贵州省水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性

刘世江; 丁怡; 赵琪君; 李明; 文小东; 宋星陈; 李荣玉

doi:10.19303/j.issn.1008-0384.2019.11.009

贵州省水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性

doi: 10.19303/j.issn.1008-0384.2019.11.009

刘世江^{1, 2,},
丁怡^{1, 2},
赵琪君^{1, 2},
李明^{1, 2},
文小东^{1, 2},
宋星陈^{1, 2},
李荣玉^{1, 2, 3, ,}

1.
贵州大学作物保护研究所，贵州　贵阳　550025
2.
贵州大学猕猴桃工程技术研究中心，贵州　贵阳　550025
3.
贵州省山地农业病虫害重点实验室，贵州　贵阳　550025

基金项目: 国家重点研发计划项目（2016YFD0200500）；国家自然科学基金（31701816）；贵州省科学技术基金计划项目（黔科合基础[2017]1083）；贵州大学引进人才科研项目[贵大人基合字（2015）07号）]；黔科合平台人才项目（[2017]5788号）

详细信息

作者简介:
刘世江（1995−），男，硕士研究生，研究方向：农产品质量安全（E-mail：942216633@qq.com）

通讯作者:
李荣玉（1984−），男，博士，副教授，研究方向：农药毒理学与分子生物学及农产品质量安全（E-mail：lirongyu0328@126.com）

中图分类号: S 482.2
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出版历程
- 收稿日期: 2019-06-26
- 修回日期: 2019-09-27
- 刊出日期: 2019-11-01

Sensitivities of Rhizoctonia solani to Propiconazole and Pyraclostrobin on Rice Plants in Guizhou Province

LIU Shi-jiang^{1, 2
,},
DING Yi^{1, 2},
ZHAO Qi-jun^{1, 2},
LI Ming^{1, 2},
WEN Xiao-dong^{1, 2},
SONG Xing-chen^{1, 2},
LI Rong-yu^{1, 2, 3
, ,}

1.
Institute of Crop Protection, Guizhou University, Guiyang, Guizhou　550025, China
2.
Engineering and Technology Research Center of Kiwifruit, Guiyang, Guizhou　550025, China
3.
Provincial Key Laboratory for Agricultural Pest Management in Mountainous Region, Guizhou University, Guiyang, Guizhou　550025, China

摘要

摘要: 目的明确贵州省水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性，为水稻纹枯病的科学防治及合理用药提供参考。方法采用菌丝生长速率法测定从贵州省4个地区采集分离的52株水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性及药剂间的相关性。结果供试菌株对丙环唑的EC₅₀值为0.058～2.381 μg·mL⁻¹，均值为0.422 μg·mL⁻¹；供试菌株对吡唑醚菌酯的EC₅₀值范围从0.077～0.936 μg·mL⁻¹，均值为0.313 μg·mL⁻¹。抗性水平分析表明，湄潭地区的水稻纹枯病菌对丙环唑和吡唑醚菌酯出现了低抗水平的菌株，抗性频率分别为16.7%、13.3%。聚类分析表明，水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性差异与菌株地理来源无明显相关性，且这两种药剂的敏感性之间也无显著相关性。结论供试菌株中多数菌株对丙环唑和吡唑醚菌酯的敏感性较高。药剂间未产生交互抗性，生产上可以交替使用。
- 纹枯病菌 /
- 丙环唑 /
- 吡唑醚菌酯 /
- 敏感性 /
- 交互抗性
Abstract: Objective Sensitivities of Rhizoctonia solani on rice to propiconazole and pyraclostrobin were determined for proper pesticide application on the disease control in Guizhou. Method Mycelium growth rate was used as the criterion for testing the pesticide sensitivities of 52 strains of R. solani collected from 4 regions in the province. Result The EC₅₀ of propiconazole on the pathogens ranged from 0.058 μg·mL⁻¹ to 2.381 μg·mL⁻¹, averaging 0.422 μg·mL⁻¹. That of pyraclostrobin were between 0.077 μg·mL⁻¹ and 0.936 μg·mL⁻¹, averaging 0.313 μg·mL⁻¹. Strains of low resistance to the pesticides were found in Meitan area of the province with a resistance frequency of 16.7% on propiconazole and 13.3% on pyraclostrobin. A cluster analysis showed no significant correlation between the pesticide sensitivities and the geographical origins of the pathogens, nor between the two pesticides. Conclusion A large number of the R. solani isolates in the study were highly sensitive to propiconazole and pyraclostrobin, and no apparent cross-resistance observed between the two fungicides. Hence, alternative application of them for disease control was deemed appropriate.
- Rhizoctonia solani /
- propiconazole /
- pyraclostrobin /
- pesticide sensitivity /
- cross-resistance

