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柑橘果实响应高温、强光胁迫的活性氧代谢研究

万继锋 李娟 杨为海 曾辉 张汉周 陈杰忠

万继锋, 李娟, 杨为海, 曾辉, 张汉周, 陈杰忠. 柑橘果实响应高温、强光胁迫的活性氧代谢研究[J]. 福建农业学报, 2019, 34(8): 920-924. doi: 10.19303/j.issn.1008-0384.2019.08.008
引用本文: 万继锋, 李娟, 杨为海, 曾辉, 张汉周, 陈杰忠. 柑橘果实响应高温、强光胁迫的活性氧代谢研究[J]. 福建农业学报, 2019, 34(8): 920-924. doi: 10.19303/j.issn.1008-0384.2019.08.008
WAN Ji-feng, LI Juan, YANG Wei-hai, ZENG Hui, ZHANG Han-zhou, CHEN Jie-zhong. ROS Metabolism of Citrus Fruits in Response to High-temperature-intense-light Stress[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 920-924. doi: 10.19303/j.issn.1008-0384.2019.08.008
Citation: WAN Ji-feng, LI Juan, YANG Wei-hai, ZENG Hui, ZHANG Han-zhou, CHEN Jie-zhong. ROS Metabolism of Citrus Fruits in Response to High-temperature-intense-light Stress[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 920-924. doi: 10.19303/j.issn.1008-0384.2019.08.008

柑橘果实响应高温、强光胁迫的活性氧代谢研究

doi: 10.19303/j.issn.1008-0384.2019.08.008
基金项目: 

国家自然科学基金项目 31372008

广东省科技计划项目 2014A020209081

广东省科技计划项目 2014A020208085

广东省科技计划项目 2015A020209110

详细信息
    作者简介:

    万继锋(1983—), 男, 硕士, 助理研究员, 研究方向:果树种质资源与栽培育种(E-mail:wanjifeng2002@163.com)

    通讯作者:

    陈杰忠(1958—), 男, 教授, 研究方向:南方果树的栽培生理研究与教学(E-mail:cjzlxb@scau.edu.cn)

  • 中图分类号: S666

ROS Metabolism of Citrus Fruits in Response to High-temperature-intense-light Stress

  • 摘要:   目的  研究柑橘果实响应高温、强光胁迫的活性氧代谢,为预防柑橘果实发生日灼以及果品优质生产提供依据。  方法  在环境调控生长室中模拟高温、强光诱导柑橘果实发生灼伤,并从果实灼伤病程的不同阶段入手,分析了果实灼伤病程中果皮活性氧代谢的变化规律。  结果  高温、强光胁迫使果皮中O2·-大量积累,LOX活性增强,脂质过氧化产物MDA含量显著上升。灼伤初期果皮呈现深黄色小斑点,SOD、POD和PPO活性均显著上升;随着灼伤病情的加剧,果皮变褐,POD活性仍增强,SOD活性显著下降且显著低于正常果,而PPO活性显著下降,但仍显著高于正常果;AsA和GSH含量在整个灼伤病程中均逐步下降。  结论  在高温、强光胁迫初期果皮呈现深黄色小斑点,柑橘果实果皮组织维持较高的活性氧清除能力,减缓了高温、强光对细胞膜的损伤。果皮呈现深黄色小斑点是预防柑橘果实果皮褐变的重要时间节点。
  • 图  1  诱导柑橘果实灼伤病程中果皮细胞的膜脂过氧化

    注:Ⅰ为正常果,Ⅱ为斑点果,Ⅲ为轻度褐变果,Ⅳ为重度褐变果。图中不同小写字母表示差异显著(P < 0.05)。图 2~3同。

    Figure  1.  Changes in cell membrane lipid peroxidation of Citrus Fruits under high-temperature-intense-light stress

    Note:Ⅰ.normal fruit, Ⅱ.dark yellow spots fruit, Ⅲ.slight browning fruit, Ⅳ.serious browning fruit.Different lowercase letters in the figure indicated significant differences (P < 0.05).The same as Fig. 2-3.

    图  2  诱导柑橘果实灼伤病程中果实抗氧化酶变化

    Figure  2.  Changes in antioxidant enzymes of Citrus Fruits under high-temperature-intense-light stress

    图  3  诱导柑橘果实灼伤病程中果皮抗氧化剂的变化

    Figure  3.  Changes in antioxidants of Citrus Fruits under high-temperature-intense-light stress

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  • 收稿日期:  2019-06-24
  • 修回日期:  2019-07-15
  • 刊出日期:  2019-08-01

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