套袋对桃果实发育后期果皮类胡萝卜素的影响

颜少宾; 金光; 张妤艳; 马瑞娟; 俞明亮

doi:10.19303/j.issn.1008-0384.2019.07.006

套袋对桃果实发育后期果皮类胡萝卜素的影响

doi: 10.19303/j.issn.1008-0384.2019.07.006

颜少宾^{1, 2,},
金光¹,
张妤艳²,
马瑞娟²,
俞明亮^2, ,

1.
福建省农业科学院果树研究所, 福建福州 350013
2.
江苏省农业科学院果树研究所, 江苏南京 210014

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项 2017R1013-7

国家现代农业产业技术体系建设专项 CARS-30-Z-07

福建省财政专项——福建省农业科学院创新团队建设项目 STIT2017-1-4

详细信息

作者简介:
颜少宾(1988-), 女, 硕士, 助理研究员, 主要从事桃种质资源和果实品质研究(E-mail:ysb2010_good@163.com)

通讯作者:
俞明亮(1965-), 男, 博士, 研究员, 主要从事桃种质资源和育种研究(E-mail:mly1008@aliyun.com)

中图分类号: S662.1
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出版历程
- 收稿日期: 2018-06-05
- 修回日期: 2019-03-20
- 刊出日期: 2019-07-20

Effect of Bagging on Carotenoids in Peels of Peaches at Maturing Stages

1.
Fruit Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
2.
Fruit Institute, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China

摘要

摘要: 目的为了提高黄肉桃果实的外观品质，通过合理套袋科学指导黄肉桃生产栽培。方法以图八德黄肉桃为试材，于盛花后50 d分别进行黄色单层袋、外黄内黑双层袋果实套袋，以不套袋处理为对照。盛花后98、105、110 d测定图八德果实发育后期果实的单果重、果皮色差、类胡萝卜素含量的变化。结果两个套袋处理均显著提高了图八德果皮的光泽度和色彩鲜艳度，但对果实大小没有显著影响。与对照相比，黄色单层袋处理使果皮叶黄素含量在盛花后98 d显著升高，105 d时显著降低；而外黄内黑双层袋处理显著降低果皮叶黄素含量；果实成熟时，其含量均接近0。黄色单层袋处理显著降低了果皮玉米黄素的含量；在盛花后98 d时，外黄内黑双层袋处理对玉米黄素的含量没有影响，而在盛花后105、110 d时，显著降低玉米黄素的含量。两个套袋处理对果皮β-隐黄质含量的影响不明显。黄色单层袋处理对α-胡萝卜素含量的影响不明显；外黄内黑双层袋处理显著提高α-胡萝卜素含量。两个套袋处理的β-胡萝卜素的含量在盛花后98、105 d与CK差异不显著（P>0.05）；果实成熟时，两个套袋处理均显著提高了果皮β-胡萝卜素的含量。尤其是外黄内黑双层袋处理后，果皮β-胡萝卜素的含量提高了6.69 mg·hg^-1。结论建议在田间栽培管理上，对黄肉桃进行外黄内黑双层袋套袋栽培，可提高黄肉桃果皮光泽度和色彩鲜艳度以及α-胡萝卜素、β-胡萝卜素含量。
- 桃 /
- 套袋 /
- 类胡萝卜素 /
- 单果重 /
- 色差
Abstract: Objective To enhance the coloration of yellow peaches by bagging the fruits on the trees. Method Yellow single-layer or yellow-and-black two-layer paper pouches were used to cover the Tubade peaches 50 d after full bloom, in comparison with no bagging as CK. The individual fruit weight as well as the chromatic aberration and carotenoids content of peel were measured in the late peach developmental stages. Result Either of the applied bagging material could enrich the glossiness and saturation of the peels with no effect on fruit weight. The yellow pouches did not exert a consistent effect on xanthophylls, whereas, the two-layer pouches significantly reduced the content in the peels as compared to CK. Upon ripening, the xanthophylls contents of all treatments were close to 0. The yellow pouches significantly reduced the zeaxanthins, whereas, the two-layer pouches showed no effect on it 48 d but significantly reduced the content 55 d and 60 d after bagging. There was no significant effect on β-cryptoxanthin by the bagging or on α-carotene in peels of the fruits covered by the yellow pouches. However, the two-layer pouches significantly increased the α-carotene content than CK. Both bagging treatments significantly raised the β-carotene content in peels of the ripe fruits but not significantly (P>0.05) as compared with CK on 48 d or 55 d after the treatments, which was 6.69 mg·hg^-1 higher than CK with two-layer treatment at riping stage. Conclusion Bagging the peaches on trees cultivated on an orchard with the yellow-outside-and-black-inside paper pouches could increase glossiness and as well as the contents of α-carotene and β-carotene on the peels improving the appearance of the fruits.
- peach /
- bagging /
- carotenoids /
- fruit weight /
- chromatic aberration

