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建阳桔柚两种真菌病原菌的鉴定

葛慈斌 黄素芳 阮传清 肖荣凤 刘韬

葛慈斌, 黄素芳, 阮传清, 肖荣凤, 刘韬. 建阳桔柚两种真菌病原菌的鉴定[J]. 福建农业学报, 2019, 34(8): 958-964. doi: 10.19303/j.issn.1008-0384.2019.08.014
引用本文: 葛慈斌, 黄素芳, 阮传清, 肖荣凤, 刘韬. 建阳桔柚两种真菌病原菌的鉴定[J]. 福建农业学报, 2019, 34(8): 958-964. doi: 10.19303/j.issn.1008-0384.2019.08.014
GE Ci-bin, HUANG Su-fang, RUAN Chuan-qing, XIAO Rong-feng, LIU Tao. Pathogens of Diseases on Jianyang Tangelos[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 958-964. doi: 10.19303/j.issn.1008-0384.2019.08.014
Citation: GE Ci-bin, HUANG Su-fang, RUAN Chuan-qing, XIAO Rong-feng, LIU Tao. Pathogens of Diseases on Jianyang Tangelos[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 958-964. doi: 10.19303/j.issn.1008-0384.2019.08.014

建阳桔柚两种真菌病原菌的鉴定

doi: 10.19303/j.issn.1008-0384.2019.08.014
基金项目: 

福建省农业科技跨越计划项目 2005-14

福建省财政专项——福建省农业科学院科技创新团队建设项目 STIT2017-1-11

详细信息
    作者简介:

    葛慈斌(1973-), 男, 硕士, 副研究员, 研究方向:农业微生物(E-mail:benci2000@sohu.com)

    通讯作者:

    刘韬(1962-), 男, 教授级高级农艺师, 研究方向:果树学、生态农业(E-mail:liutao1202@sina.com)

  • 中图分类号: S687

Pathogens of Diseases on Jianyang Tangelos

  • 摘要:   目的  明确引起福建建阳桔柚落叶、枝梢枯死的两种病害的病原菌种类,为防治病害提供基础。  方法  从建阳桔柚上采集典型发病叶片,进行病原菌的分离、形态观察和菌株rDNA-ITS区序列比对、致病性测定。  结果  桔柚病叶片中分离出两种主要病原真菌,代表菌株分别为FJAT-32214和FJAT-32151。菌株FJAT-32214在PSA培养基上的菌落颜色为灰褐色,产褐色分生孢子,多呈链状分隔,其rDNA-ITS区序列与互隔交链孢霉Alternaria alternata的ITS序列相似性达99%;菌株FJAT-32151在PSA培养基平板上的菌丝呈灰白色,绒状、茂密,后期会在菌落表面产生砖红色孢子堆,分生孢子柱形或棒状,单胞,无色,其rDNA-ITS区序列与胶孢炭疽菌Colletotrichum gloeosporioides的ITS序列相似性也达99%,通过回接可引起样本致病,分离获得与接种物一致的病原物,符合Koch氏法则。  结论  通过病原形态学与ITS序列分析,鉴定了发生在建阳桔柚叶片上的两种主要真菌性病原,分别为引起褐斑病的互隔交链孢霉和炭疽病的胶孢炭疽菌。
  • 图  1  褐斑病叶片症状

    Figure  1.  Symptoms of on a tangelo leaf

    图  2  炭疽病叶片症状

    注:A为病叶整体; B为病叶的局部。

    Figure  2.  Symptoms of anthracnose disease on a tangelo leaf

    Note:A: the whole sick leaves; B:part of sick leaf.

    图  3  菌株FJAT-32214的菌落形态

    注:A为正面,B为背面。图 5同。

    Figure  3.  Morphology of FJAT-32214 colonies

    Note:A:faade side, B:reverse side.The same as Fig. 5.

    图  4  菌株FJAT-32214的分生孢子器及分生孢子

    Figure  4.  Pycnidium and spores of FJAT-32214

    图  5  菌株FJAT-32151的菌落形态

    Figure  5.  Morphology of FJAT-32151 colonies

    图  6  菌株FJAT-32151的分生孢子及分生孢子器

    注:A为分生孢子,B为分生孢子器。

    Figure  6.  Spores and pycnidium of strain FJAT-32151

    Note:A:spores, B:pycnidium.

    图  7  菌株FJAT-32214基于ITS序列构建的分子系统发育树

    Figure  7.  Phylogenetic dendrogram of strain FJAT-32214 and other 11 Alternaria sp. strains based on ITS sequences

    图  8  菌株FJAT-32151基于ITS序列构建的分子系统发育树

    Figure  8.  Phylogenetic dendrogram of strain FJAT-32151 and other 11 Colletotrichum sp. strains based on ITS sequences

    图  9  回接后发病的褐斑病症状

    Figure  9.  Symptoms of brown spot on leaf after pathogen inoculation

    图  10  回接后发病的炭疽病症状

    Figure  10.  Symptoms of anthracnose on leaf after pathogen inoculation

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  • 收稿日期:  2019-07-15
  • 修回日期:  2019-08-24
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