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春兰‘黄梅’ב黄荷’杂交F1根状茎的组培快繁研究

漆子钰 周育真 艾叶 彭东辉

漆子钰,周育真,艾叶,等. 春兰‘黄梅’ב黄荷’杂交F1根状茎的组培快繁研究 [J]. 福建农业学报,2019,34(9):1032−1039. doi: 10.19303/j.issn.1008-0384.2019.09.006
引用本文: 漆子钰,周育真,艾叶,等. 春兰‘黄梅’ב黄荷’杂交F1根状茎的组培快繁研究 [J]. 福建农业学报,2019,34(9):1032−1039. doi: 10.19303/j.issn.1008-0384.2019.09.006
QI Z Y, ZHOU Y Z, AI Y, et al. Tissue Culture for Rapid Propagation of A Cymbidium goeringii Hybrid [J]. Fujian Journal of Agricultural Sciences,2019,34(9):1032−1039. doi: 10.19303/j.issn.1008-0384.2019.09.006
Citation: QI Z Y, ZHOU Y Z, AI Y, et al. Tissue Culture for Rapid Propagation of A Cymbidium goeringii Hybrid [J]. Fujian Journal of Agricultural Sciences,2019,34(9):1032−1039. doi: 10.19303/j.issn.1008-0384.2019.09.006

春兰‘黄梅’ב黄荷’杂交F1根状茎的组培快繁研究

doi: 10.19303/j.issn.1008-0384.2019.09.006
基金项目: 福建省种业创新与产业化工程林业项目(ZYCX-LY-2017005)
详细信息
    作者简介:

    漆子钰(1992−),女,硕士,助理工程师,研究方向:园林植物与应用研究(E-mail:qiziyu2013@126.com

    通讯作者:

    彭东辉(1971−),男,博士,教授,研究方向:园林植物与应用研究(E-mail:fjpdh@126.com

  • 中图分类号: S 682.31

Tissue Culture for Rapid Propagation of A Cymbidium goeringii Hybrid

  • 摘要:   目的  筛选春兰组培各阶段的最佳培养条件,建立优良的春兰快繁体系,为春兰大规模生产开发提供科学依据。  方法  以春兰‘黄梅’ב黄荷’F1无菌播种形成的根状茎为试验材料,通过基本培养基(1/2MS、改良1/2MS、HyponexⅡ)和植物生长调节剂(6-BA、NAA、TDZ、IBA)等因子不同组合,围绕增殖、分化、生根等阶段建立其再生体系,并对培育出的组培苗进行移栽练苗。  结果  增殖最适培养基为3.0 g·L−1 HyponexⅡ+0.5 mg·L−1 6-BA+3.0 mg·L−1 NAA+1.0 g·L−1 活性炭(AC)+30.0 g·L−1 白砂糖(Su)+7.0 g·L−1 琼脂(Ag),生长速度和增殖系数分别为7.31和6.02;分化最适培养基为2.0 mg·L−1 6-BA+0.3 mg·L−1 NAA+30.0 g·L−1 Su+7.0 g·L−1 Ag,分化率、芽诱导个数和株高分别为90.00%、5.44个和2.53 cm;生根最适培养基为1.5 mg·L−1 IBA+2.0 g·L−1蛋白胨+1.0 g·L−1 AC+25.0 g·L−1 Su+7.0 g·L−1 Ag,生根率、生根数和平均根长分别为100.00%、8.03条和3.00 cm;春兰组培苗练苗移栽60 d后存活率达96.53%。  结论  新型基本培养基HyponexⅡ(3.0 g·L−1)对春兰增殖培养有高效促进作用,高水平的IBA(1.5 mg·L−1)有利于春兰组培苗生根壮苗。
  • 图  1  离体培养各个阶段

    注:A为增殖培养第1 d;B为增殖培养第15 d;C为增殖培养120 d;D为分化培养第1 d;E为分化培养第15 d;F为分化培养120 d;G为生根培养120 d;H为组培苗练苗移栽60 d。

    Figure  1.  Tissue cultures at various development stages

    Note: A is multiplication culture on 1st day; B, 15th day; and, C, 120th day. D is differentiation culture on 1st day; E, 15th day; and, F, 120th day. G is rooting culture on 120th day. H indicates plantlets on 60th day after transplantation.

    表  1  根状茎增殖培养配方正交设计L9(34

    Table  1.   Orthogonal design L9 (34) on culture media for rhizome propagation

    水平
    Level
    因素 Factor
    基本培养基 Basic medium6-BA/(mg·L−1)NAA/(mg·L−1)
    11/2MS0.12.0
    2Revised 1/2MS0.33.0
    3Hyponex Ⅱ3.0 g·L−10.55.0
    注:改良1/2MS为不含有微量元素的1/2MS培养基。
    Note: Modified ½ MS was regular ½ MS without added microelements.
    下载: 导出CSV

    表  2  根状茎分化培养试验设计

    Table  2.   Experimental design on culture media for rhizome differentiation

    处理号 NumberNAA/(mg·L−1)6-BA/(mg·L−1)TDZ/(mg·L−1)
    10.31.00.0
    20.32.00.0
    30.33.00.0
    40.30.00.5
    50.30.01.0
    60.30.01.5
    70.51.00.0
    80.52.00.0
    90.53.00.0
    100.50.00.5
    110.50.01.0
    120.50.01.5
    下载: 导出CSV