HTML全文

图 1 套袋对图八德果实发育后期单果重的影响

注：CK为对照，即不套袋处理; Ⅰ为套黄色单层袋处理，Ⅱ为套外黄内黑双层袋处理。不同字母表示在同一发育阶段中不同处理条件下差异显著(P < 0.05)。表 1、图 2、图 3同。

Figure 1. Effect of bagging on fruit weight of Tubade peaches in later developmental stages

Note:CK indicates no bagging for comparison with treatments; Ⅰ.yellow single-layer pouches; Ⅱ.yellow-black two-layer pouches. Data with different letters in a same row represent significant difference at 0.05 level among different treatments in a same developmental stage.The same as Table 2, Fig. 2, Fig. 3.

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图 2 不同套袋对图八德果实发育后期果皮颜色的影响

Figure 2. Effect of bagging on color of Tubade peach peels in later developmental stage

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图 3 套袋对图八德果实发育后期果皮类胡萝卜素成分的影响

Figure 3. Effect of bagging on carotenoids content of Tubade peach peels in later developmental stage

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表 1 不同套袋对图八德果皮色差的影响

Table 1. Effect of bagging on chromatic aberration of Tubade peach peels in same developmental stage

盛花后天数 Days after full bloom/d	处理 Treatment	色差a值 Chromatism a value	色差b值 Chromatism b value	色差c值 Chromatism c value	色差L值 Chromatism L value	色差h值 Chromatism h value
98	CK	15.51±3.37a	26.45±4.47b	32.12±2.93b	59.27±7.81b	48.95±4.19b
	I	6.66±3.00b	38.25±1.87a	39.12±1.76a	80.35±4.24a	60.23±1.80a
	Ⅱ	7.13±1.77b	40.39±1.91a	41.12±2.14a	80.13±2.09a	61.84±1.68a
105	CK	18.17±0.87a	27.74±5.98c	34.20±4.67c	56.76±6.29b	50.04±4.43c
	I	13.14±2.41b	37.86±2.33b	40.50±2.31b	71.17±3.32a	56.75±2.94b
	Ⅱ	11.64±1.72b	43.54±1.58a	45.18±1.72a	75.15±2.07a	60.31±0.94a
110	CK	20.83±1.64a	26.22±2.56c	34.21±2.19c	49.64±3.38c	50.21±2.58c
	I	16.07±4.63b	40.34±3.35b	44.06±1.84b	67.58±7.02b	61.65±3.19b
	Ⅱ	13.64±2.28b	44.05±2.55a	46.21±2.93a	72.77±2.32a	64.23±1.95a
注：不同字母表示同一个品种在同一发育阶段中不同处理条件下在0.05水平差异显著。色差a值代表红色饱和度(正值代表红色，正值越大，红色越深)；色差b值代表黄色饱和度(正值代表黄色，正值越大，黄色越深)；色差L值代表亮度值；色差c值代表色彩饱和度；色差h值代表色度角(h=0，紫红色；h=90，黄色；h=180，绿色)。 Note：With different letters in a same row represent significant difference at 0.05 level among different treatments in a same developmental stage. Chromatism a represents saturation of red color, the higher positive value represents deeper red; Chromatism b, saturation of yellow color, the higher positive value, the deeper the color; L, brightness; c, color saturation; and, h, hue (h=0 reddish purple; h=90, yellow; and, h=180, green).

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参考文献(25)

