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半番鸭不同组织Cu/Zn-SOD基因表达及酶活性研究

郑嫩珠 章琳俐 李丽 辛清武 缪中纬 朱志明 黄一帆

郑嫩珠, 章琳俐, 李丽, 辛清武, 缪中纬, 朱志明, 黄一帆. 半番鸭不同组织Cu/Zn-SOD基因表达及酶活性研究[J]. 福建农业学报, 2019, 34(8): 933-938. doi: 10.19303/j.issn.1008-0384.2019.08.010
引用本文: 郑嫩珠, 章琳俐, 李丽, 辛清武, 缪中纬, 朱志明, 黄一帆. 半番鸭不同组织Cu/Zn-SOD基因表达及酶活性研究[J]. 福建农业学报, 2019, 34(8): 933-938. doi: 10.19303/j.issn.1008-0384.2019.08.010
ZHENG Nen-zhu, ZHANG Lin-li, LI Li, XIN Qing-wu, MIAO Zhong-wei, ZHU Zhi-ming, HUANG Yi-fan. Expression of Cu/Zn-SOD Gene and Activity of Cu/Zn-SOD Enzyme in Mule Duck Tissues[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 933-938. doi: 10.19303/j.issn.1008-0384.2019.08.010
Citation: ZHENG Nen-zhu, ZHANG Lin-li, LI Li, XIN Qing-wu, MIAO Zhong-wei, ZHU Zhi-ming, HUANG Yi-fan. Expression of Cu/Zn-SOD Gene and Activity of Cu/Zn-SOD Enzyme in Mule Duck Tissues[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 933-938. doi: 10.19303/j.issn.1008-0384.2019.08.010

半番鸭不同组织Cu/Zn-SOD基因表达及酶活性研究

doi: 10.19303/j.issn.1008-0384.2019.08.010
基金项目: 

中央引导地方科技发展专项 2018L3004

福建省发改委"五新"项目 016032018001

福建省财政专项——福建省农业科学院创新团队建设项目 STIT2017-2-1

详细信息
    作者简介:

    郑嫩珠(1972-), 女, 博士, 研究员, 研究方向:畜禽遗传育种与农产品加工(E-mail:hql202@126.com)

    通讯作者:

    黄一帆(1954-), 男, 教授, 博士生导师, 研究方向:农产品加工与贮藏(E-mail:zjhyfang@163.com)

  • 中图分类号: X131;X53

Expression of Cu/Zn-SOD Gene and Activity of Cu/Zn-SOD Enzyme in Mule Duck Tissues

  • 摘要:   目的  首次对半番鸭不同组织Cu/Zn-SOD基因表达、酶活性进行研究,以期了解半番鸭不同组织抗氧化特性,为深入研究半番鸭对环境因子的响应能力及相关分子机制提供参考。  方法  通过实时荧光定量法、黄嘌呤氧化酶法和硫代巴比妥酸法分别测定了半番鸭心脏、肝脏、脾脏、肺脏、肾脏、肌胃和胸肌中的Cu/Zn-SOD mRNA相对表达量、酶活力和MDA含量。  结果  Cu/Zn-SOD mRNA在胸肌中相对表达量最高,总体趋势为胸肌>肝脏>肺>心脏>脾脏>肾脏/肌胃。酶活力测定结果显示,肝脏和肾脏Cu/Zn-SOD酶活力显著高于胸肌和肌胃。半番鸭胸肌MDA含量显著高于肝脏、肌胃和肾脏,提示胸肌可能更易受到氧化应激损伤。相关分析结果显示Cu/Zn-SOD活力与MDA含量呈显著负相关,而抗氧化酶活力与mRNA表达水平相关性不显著。  结论  半番鸭Cu/Zn-SOD基因表达和酶活力具有组织特异性。不同组织Cu/Zn-SOD活力差异可能与Cu/Zn-SOD自身的生理功能以及组织代谢水平有关。
  • 图  1  目的基因qRT-PCR扩增曲线和熔解曲线

    注:A为SOD1扩增曲线(左)和熔解曲线(右),B为β-actin基因扩增曲线(左)和熔解曲线(右)。

    Figure  1.  Amplification and melting curves of qRT-PCR on target genes

    Note:A: SOD1 gene amplification curve (left) and melting curve (right); B: β-actin amplification curve (left) and melting curve (right).