    表  3  组培苗生根培养全因素设计

    Table  3.   All factor design on culture media for plantlet rooting

    处理号 NumberIBA/(mg·L−1)蛋白胨 Peptone/(g·L−1)
    10.51.0
    20.52.0
    30.53.0
    41.01.0
    51.02.0
    61.03.0
    71.51.0
    81.52.0
    91.53.0
    下载: 导出CSV

    表  4  不同处理对根状茎增殖培养的影响

    Table  4.   Effects of medium composition on rhizome propagation

    处理号
    Number
    A:基本培养基
    Basic medium
    B:6-BA/(mg·L−1)C:NAA/(mg·L−1)生长速度
    Speed of growth
    增殖系数
    Proliferation coefficient
    11112.53±0.35 Bd7.40±0.42 Aa
    21224.19±0.14 Bbcdd6.69±0.0 5 Aab
    31334.76±0.31 ABbc7.46±0.66 Aa
    42122.91±0.18 Bcd6.27±0.31 Aab
    52233.46±0.15 Bbcd7.20±0.30 Aa
    62314.19±0.47 Bbcd5.85±0.07 Aab
    73135.21±0.41 ABb5.41±0.54 Ab
    83215.10±0.53 ABbc5.93±0.10 Aab
    93327.31±0.27 Aa6.02±0.82 Aab
    ka13.833.553.94Ra值排序:A>B>C
    最优水平:A3B3C2
    Rb值排序:A>C>B
    最优水平:A1B2C3
    ka23.524.254.80
    ka35.875.424.48
    Ra2.351.870.87
    kb17.186.366.39
    kb26.446.616.33
    kb35.796.456.69
    Rb1.390.250.37
    注:ka表示生长速度;Ra表示生长速度的极差;kb表示增殖系数;Rb表示增殖系数的极差。大写字母表示在0.01水平下的差异,小写字母表示0.05水平下的差异,下同。
    Note: ka stands for speed of growth; Ra, variance of ka; kb, multiplication coefficient; and, Rb, variance of kb. Capital letters indicate significantly different at P<0.01, lowercase significantly different at P<0.05. Same for Table 5&6.
    下载: 导出CSV

    表  5  NAA、6-BA和TDZ对根状茎芽分化的影响

    Table  5.   Effects of varied concentrations of NAA, 6-BA, and TDZ on rhizome differentiation

    处理号 Number分化率 Differentiation rate /%芽诱导个数 Number of Induced bud /个株高 Plant height/cm
    198.00±6.32 Aa4.07±0.53 BCDEbcde2.70±0.81 Aa
    290.00±10.44 ABabcd5.44±0.78 Aa2.53±0.75 Aa
    394.55±12.93 ABab4.90±0.52 ABab1.51±0.42 DEFcd
    478.08±19.10 Bcd4.48±0.71 ABCDbc1.70±0.36 CDEc
    591.67±13.37 ABabc4.78±0.63 ABCab1.26±0.27 EFde
    677.14±15.41 Bd4.07±0.50 BCDEbcde1.06±0.16 Fe
    794.00±13.50 ABab3.55±0.36 CDEcde2.77±0.90 Aa
    887.27±13.48 ABabcd3.31±0.33 DEde2.49±0.79 ABa
    980.00±16.33 ABcd3.09±0.33 Ee2.07±0.55 BCb
    1046.00±9.66 Ce3.15±0.48 Ee1.77±0.47 CDbc
    1188.33±10.30 ABabcd4.18±0.47 BCDEbcd1.51±0.30 DEFcd
    1281.43±11.43 ABbcd3.80±0.45 BCDEcde1.47±0.33 DEFcd
    下载: 导出CSV

    表  6  不同IBA和蛋白胨对组培苗生根的影响

    Table  6.   Effects of varied concentrations of IBA and peptone on rooting of seedlings

    处理号 Number生根率 Rooting rate/%生根数 Number of roots/条平均根长 Average root length /cm
    172.73±16.61 Aa5.25±0.76 ABb2.65±1.19 Bc
    282.86±12.87 Aa5.89±1.16 ABab2.80±1.20 ABbc
    383.33±18.25 Aa4.53±0.61 Bb2.77±1.13 Bbc
    489.47±15.29 Aa5.25±1.00 ABb2.75±1.30 Bbc
    588.24±15.94 Aa5.29±1.79 ABb2.91±1.27 ABabc
    677.42±12.62 Aa6.87±1.09 ABab3.18±1.12 Aa
    797.14±9.24 Aa6.46±1.17 ABab3.19±1.20 Aa
    8100.00±0 Aa8.03±1.38 Aa3.00±1.16 ABab
    996.43±7.91 Aa6.31±1.37 ABab2.75±1.08 Bbc
    下载: 导出CSV
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出版历程
  • 收稿日期:  2019-05-15
  • 修回日期:  2019-07-18
  • 刊出日期:  2019-09-01

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