[1]	MILI W. Biological functions and activities of animal carotenoids[J]. Pure Appl Chem, 1991, 63:141-146. doi: 10.1351/pac199163010141
[2]	NAGAO A. Absorption and function of dietary carotenoids[J]. Forum Nutr, 2009, 61:55-63. doi: 10.1159/000212738
[3]	FRASER P D, BRAMLEY P M. The biosynthesis and nutritional uses of carotenoids[J]. Prog Lipid Res, 2004, 43(3):228-265. doi: 10.1016/j.plipres.2003.10.002
[4]	NISHINO H, MURAKOSHI M, TOKUDA H, et al. Cancerprevention by carotenoids[J]. Arch Biochem Biophys, 2009, 483(2):65-168.
[5]	ITO M, YAMANO Y, TODE C, et al. Carotenoid synthesis:retrospect and recent progress[J]. Arch Biochem Biophys, 2009, 483(2):224-228. doi: 10.1016/j.abb.2008.11.021
[6]	糜林, 王全洪, 费宪进, 等.优质水蜜桃品种高效套袋技术研究[J].江苏农业科学, 2007(5):106-108. doi: 10.3969/j.issn.1002-1302.2007.05.036 MI L, WANG Q H, FEI X J, et al. Study the efficient bagging technology of high quality peach varieties[J].Jiangsu Agricultural Sciences, 2007(5):106-108.(in Chinese) doi: 10.3969/j.issn.1002-1302.2007.05.036
[7]	马瑞娟, 张斌斌, 蔡志翔, 等.不同类型果袋对霞光油桃果实品质的影响[J].江苏农业学报, 2012, 28(3):627-631. doi: 10.3969/j.issn.1000-4440.2012.03.032 MA R J, ZHAGN B B, CAI Z X, et al. Effect of different bags on fruit quality of Xiaguang nectarine[J]. Jiangsu Journal of Agricultural Sciences, 2012, 28(3):627-631.(in Chinese) doi: 10.3969/j.issn.1000-4440.2012.03.032
[8]	李兴国.黑穗醋栗果实中花青苷合成与PAL活性的关系[J].吉林农业大学学报, 2009, 31(5):603-606. http://d.old.wanfangdata.com.cn/Periodical/jlnydxxb200905029 LI X G. Relationship between Anthocyanin Syuthesis and PAL Activity in Fruits of Blackcurrant[J]. Journal of Jilin Agricultural University, 2009, 31(5):603-606.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jlnydxxb200905029
[9]	王少敏, 高华君, 张骁兵.套袋对红富士苹果色素及糖、酸含量的影响[J].园艺学报, 2002, 29(3):263-265. doi: 10.3321/j.issn:0513-353X.2002.03.016 WANG S M, GAO H J, ZHANG Q B, et al. Effects of bagging on pigment, sugar and acid development in 'Red Fuji' Apple Fruits[J]. Acta Horticulturae Sinica, 2002, 29(3):263-265.(in Chinese) doi: 10.3321/j.issn:0513-353X.2002.03.016
[10]	王安柱, 韩明玉, 丁勤, 等.不同类型果袋对秦光2号油桃品质的影响[J].果树学报, 2006, 23(4):602-604. http://d.old.wanfangdata.com.cn/Periodical/gskx200604025 WAGN A Z, HAN M Y, DING Q, et al. Effect of different bags on the quality of Qinguang 2 nectarine[J].Journal of Fruit Science, 2006, 23(4):602-604.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/gskx200604025
[11]	陈栋, 谢红江, 李靖, 等.套袋对桃果实品质形成和果皮色素变化规律的影响[J].西南农业学报, 2011, 24(6):2132-2136. doi: 10.3969/j.issn.1001-4829.2011.06.022 CHEN D, XIE H J, LI J, et al. Effects of bagging on fruit quality and variation of skin pigment of peach[J]. Southwest China Journal of Agricultural Sciences, 2011, 24(6):2132-2136.(in Chinese) doi: 10.3969/j.issn.1001-4829.2011.06.022
[12]	颜少宾, 蔡志翔, 俞明亮, 等.桃果实发育阶段肉色形成与类胡萝卜素的变化分析[J].西北植物学报, 2013, 33(3):613-619. doi: 10.3969/j.issn.1000-4025.2013.03.027 YAN S B, CAI Z X, YU M L, et al. Incarnadine formation and carotenoids changes in peach fruit at different development stages[J].Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(3):613-619.(in Chinese) doi: 10.3969/j.issn.1000-4025.2013.03.027
[13]	颜少宾, 张妤艳, 马瑞娟, 等.桃果实类胡萝卜素测定方法的研究[J].果树学报, 2012, 29(6):1127-1133. http://www.cnki.com.cn/Article/CJFDTotal-GSKK201206031.htm YAN S B, ZHANG Y Y, MA R J, et al. Study the measurement of carotenoids from the peach fruit[J]. Journal of Fruit Science, 2012, 29(6):1127-1133.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-GSKK201206031.htm