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图 1 水稻纹枯病菌对丙环唑（A）和吡唑醚菌酯（B）的敏感性

Figure 1. Sensitivities of R. solani to propiconazole (A) and pyraclostrobin (B)

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图 2 水稻纹枯病菌对丙环唑（A）和吡唑醚菌酯（B）的敏感性频率分布

Figure 2. Frequency distributions of sensitivities of R. solani to propiconazole (A) and pyraclostrobin (B)

下载: 全尺寸图片幻灯片

图 3 水稻纹枯病菌对丙环唑（A）和吡唑醚菌酯（B）的EC₅₀值系统聚类分析

注：A代表丙环唑聚类分析，B代表吡唑醚菌酯聚类分析

Figure 3. Cluster analysis on EC₅₀ of R. solani to propiconazole （A） and pyraclostrobin （B）

Note: A=cluster analysis for propiconazole, B=cluster analysis for pyraclostrobin

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图 4 水稻纹枯病菌对丙环唑和吡唑醚菌酯敏感性的相关性

Figure 4. Correlation on pesticide sensitivities of R. solani between propiconazole and pyraclostrobin

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表 1 不同地区纹枯病菌对丙环唑和吡唑醚菌酯的敏感性

Table 1. Pesticide sensitivities of R. solani from different regions

采集地 Locality	菌株数 Isolate number	丙环唑 propiconazole		吡唑醚菌酯 pyraclostrobin
采集地 Locality	菌株数 Isolate number	EC₅₀范围 Range of EC₅₀/（μg·mL⁻¹）	EC₅₀均值 Average value of EC₅₀/（μg·mL⁻¹）	EC₅₀范围 Range of EC₅₀/（μg·mL⁻¹）	EC₅₀均值 Average value of EC₅₀/（μg·mL⁻¹）
惠水 HS Huishui	13	0.065～0.519	（0.256±0.048）b	0.077～0.234	（0.153±0.016）b
湄潭 MT Meitang	18	0.077～2.381	（0.623±0.169）a	0.156～0.936	（0.525±0.062）a
黄平 HP Huangping	12	0.066～0.209	（0.140±0.013）b	0.126～0.383	（0.241±0.020）b
三穗 SS Sansui	9	0.498～1.060	（0.635±0.056）a	0.127～0.317	（0.217±0.019）b
注：同列数字后不同字母表示经新复极差法检验差异显著（P＜0.05）。 Note: Data followed by the different letters are significantly by new multiple range test（ P ＜0.05）.

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表 2 不同地区水稻纹枯病菌对丙环唑和吡唑醚菌酯的抗性水平

Table 2. Pesticide resistances of R. solani from different regions

采集地 Locality	菌株数 Isolate number	丙环唑				吡唑醚菌酯
		不同抗性水平菌株频率 Frequencies at different resistance level/%		抗性频率 Resistance frequencies/%	抗性水平范围 Range of resistance level	不同抗性水平菌株频率 Frequencies at different resistance level/%		抗性频率 Resistance frequencies/%	抗性水平范围 Range of resistance level
		Rf＜3	3＜Rf≤10	抗性频率 Resistance frequencies/%	抗性水平范围 Range of resistance level	Rf＜3	3＜Rf≤10	抗性频率 Resistance frequencies/%	抗性水平范围 Range of resistance level
惠水 HS Huishui	13	100	0	0	0.14～1.23	100	0	0	0.24～0.75
湄潭 MT Meitang	18	83.3	16.7	16.7	0.18～5.64	86.6	13.3	13.3	0.50～3.01
黄平 HP Huangping	12	100	0	0	0.16～0.50	100	0	0	0.40～1.22
三穗 SS Sansui	9	100	0	0	1.18～2.51	100	0	0	0.41～1.01

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