.58	1.73	-1.75	28
S10	-2.08	0.24	1.35	-1.47	-0.82	0.53	-0.76	25
S11	2.40	1.45	0.26	-0.30	-1.93	-2.38	0.92	11
S12	0.52	-10.54	0.25	1.37	-0.55	-0.23	-2.23	29
S13	5.27	-1.18	0.17	0.41	-0.78	-1.12	1.63	6
S14	5.18	0.85	-0.66	0.01	0.29	1.93	2.26	5
S15	-8.74	-3.52	-1.29	-1.64	0.29	-0.65	-4.51	35
S16	-6.12	-1.22	-1.85	3.05	-1.42	0.84	-2.61	31
S17	2.94	-5.68	0.55	-0.48	-1.43	-0.13	-0.36	21
S18	0.83	-0.11	0.34	1.31	-0.71	0.17	0.41	15
S19	0.76	5.52	-0.90	0.35	-1.00	-0.55	1.41	8
S20	-1.87	0.38	1.54	-1.32	-0.42	1.56	-0.50	22
S21	-1.03	1.34	-1.30	-1.48	1.59	-0.67	-0.28	20
S22	4.33	0.96	4.05	4.05	1.63	-1.16	2.83	3
S23	5.32	2.13	1.11	-1.24	-0.22	-1.30	2.43	4
S24	-2.38	3.87	-0.24	0.52	0.38	0.11	0.09	18
S25	-3.53	0.98	-0.19	-2.96	-0.43	0.19	-1.45	27
S26	2.57	1.05	-0.45	-0.66	-2.44	-0.67	0.84	12
S27	2.00	0.76	1.32	-1.58	0.93	1.19	1.11	9
S28	5.91	-2.45	-1.21	-1.69	2.86	-1.02	1.53	7
S29	2.65	-0.94	-0.67	-0.14	-0.86	0.51	0.64	14
S30	-8.88	0.09	1.30	1.72	-0.42	-0.03	-3.07	34
S31	-7.73	-1.04	1.33	0.14	0.73	0.44	-2.92	33
S32	-1.05	-2.64	-0.57	-0.08	2.17	-0.14	-0.92	26
S33	-1.95	1.14	-3.77	1.41	0.46	-0.26	-0.76	24
S34	-0.35	1.50	-2.54	2.41	0.51	-0.48	0.18	17
S35	9.88	-1.71	-0.66	0.05	2.57	0.55	3.54	2

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