[14]	王安柱, 韩明玉, 丁秦, 等.不同类型果袋对秦王桃品质的影响[J].西北林学院学报, 2007, 22(1):78-80. doi: 10.3969/j.issn.1001-7461.2007.01.021 WANG A Z, HAN M Y, DING Q, et al. Effect of different bags on the quality of Qinwang peach[J].Journal of Northwest Forestry University, 2007, 22(1):78-80.(in Chinese) doi: 10.3969/j.issn.1001-7461.2007.01.021
[15]	LI B, JIA H J, ZHANG X M. Effects of Fruit Pre-harvest Bagging on Fruit Quality of Peach (Prunus persica Batsch cv. Hujingmilu)[J]. Journal of Plant Physiology and Molecular Biology, 2006, 32(3):250-286. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zwslxb200603003
[16]	郭宝林, 鲁韧强.套袋提高燕红桃果品质量的试验研究[J].中国果树, 2000(3):19-20. doi: 10.3969/j.issn.1003-0034.2000.03.007 GUO B L, LU R Q. Experimental study on improving the quality of Yanhong peach fruit by bagging[J]. China Fruits, 2000(3):19-20.(in Chinese) doi: 10.3969/j.issn.1003-0034.2000.03.007
[17]	沈玉英, 李斌, 贾惠娟.不同纸质果袋对湖景蜜露桃果实品质的影响[J].果树学报, 2006, 23(2):182-185. http://d.old.wanfangdata.com.cn/Periodical/gskx200602007 SHEN Y Y, LI B, JIA H J. Effect of bagging on fruit quality of Hujingmilu peach cultivar[J]. Journal of Fruit Science, 2006, 23(2):182-185.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/gskx200602007
[18]	张学英.李果实着色与花色素苷合成机理研究[D].杭州: 浙江大学, 2008. ZHANG X Y. Research on pigmentation and mechanism of anthocyanin synthesis in plum (Prunus spp.) fruits[D]. Hangzhou: Zhejiang University, 2008.(in Chinese)
[19]	马瑞娟, 张斌斌, 张春华, 等.套袋对金陵黄露桃果实品质的影响[J].江苏农业学报, 2014, 30(5):1127-1131. doi: 10.3969/j.issn.1000-4440.2014.05.031 MA R J, ZHANG B B, ZHANG C H, et al. Effect of bagging on quality of Jinlinghuanglu peach[J]. Jiangsu Journal of Agricultural Sciences, 2014, 30(5):1127-1131.(in Chinese) doi: 10.3969/j.issn.1000-4440.2014.05.031
[20]	陈巍, 黄品湖, 郭秀珠, 等.套袋对油桃果皮色素和果实品质的影响[J].浙江农业科学, 2014(11):1705-1706. doi: 10.3969/j.issn.0528-9017.2014.11.018 CHEN W, HUANG P H, GUO X Z, et al. Effect of bagging on pigment and quality of nectarine fruit[J]. Journal of Zhejiang Agricultural Sciences, 2014(11):1705-1706.(in Chinese) doi: 10.3969/j.issn.0528-9017.2014.11.018
[21]	陈军, 高文胜, 吕德国, 等.套袋红富士苹果果皮发育进程研究[J].果树学报, 2009, 26(1):17-21. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gskx200902018 CHEN J, GAO W S, LV D G, et al. The effects of bagging on the fruit skin development process of Red Fuji apple[J]. Journal of Fruit Science, 2009, 26(1):17-21.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gskx200902018
[22]	梁敏华.桃果实中类胡萝卜素合成基因的克隆及其对蓝光的响应机制研究[D].上海: 上海海洋大学, 2015. http://cdmd.cnki.com.cn/Article/CDMD-10264-1015975157.htm LIANG M H. Molecular cloning and expression of carotenogenic genes which responses to blue light in peach fruit[D].Shanghai: Shanghai Ocean University, 2015.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10264-1015975157.htm
[23]	冯唐锴, 李思光, 罗玉萍, 等.植物β-胡萝卜素羟化酶研究进展[J].生物技术通报, 2007(1):54-58. doi: 10.3969/j.issn.1002-5464.2007.01.013 FENG T K, LI S G, LUO Y P, et al. Research progress on β-carotene hydroxylase of plant[J]. Biotechnology Bulletin, 2007(1):54-58.(in Chinese) doi: 10.3969/j.issn.1002-5464.2007.01.013
[24]	董新甜, 曹洪波, 张飞燕, 等.遮光性套袋对黄肉桃类胡萝卜素合成及相关基因表达的影响[J].园艺学报, 2015, 42(4):633-642. http://d.old.wanfangdata.com.cn/Periodical/zgyywz201607108 DONG X T, CAO H B, ZHANG F Y, et al. Effects of shading fruit with opaque paper bag on carotenogenesis and related gene expression in yellow-flesh peach[J]. Acta Horticulturae Sinica, 2015, 42(4):633-642.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgyywz201607108
[25]	MC ELROY J S, KOPSELL D A, SOROCHAN J C, et al. Response of creeping bentgrass carotenoid composition to high and low irradiance[J]. Crop Science, 2006, 46:2606-2612. doi: 10.2135/cropsci2006.02.0119