    图  2  半番鸭不同组织Cu/Zn-SOD mRNA的相对表达

    注:1:心脏;2:肝脏;3:脾脏;4:肺脏;5:胸肌;6:肌胃;7:肾脏。不同小写字母表示在P<0.05水平差异有统计学意义,不同大写字母表示在P<0.01水平差异有高度统计学意义。

    Figure  2.  Relative expressions of Cu/Zn-SOD mRNA in mule duck tissues

    Note: 1: heart; 2: liver; 3: spleen; 4: lung; 5: chest; 6: gizzard; and, 7: kidney. Data with different lowercase letters indicate significant differences at P < 0.05; and, data with different capital letters indicate significant differences at P < 0.01.

    图  3  抗氧化酶活力与mRNA、MDA含量相关性分析

    Figure  3.  Correlation coefficients between antioxidant enzyme activity and mRNA expression/MDA content

    表  1  不同时期饲料的营养水平

    Table  1.   Nutrients in diets for mule ducks at various stages

    营养水平
    Nutrient levels/%
    0~21 日龄
    0~21 days of age
    22~42 日龄
    22~42 days of age
    43~70 日龄
    43~70 days of age
    粗蛋白 CP ≥18.0 ≥16.0 ≥15.0
    粗纤维 CF ≤5.0 ≤6.0 ≤7.0
    粗灰分 CA ≤8.0 ≤12.0 ≤12.0
    钙 Ca 0.70~1.40 0.70~1.40 0.70~1.40
    总磷 AP ≥0.40~0.80 ≥0.35~0.75 ≥0.35~0.75
    食盐 NaCl 0.30~0.80 0.30~0.80 0.30~0.80
    水分 Water ≤13.0 ≤13.0 ≤13.0
    下载: 导出CSV

    表  2  荧光定量PCR引物信息

    Table  2.   Information on qRT-PCR primers

    基因
    Genes
    登录号
    GenBank No.
    引物序列(5′→3′)
    Primer Sequences (5′to 3′)
    扩增长度
    Size/bp
    Tm
    /℃
    SOD1 KU048808.1 F: CAGCAGGGTAACGGACCAGTAAA;R: CCCAAATTCGTGGACATGGAAG 87 60
    ACTB EF667345 F: GATGTGGATCAGCAAGCAGGAGT;R: GGGTGTGGGTGTTGGTAACAGT 95 60
    注:SOD1:铜锌超氧化物歧化酶;ACTB:β-肌动蛋白。
    Note: SOD1: Cu/Zn SOD; ACTB: β-actin.
    下载: 导出CSV

    表  3  半番鸭不同组织Cu/Zn-SOD活力和MDA含量

    Table  3.   Cu/Zn-SOD enzyme activity and MDA content in mule duck tissues

    指标
    Index
    肝脏
    Liver
    胸肌
    Chest muscle
    肌胃
    Gizzard
    肾脏
    Kidney
    Cu/Zn-SOD活力Cu/Zn-SOD activity/(U·mg-1) 176.07±4.57Aa 39.37 ±1.41Cc 48.49±2.08Cc 117.64±4.85 Bb
    MDA含量MDA content/(n mol·mg-1) 1.07±0.02Ba 2.84±0.18 Aa 1.10±0.03 Bb 0.89±0.02 Cc
    注:同行数据后不同小写字母表示差异显著(P<0.05),不同大写字母表示差异极显著(P<0.01)。
    Note: In the same row, different lowercase letters represent statistically significant differences at the 0.05 probability level; different capital letters represent highly statistically significant differences at the 0.01 probability level.
    下载: 导出CSV
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  • 收稿日期:  2019-07-10
  • 修回日期:  2019-08-03
  • 刊出日期:  2019-08-01

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